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http://dx.doi.org/10.5483/BMBRep.2011.44.7.484

Transduced Tat-Annexin protein suppresses inflammation-associated gene expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells  

Lee, Sun-Hwa (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University)
Kim, Dae-Won (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University)
Back, Su-Sun (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University)
Hwang, Hyun-Sook (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University)
Park, Eun-Young (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University)
Kang, Tae-Cheon (Department of Anatomy and Neurobiology, College of Medicine, Hallym University)
Kwon, Oh-Shin (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
Park, Jong-Hoon (Department of Biological Sciences, Sookmyung Women's University)
Cho, Sung-Woo (Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine)
Han, Kyu-Hyung (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University)
Park, Jin-Seu (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University)
Eum, Won-Sik (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University)
Choi, Soo-Young (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University)
Publication Information
BMB Reports / v.44, no.7, 2011 , pp. 484-489 More about this Journal
Abstract
Annexin-1 (ANX1) is an anti-inflammatory protein as well as an important modulator in inflammation. However, the precise action of ANX1 remains unclear. To elucidate the protective effects of ANX1 on lipopolysaccharide (LPS)-induced murine macrophage Raw 264.7 cells, we constructed a cell-permeable Tat-ANX1 protein. The transduced Tat-ANX1 protein markedly inhibited the expression of cyclooxygenase-2, production of prostaglandin $E_2$, and generation of pro-inflammatory cytokines in the cells. Furthermore, transduced Tat-ANX1 protein caused a significant reduction in the activation of nuclear factor-kappa B (NF-${\kappa}B$) and mitogen-activated protein kinase (MAPK). The results indicate that Tat-ANX1 inhibits the production of inflammatory response cytokines and enzymes by blocking NF-${\kappa}B$ and MAPK. Therefore, Tat-ANX1 protein may be useful as a therapeutic agent against various inflammatory diseases.
Keywords
Cytokine; Inflammation; MAPK; Protein therapy; Tat- ANX1;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 12  (Related Records In Web of Science)
Times Cited By SCOPUS : 12
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