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http://dx.doi.org/10.5483/BMBRep.2011.44.6.381

Human HS1BP3 induces cell apoptosis and activates AP-1  

Shi, Taiping (Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center)
Xie, Jieshi (Chinese National Human Genome Center)
Xiong, Ying (Chinese National Human Genome Center)
Deng, Weiwei (Chinese National Human Genome Center)
Guo, Jinhai (Chinese National Human Genome Center)
Wang, Feng (Chinese National Human Genome Center)
Ma, Dalong (Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center)
Publication Information
BMB Reports / v.44, no.6, 2011 , pp. 381-386 More about this Journal
Abstract
In the present study, we characterized the function of HS1-binding protein 3 (HS1BP3), which is mutated in essential tremor and may be involved in lymphocyte activation. We found that HS1BP3 localized to the mitochondria and endoplasmic reticulum partially. Overexpression of HS1BP3 induced apoptosis in HEK293T and HeLa cell lines. When these cell lines were transfected with HS1BP3, they exhibited nuclear DNA condensation, externalization of phosphatidylserine (PS), and cleavage of poly ADP ribose polymerase (PARP). Furthermore, suppression of HS1BP3 or HS1 expression attenuates HS1BP3 induced apoptosis. In addition, HS1BP3 enhanced activator protein 1 (AP-1)-mediated transcription in a dose-dependent manner. Therefore, we conclude that HS1BP3 regulates apoptosis via HS1 and stimulates AP-1-mediated transcription.
Keywords
AP-1; Apoptosis; HS1; HS1BP3;
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1 Chen, Y. R., Kori, R., John, B. and Tan, T. H. (2001) Caspase-mediated cleavage of actin-binding and SH3-domaincontaining proteins cortactin, HS1, and HIP-55 during apoptosis. Biochem. Biophys. Res. Commun. 288, 981-989.   DOI   ScienceOn
2 Ensenat, D., Yao, Z., Wang, X. S., Kori, R., Zhou, G., Lee, S. C. and Tan, T. H. (1999) A novel Src homology 3 domain- containing adaptor protein, HIP-55, that interacts with hematopoietic progenitor kinase 1. J. Biol. Chem. 274, 33945-33950.   DOI
3 Tournier, C., Hess, P., Yang, D. D., Xu, J., Turner, T. K., Nimnual, A., Bar-Sagi, D., Jones, S. N., Flavell, R. A. and Davis, R. J. (2000) Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway. Science 288, 870-874.   DOI   ScienceOn
4 Lei, K., Nimnual, A., Zong, W. X., Kennedy, N. J., Flavell, R. A., Thompson, C. B., Bar-Sagi, D. and Davis, R. J. (2002) The Bax subfamily of Bcl2-related proteins is essential for apoptotic signal transduction by c-Jun NH(2)-terminal kinase. Mol. Cell. Biol. 22, 4929-4942.   DOI
5 Ham, J., Babij, C., Whitfield, J., Pfarr, C. M., Lallemand, D., Yaniv, M. and Rubin, L. L. (1995) A c-Jun dominant negative mutant protects sympathetic neurons against programmed cell death. Neuron 14, 927-939.   DOI   ScienceOn
6 Estus, S., Zaks, W. J., Freeman, R. S., Gruda, M., Bravo, R. and Johnson, E. M. Jr. (1994) Altered gene expression in neurons during programmed cell death: identification of c-jun as necessary for neuronal apoptosis. J. Cell Biol. 127, 1717-1727.   DOI
7 Bossy-Wetzel, E., Bakiri, L. and Yaniv, M. (1997) Induction of apoptosis by the transcription factor c-Jun. EMBO J. 16, 1695-1709.   DOI   ScienceOn
