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http://dx.doi.org/10.5483/BMBRep.2011.44.7.462

Suppression of TNF-alpha-induced MMP-9 expression by a cell-permeable superoxide dismutase in keratinocytes  

Song, Ha-Yong (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University)
Ju, Sung-Mi (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University)
Goh, Ah-Ra (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University)
Kwon, Dong-Joo (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University)
Choi, Soo-Young (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University)
Park, Jin-Seu (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University)
Publication Information
BMB Reports / v.44, no.7, 2011 , pp. 462-467 More about this Journal
Abstract
Up-regulation of selected matrix metalloproteinases (MMPs) such as MMP-9 contributes to inflammatory processes during the development of various skin diseases, such as atopic dermatitis. In this study, we examined the effect of a cell-permeable superoxide dismutase (Tat-SOD) on TNF-${\alpha}$-induced MMP-9 expression in human keratinocyte cells (HaCaT). When Tat-SOD was added to the culture medium of HaCaT cells, it rapidly entered the cells in dose- and time-dependent manners. Tat-SOD decreased TNF-${\alpha}$-induced reactive oxygen species (ROS) generation. Tat-SOD also inhibited TNF-${\alpha}$-induced NF-${\kappa}B$ DNA binding activity. Treatment of HaCaT cells with Tat-SOD significantly inhibited TNF-${\alpha}$-induced mRNA and protein expression of MMP-9, as measured by RT-PCR and Western blot analysis. In addition, Tat-SOD suppressed TNF-${\alpha}$-induced gelatinolytic activity of MMP-9. Taken together, our results indicate that Tat-SOD can suppress TNF-${\alpha}$-induced MMP-9 expression via ROS-NF-${\kappa}B$-dependent mechanisms in keratinocytes, and therefore can be used as an immunomodulatory agent against inflammatory skin diseases related to oxidative stress.
Keywords
Inflammation; Keratinocyte; MMP-9; NF-${\kappa}B$; SOD; TNF-${\alpha}$;
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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1 Okayama, Y. (2005) Oxidative stress in allergic and inflammatory skin diseases. Curr. Drug Targets Inflamm. Allergy 4, 517-519.   DOI
2 Greenwald, R. A. (1990) Superoxide dismutase and catalase as therapeutic agents for human diseases. A critical review. Free Radic. Biol. Med. 8, 201-209.   DOI   ScienceOn
3 Bickers, D. R. and Athar, M. (2006) Oxidative stress in the pathogenesis of skin disease. J. Invest. Dermatol. 126, 2565-2575.   DOI   ScienceOn
4 Vincenti, M. P. and Brinckerhoff, C. E. (2007) Signal transduction and cell-type specific regulation of matrix metalloproteinase gene expression: can MMPs be good for you? J. Cell. Physiol. 213, 355-364.   DOI   ScienceOn
5 Park, L. J., Ju, S. M., Song, H. Y., Lee, J. A., Yang, M. Y., Kang, Y. H., Kwon, H. J., Kim, T. Y., Choi, S. Y. and Park, J. (2006) The enhanced monocyte adhesiveness after UVB exposure requires ROS and NF-kappaB signaling in human keratinocyte. J. Biochem. Mol. Biol. 39, 618-625.   DOI   ScienceOn
6 Seo, W. Y., Goh, A. R., Ju, S. M., Song, H. Y., Kwon, D. J., Jun, J. G., Kim, B. C., Choi, S. Y. and Park, J. (2011) Celastrol induces expression of heme oxygenase-1 through ROS/Nrf2/ARE signaling in the HaCaT cells. Biochem. Biophys. Res. Commun. 407, 535-540.   DOI   ScienceOn
7 Ju, S. M., Song, H. Y., Lee, J. A., Lee, S. J., Choi, S. Y. and Park, J. (2009) Extracellular HIV-1 Tat up-regulates expression of matrix metalloproteinase-9 via a MAPK-NFkappaB dependent pathway in human astrocytes. Exp. Mol. Med. 41, 86-93.   DOI   ScienceOn
8 Young, C. N., Koepke, J. I., Terlecky, L. J., Borkin, M. S., Boyd Savoy, L. and Terlecky, S. R. (2008) Reactive oxygen species in tumor necrosis factor-alpha-activated primary human keratinocytes: implications for psoriasis and inflammatory skin disease. J. Invest. Dermatol. 128, 2606-2614.   DOI   ScienceOn
9 Brenneisen, P., Sies, H. and Scharffetter-Kochanek, K. (2001) Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events. Ann. N. Y. Acad. Sci. 973, 31-43.
