[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5483/BMBRep.2011.44.6.399

Anti-inflammatory effects of ethanol extract from Orostachys japonicus on modulation of signal pathways in LPS-stimulated RAW 264.7 cells

Jeong, Jae-Han (Department of Biomedical Laboratory Science, Inje University)
Ryu, Deok-Seon (Department of Smart Foods and Drugs, Inje University)
Suk, Dong-Hee (Department of Biomedical Laboratory Science, Inje University)
Lee, Dong-Seok (Department of Smart Foods and Drugs, Inje University)

Publication Information

BMB Reports / v.44, no.6, 2011 , pp. 399-404 More about this Journal

Abstract

In this study, powder of Orostachys japonicus A. Berger (O. japonicus) was extracted with 95% ethyl alcohol and fractionated using a series of organic solvents, including n-hexane (hexane), dichloromethane (DCM), ethylacetate (EtOAc), n-butanol (BuOH), and water ( $H_2O$ ). We investigated the anti-inflammatory effects of these O. japonicus extracts on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Their effects on the expression of inflammatory mediators and transcription factors were analyzed by Western blotting. DCM fraction significantly inhibited formation of reactive oxygen species (ROS) as well as nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. Phosphorylation of the pro-inflammatory transcription factor complex nuclear factor-kappa B (NF- ${\kappa}$ B) p65 and expression of inducible nitric oxide synthase (iNOS), one of its downstream proteins, were also suppressed by DCM fraction. These effects were regulated by upsteam proteins in the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase/Akt (PI3K/Akt) signaling pathways. Taken together, our data suggest that O. japonicus could be used as a potential source for anti-inflammatory agents.

Keywords

Anti-inflammation; Nuclear factor-kappa B (NF- ${\kappa}$ B); Orostachys japonicus; RAW 264.7 cell; Signaling pathway;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)
Times Cited By Web Of Science : 5 (Related Records In Web of Science)
Times Cited By SCOPUS : 7

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3	Anti-oxidizing effect of the dichloromethane and hexane fractions from Orostachys japonicus in LPS-stimulated RAW 264.7 cells via upregulation of Nrf2 expression and activation of MAPK signaling pathway / [Lee, Hyeong-Seon;Lee, Gyeong-Seon;Kim, Seon-Hee;Kim, Hyun-Kyung;Suk, Dong-Hee;Lee, Dong-Seok;] / BMB Reports
4	Oleanolic acid regulates NF-κB signaling by suppressing MafK expression in RAW 264.7 cells / [Hwang, Yu-Jin;Song, Jaewhan;Kim, Haeng-Ran;Hwang, Kyung-A;] / BMB Reports
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