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http://dx.doi.org/10.5483/BMBRep.2011.44.8.541

FSCB phosphorylation in mouse spermatozoa capacitation  

Liu, Shun-Li (Department of Urology, Daping Hospital, The Third Military Medical University)
Ni, Bing (Institute of Immunology PLA, Third Military Medical University)
Wang, Xiang-Wei (Department of Urology, Xinqiao Hospital, The Third Military Medical University)
Huo, Wen-Qian (Department of Urology, Daping Hospital, The Third Military Medical University)
Zhang, Jun (Department of Urology, Daping Hospital, The Third Military Medical University)
Tian, Zhi-Qiang (Institute of Immunology PLA, Third Military Medical University)
Huang, Ze-Min (Institute of Immunology PLA, Third Military Medical University)
Tian, Yi (Institute of Immunology PLA, Third Military Medical University)
Tang, Jun (Institute of Immunology PLA, Third Military Medical University)
Zheng, Yan-Hua (Institute of Immunology PLA, Third Military Medical University)
Jin, Feng-Shuo (Department of Urology, Daping Hospital, The Third Military Medical University)
Li, Yan-Feng (Department of Urology, Daping Hospital, The Third Military Medical University)
Publication Information
BMB Reports / v.44, no.8, 2011 , pp. 541-546 More about this Journal
Abstract
It is generally accepted that spermatozoa capacitation is associated with protein kinase A-mediated tyrosine phosphorylation. In our previous study, we identified the fibrous sheath CABYR binding protein (FSCB), which was phosphorylated by PKA. However, the phosphorylation status of FSCB protein during spermatozoa capacitation should be further investigated. To this aim, in this study, we found that phosphorylation of this 270-kDa protein occurred as early as 1 min after mouse spermatozoa capacitation, which increased over time and remained stable after 60 min. Immunoprecipitation assays demonstrated that the tyrosine and Ser/Thr phosphorylation of FSCB occurred during spermatozoa capacitation. The extent of phosphorylation and was closely associated with the PKA activity and spermatozoa motility characteristics. FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine-phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation.
Keywords
Capacitation; Fibrous sheath; Phosphorylation; Protein kinase A; Spermatozoa;
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