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http://dx.doi.org/10.5483/BMBRep.2012.45.12.138

Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells  

Ryu, Jae-Sung (Department of Biological Science, College of Natural Sciences, Wonkwang University)
Chang, Kyu-Tae (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Ju-Taek (Department of Biological Science, College of Natural Sciences, Wonkwang University)
Lim, Malg-Um (Department of Biological Science, College of Natural Sciences, Wonkwang University)
Min, Hyun-Ki (Department of Biological Science, College of Natural Sciences, Wonkwang University)
Na, Yoon-Ju (Department of Biological Science, College of Natural Sciences, Wonkwang University)
Lee, Su-Bin (Department of Biological Science, College of Natural Sciences, Wonkwang University)
Moussavou, Gislain (Department of Biological Science, College of Natural Sciences, Wonkwang University)
Kim, Sun-Uk (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Ji-Su (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Ko, Kinarm (Center for Stem Cell Research, Institute of Biomedical Sciences and Technology, Konkuk University)
Ko, Kisung (Department of Medicine, College of Medicine, Chung-Ang University)
Hwang, Kyung-A (Department of Agrofood Resources, National Academy of Agricultural Science, RDA)
Jeong, Eun-Jeong (Center for Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Jeong-Woong (Center for Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Choo, Young-Kug (Department of Biological Science, College of Natural Sciences, Wonkwang University)
Publication Information
BMB Reports / v.45, no.12, 2012 , pp. 713-718 More about this Journal
Abstract
Gangliosides play important roles in the control of several biological processes, including proliferation and transmembrane signaling. In this study, we demonstrate the effect of ganglioside GM1 on the proliferation of mouse induced pluripotent stem cells (miPSCs). The proliferation rate of miPSCs was lower than in mouse embryonic stem cells (mESCs). Fluorescence activated cell sorting analysis showed that the percentage of cells in the G2/M phase in miPSCs was lower than that in mESCs. GM1 was expressed in mESCs, but not miPSCs. To confirm the role of GM1 in miPSC proliferation, miPSCs were treated with GM1. GM1-treated miPSCs exhibited increased cell proliferation and a larger number of cells in the G2/M phase. Furthermore, phosphorylation of mitogen-activated protein kinases was increased in GM1-treated miPSCs.
Keywords
Cell cycle; Ganglioside GM1; MAP kinase; Mouse induced pluripotent stem cells (miPSCs); Proliferation;
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Times Cited By KSCI : 4  (Citation Analysis)
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