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http://dx.doi.org/10.5483/BMBRep.2011.44.9.607

Activation of Toll-like receptor 9 and production of epitope specific antibody by liposome-encapsulated CpG-DNA  

Kim, Dong-Bum (Department of Microbiology, College of Medicine, Hallym University)
Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University)
Lee, Young-Hee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University)
Publication Information
BMB Reports / v.44, no.9, 2011 , pp. 607-612 More about this Journal
Abstract
Several investigators have shown that CpG-DNA has outstanding effects as a Th1-responsive adjuvant and that its potent adjuvant effects are enhanced by encapsulation with a liposome of proper composition. In this study, we showed that encapsulation with phosphatidyl-${\beta}$-oleoyl-${\gamma}$-palmitoyl ethanolamine (DOPE): cholesterol hemisuccinate (CHEMS) complex enhances the immunostimulatory activity of CpG DNA and the binding of CpG-DNA to TLR9. We also examined involvement of myeloid differentiation protein (MyD88) and NF-${\kappa}B$ activation in liposome-encapsulated CpG-DNA-induced IL-8 promoter activation. In this manuscript, the natural phosphodiester bond CpG-DNA encapsulated by DOPE : CHEMS complex is designated as Lipoplex(O). Importantly, we successfully screened B cell epitopes of envelope protein (E protein) of hepatitis C virus (HCV-E) and attachment glycoprotein G of human respiratory syncytial virus (HRSV-G) by immunization with complexes of several peptides and Lipoplex(O) without carriers. Therefore, Lipoplex(O) is potentially applicable as a universal adjuvant for peptide-based epitope screening and antibody production.
Keywords
Adjuvant; CpG-DNA; Epitope; Lipoplex(O);
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