8 Sawai, H., Okazaki, T., Yamamoto, H., Okano, H., Takeda, Y., Tashima, M., Sawada, H., Okuma, M., Ishikura, H., Umehara, H. and Domae, N. (1995) Requirement of AP-1 for ceramide-induced apoptosis in human leukemia HL-60 cells. J. Biol. Chem. 270, 27326-27331.   DOI   ScienceOn
9 Higgins, J. J., Lombardi, R. Q., Pucilowska, J., Jankovic, J., Tan, E. K. and Rooney, J. P. (2005) A variant in the HS1-BP3 gene is associated with familial essential tremor. Neurology 64, 417-421.   DOI   ScienceOn
10 Fukuda, T., Kitamura, D., Taniuchi, I., Maekawa, Y., Benhamou, L. E., Sarthou, P. and Watanabe, T. (1995) Restoration of surface IgM-mediated apoptosis in an anti-IgM-resistant variant of WEHI-231 lymphoma cells by HS1, a protein-tyrosine kinase substrate. Proc. Natl. Acad. Sci. U.S.A. 92, 7302-7306.   DOI
11 Yamanashi, Y., Fukuda, T., Nishizumi, H., Inazu, T., Higashi, K., Kitamura, D., Ishida, T., Yamamura, H., Watanabe, T. and Yamamoto, T. (1997) Role of tyrosine phosphorylation of HS1 in B cell antigen receptor-mediated apoptosis. J. Exp. Med. 185, 1387-1392.   DOI   ScienceOn
12 Muzio, M., Scielzo, C., Frenquelli, M., Bachi, A., De Palma, M., Alessio, M., Ghia, P. and Caligaris-Cappio, F. (2007) HS1 complexes with cytoskeleton adapters in normal and malignant chronic lymphocytic leukemia B cells. Leukemia 21, 2067-2070.   DOI   ScienceOn
13 Takemoto, Y., Furuta, M., Sato, M., Kubo, M. and Hashimoto, Y. (1999) Isolation and characterization of a novel HS1 SH3 domain binding protein, HS1BP3. Int. Immunol. 11, 1957-1964.   DOI   ScienceOn
14 Keeling, B. H., Vilariño-Güell, C., Ross, O. A., Wszolek, Z. K., Uitti, R. J. and Farrer, M. J. (2009) DRD3 Ser9Gly and HS1BP3 Ala265Gly are not associated with Parkinson disease. Neurosci. Lett. 461, 74-75.   DOI   ScienceOn
15 Deng, H., Le, W. D., Guo, Y., Huang, M. S., Xie, W. J. and Jankovic, J. (2005) Extended study of A265G variant of HS1BP3 in essential tremor and Parkinson disease. Neurology 65, 651-652.   DOI   ScienceOn
16 Watabe, M., Ito, K., Masuda, Y., Nakajo, S. and Nakaya, K. (1998) Activation of AP-1 is required for bufalin-induced apoptosis in human leukemia U937 cells. Oncogene 16, 779-787.   DOI
17 Ashida, R., Tominaga, K., Sasaki, E., Watanabe, T., Fujiwara, Y., Oshitani, N., Higuchi, K., Mitsuyama, S., Iwao, H. and Arakawa, T. (2005) AP-1 and colorectal cancer. Inflammopharmacology 13, 113-125.   DOI   ScienceOn
18 Fischer, U., Michel, A. and Meese, E. U. (2005) Expression of the gene for hematopoietic cell specific protein is not restricted to cells of hematopoietic origin. Int. J. Mol. Med. 15, 611-615.
19 Herdegen, T. and Waetzig, V. (2001) AP-1 proteins in the adult brain: facts and fiction about effectors of neuroprotection and neurodegeneration. Oncogene 20, 2424-2437.   DOI
20 Angel, P., Szabowski, A. and Schorpp-Kistner, M. (2001) Function and regulation of AP-1 subunits in skin physiology and pathology. Oncogene 20, 2413-2423.   DOI
21 Matthews, C. P., Colburn, N. H. and Young, M. R. (2007) AP-1 a target for cancer prevention. Curr. Cancer Drug Targets 7, 317-324.   DOI   ScienceOn