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http://dx.doi.org/10.5483/BMBRep.2011.44.3.199

EphrinB1 interacts with the transcriptional co-repressor Groucho/xTLE4  

Kamata, Teddy (Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick)
Bong, Yong-Sik (Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick)
Mood, Kathleen (Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick)
Park, Mae-Ja (Department of Anatomy, School of Medicine, Kyungpook National University)
Nishanian, Tagvor G. (Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick)
Lee, Hyun-Shik (Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick)
Publication Information
BMB Reports / v.44, no.3, 2011 , pp. 199-204 More about this Journal
Abstract
Ephrin signaling is involved in various morphogenetic events, such as axon guidance, hindbrain segmentation, and angiogenesis. We conducted a yeast two-hybrid screen using the intracellular domain (ICD) of EphrinB1 to gain biochemical insight into the function of the EphrinB1 ICD. We identified the transcriptional co-repressor xTLE1/Groucho as an EphrinB1 interacting protein. Whole-mount in situ hybridization of Xenopus embryos confirmed the co-localization of EphrinB1 and a Xenopus counterpart to TLE1, xTLE4, during various stages of development. The EphrinB1/xTLE4 interaction was confirmed by co-immunoprecipitation experiments. Further characterization of the interaction revealed that the carboxy-terminal PDZ binding motif of EphrinB1 and the SP domain of xTLE4 are required for binding. Additionally, phosphorylation of EphrinB1 by a constitutively activated fibroblast growth factor receptor resulted in loss of the interaction, suggesting that the interaction is modulated by tyrosine phosphorylation of the EphrinB1 ICD.
Keywords
Eph; EphrinB1; Groucho; Xenopus; xTLE4;
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