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http://dx.doi.org/10.5483/BMBRep.2011.44.5.306

Alteration in NCX-3 immunoreactivity within the gerbil hippocampus following spontaneous seizures  

Park, Dae-Kyoon (Department of Anatomy, College of Medicine, Soonchunhyang University)
Park, Kyung-Ho (Department of Anatomy, College of Medicine, Soonchunhyang University)
Ko, Jeong-Sik (Department of Anatomy, College of Medicine, Soonchunhyang University)
Kim, Duk-Soo (Department of Anatomy, College of Medicine, Soonchunhyang University)
Publication Information
BMB Reports / v.44, no.5, 2011 , pp. 306-311 More about this Journal
Abstract
Although NCX-3 is highly expressed in the brain, the distribution of NCX-3 in the epileptic hippocampus is still controversial. Therefore, to assess the distribution and pattern of NCX-3 expression in epileptic hippocampus, we performed a comparative analysis of NCX-3 immunoreactivities in the hippocampus of seizure-resistant (SR) and seizure-sensitive (SS) gerbils. In SR gerbils, NCX-3 immunoreactivity was higher than pre-seizure SS gerbils, particularly in the pavalbumin (PV)-positive interneurons. Three h post-ictal, NCX-3 immunoreactivity in the SS gerbil hippocampus was markedly elevated to the level of SR gerbils. Six h post-ictal, the expression of NCX-3 was reduced to the level of pre-seizure SS gerbils. Therefore, the results of the present study suggest that down-regulation of NCX-3 expression in the SS gerbil hippo-campus may be involved in the hyperexcitability of SS gerbils due to an imbalance of intracellular $Na^+/Ca^{2+}$ homeostasis and $Ca^{2+}$ concentration.
Keywords
Epilepsy; Gerbil; Hippocampus; Immunohistochemistry; $Na^+/Ca^{2+}$ exchanger (NCX);
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