10 Schulze-Osthoff, K., Bauer, M. K., Vogt, M. and Wesselborg, S. (1997) Oxidative stress and signal transduction. Int. J. Vitam. Nutr. Res. 67, 336-342.
11 Schwarze, S. R. and Dowdy, S. F. (2000) In vivo protein transduction: intracellular delivery of biologically active proteins, compounds and DNA. Trends Pharmacol. Sci. 21, 45-48.   DOI   ScienceOn
12 Schwarze, S. R., Ho, A., Vocero-Akbani, A. and Dowdy, S. F. (1999) In vivo protein transduction: delivery of a biologically active protein into the mouse. Science 285, 1569-1572.   DOI   ScienceOn
13 Lee, J. A., Song, H. Y., Ju, S. M., Lee, S. J., Kwon, H. J., Eum, W. S., Jang, S. H., Choi, S. Y. and Park, J. (2009) Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells. Exp. Mol. Med. 41, 629-637.   DOI   ScienceOn
14 Lee, J. A., Song, H. Y., Ju, S. M., Lee, S. J., Seo, W. Y., Sin, D. H., Goh, A. R., Choi, S. Y. and Park, J. (2010) Suppression of inducible nitric oxide synthase and cyclooxygenase- 2 by cell-permeable superoxide dismutase in lipopolysaccharide-stimulated BV-2 microglial cells. Mol. Cells 29, 245-250.   DOI   ScienceOn
15 Devillers, A. C., van Toorenenbergen, A. W., Klein, Heerenbrink, G. J., Muldert, P. G. and Oranje, A. P. (2007) Elevated levels of plasma matrix metalloproteinase- 9 in patients with atopic dermatitis: a pilot study. Clin. Exp. Dermatol. 32, 311-313.   DOI   ScienceOn
16 Song, H. Y., Lee, J. A., Ju, S. M., Yoo, K. Y., Won, M. H., Kwon, H. J., Eum, W. S., Jang, S. H., Choi, S. Y. and Park, J. (2008) Topical transduction of superoxide dismutase mediated by HIV-1 Tat protein transduction domain ameliorates 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice. Biochem. Pharmacol. 75, 1348-1357.   DOI   ScienceOn
17 Pilcher, B. K., Wang, M., Qin, X. J., Parks, W. C., Senior, R. M. and Welgus, H. G. (1999) Role of matrix metalloproteinases and their inhibition in cutaneous wound healing and allergic contact hypersensitivity. Ann. N. Y. Acad. Sci. 878, 12-24.   DOI
18 Hofmann, U. B., Westphal, J. R., Van Muijen, G. N. and Ruiter, D. J. (2000) Matrix metalloproteinases in human melanoma. J. Invest. Dermatol. 115, 337-344.   DOI   ScienceOn
19 Harper, J. I., Godwin, H., Green, A., Wilkes, L. E., Holden, N. J., Moffatt, M., Cookson, W. O., Layton, G. and Chandler, S. (2010) A study of matrix metalloproteinase expression and activity in atopic dermatitis using a novel skin wash sampling assay for functional biomarker analysis. Br. J. Dermatol. 162, 397-403.   DOI   ScienceOn
20 Bahar-Shany, K., Ravid, A. and Koren, R. (2010) Upregulation of MMP-9 production by TNFalpha in keratinocytes and its attenuation by vitamin D. J. Cell. Physiol. 222, 729-737.
21 Kim, H. H., Lee, Y., Eun, H. C. and Chung, J. H. (2008) Eicosapentaenoic acid inhibits TNF-alpha-induced matrix metalloproteinase-9 expression in human keratinocytes, HaCaT cells. Biochem. Biophys. Res. Commun. 368, 343-349.   DOI   ScienceOn
22 Lawley, T. J. and Kubota, Y. (1991) Cell adhesion molecules and cutaneous inflammation. Semin. Dermatol. 10, 256-259.
23 Dhar, A., Young, M. R. and Colburn, N. H. (2002) The role of AP-1, NF-kappaB and ROS/NOS in skin carcinogenesis: the JB6 model is predictive. Mol. Cell. Biochem. 234-235, 185-193.   DOI   ScienceOn
24 Kohler, H. B., Huchzermeyer, B., Martin, M., De Bruin, A., Meier, B. and Nolte, I. (2001) TNF-alpha dependent NF-kappa B activation in cultured canine keratinocytes is partly mediated by reactive oxygen species. Vet. Dermatol. 12, 129-137.   DOI   ScienceOn
25 Deane, J. A. and Hickey, M. J. (2009) Molecular mechanisms of leukocyte trafficking in T-cell-mediated skin inflammation: insights from intravital imaging. Expert. Rev. Mol. Med. 11, e25.   DOI   ScienceOn