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http://dx.doi.org/10.5483/BMBRep.2011.44.2.96

Calcineurin may regulate multiple endocytic processes in C. elegans  

Song, Hyun-Ok (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine)
Ahnn, Joo-Hong (Department of Life Science, BK21 (Life Science for Global Warming Team), College of Natural Sciences, Hanyang University)
Publication Information
BMB Reports / v.44, no.2, 2011 , pp. 96-101 More about this Journal
Abstract
Calcineurin is a serine/threonine protein phosphatase controlled by $Ca^{2+}$ and calmodulin that has been implicated in various signaling pathways. Previously, we reported that calcineurin regulates coelomocyte endocytosis in Caenorhabditis elegans. So far, simple and powerful in vivo approaches have been developed to study various endocytic processes in C. elegans. Using these in vivo assays, we further analyzed the endocytic phenotypes of calcineurin mutants. We observed that the calcineurin mutants were defective in apical endocytosis in the intestine as well as synaptic vesicle recycling in the nerve cord. However, we found that calcineurin mutants displayed normal receptor-mediated endocytosis in oocytes. Therefore, our results suggest that calcineurin may regulate specific sets of endocytic processes in nematode.
Keywords
Calcineurin; C. elegans; Endocytosis; Intestine; Synaptic vesicle recycling;
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1 Schneider, W. J. (1996) Vitellogenin receptors: oocytesspecific members of the low-density lipoprotein receptor supergene family. Int. Rev. Cytol. 166, 103-137.   DOI
2 Grant, B. and Hirsh, D. (1999) Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte. Mol. Biol. Cell 10, 4311-4326.   DOI   ScienceOn
3 Grant, B., Zhang, Y., Paupard, M. C., Lin, S. X., Hall, D. H. and Hirsh, D. (2001) Evidence that RME-1, a conserved C. elegans EH-domain protein, functions in endocytic recycling. Nat. Cell Biol. 3, 573-579.   DOI   ScienceOn
4 Zhang, Y., Grant, B. and Hirsh, D. (2001) RME-8, a conserved J-domain protein, is required for endocytosis in Caenorhabditis elegans. Mol. Biol. Cell 12, 2011-2021.   DOI   ScienceOn
5 Stewart, A. A., Ingebritsen, T. S., Manalan, A., Klee, C. B. and Cohen, P. (1982) Discovery of a $Ca^{2+}$- and calmodulin-dependent protein phosphatase: probable identity with calcineurin (CaM-BP80). FEBS Lett. 137, 80-84.   DOI   ScienceOn
6 Klee, C. B., Ren, H. and Wang, X. (1998) Regulation of the calmodulin-stimulated protein phosphatase, calcineurin. J. Biol. Chem. 273, 13367-13370..0   DOI   ScienceOn
7 Crabtree, G. R. (1999) Generic signals and specific outcomes: signaling through $Ca^{2+}$, calcineurin, and NF-AT. Cell 96, 611-614.   DOI   ScienceOn
8 Klee, C. B., Draetta, G. F. and Hubbard, M. J. (1988) Calcineurin. Adv. Enzymol. Relat. Areas. Mol. Biol. 61, 149-200.
9 Stark, M. J. (1996) Yeast protein serine/threonine phosphatases: multiple roles and diverse regulation. Yeast 12, 1647-1675.   DOI   ScienceOn
10 Schreiber, S. L. and Crabtree, G. R. (1992) The mechanism of action of cyclosporin A and FK506. Immunol. Today 13, 136-142.   DOI   ScienceOn
11 Shibasaki, F. and McKeon, F. (1995) Calcineurin functions in $Ca^{2+}$-activated cell death in mammalian cells. J. Cell Biol. 131, 735-743.   DOI   ScienceOn
12 Mulkey, R. M., Endo, S., Shenolikar, S. and Malenka, R. C. (1994) Involvement of a calcineurin/inhibitor-1 phosphatase cascade in hippocampal long-term depression. Nature 369, 486-488.   DOI   ScienceOn
13 Molkentin, J. D., Lu, J. R., Antos, C. L., Markham, B., Richardson, J., Robbins, J., Grant, S. R. and Olson, E. N. (1998) A calcineurin-dependent transcriptional pathway for cardiac hypertrophy. Cell 93, 215-228.   DOI   ScienceOn
14 Klee, C. B., Crouch, T. H. and Krinks, M. H. (1979) Calcineurin: a calcium- and calmodulin-binding protein of the nervous system. Proc. Natl. Acad. Sci. U.S.A. 76, 6270-6273.   DOI   ScienceOn
15 Morck, C. and Pilon, M. (2006) C. elegans feeding defective mutants have shorter body lengths and increased autophagy. BMC Dev. Biol. 6, 39.   DOI
16 McIntire, S. L., Reimer, R. J., Schuske, K., Edwards, R. H. and Jorgensen, E. M. (1997) Identification and characterization of the vesicular GABA transporter. Nature 389, 870-876.   DOI   ScienceOn
17 White, J. G., Albertson, D. G. and Anness, M. A. (1978) Connectivity changes in a class of motoneurone during the development of a nematode. Nature 271, 764-766.   DOI   ScienceOn
18 Colavita, A., Krishna, S., Zheng, H., Padgett, R. W. and Culotti, J. G. (1998) Pioneer axon guidance by UNC-129, a C. elegans TGF-beta. Science 281, 706-709.   DOI   ScienceOn
19 Donohoe, D. R., Jarvis, R. A., Weeks, K., Aamodt, E. J. and Dwyer, D. S. (2009) Behavioral adaptation in C. elegans produced by antipsychotic drugs requires serotonin and is associated with calcium signaling and calcineurin inhibition. Neurosci. Res. 64, 280-289.   DOI   ScienceOn
20 Brenner, S. (1974) The genetics of Caenorhabditis elegans. Genetics 77, 71-94.
21 Parker, S., Walker, D. S., Ly, S. and Baylis, H. A. (2009) Caveolin-2 is required for apical lipid trafficking and suppresses basolateral recycling defects in the intestine of Caenorhabditis elegans. Mol. Biol. Cell 20, 1763-1771.   DOI   ScienceOn
22 Grant, B. D. and Sato, M. (2006) Intracellular trafficking. WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.77.1, http://www.wormbook.org.   DOI
23 Dal Santo, P., Logan, M. A., Chisholm, A. D. and Jorgensen, E. M. (1999) The inositol trisphosphate receptor regulates a 50-second behavioral rhythm in C. elegans. Cell 98, 757-767.   DOI   ScienceOn
24 McGhee, J. D. (2007) The C. elegans intestine. WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.133.1, http://www.wormbook.org.   DOI
25 Kimble, J. and Sharrock, W. J. (1983) Tissue-specific synthesis of yolk proteins in Caenorhabditis elegans. Dev. Biol. 96, 189-196.   DOI   ScienceOn
26 Fares, H. and Grant, B. (2002) Deciphering endocytosis in Caenorhabditis elegans. Traffic 3, 11-9.   DOI   ScienceOn
27 Lai, M. M., Hong, J. J., Ruggiero, A. M., Burnett, P. E., Slepnev, V. I., De Camilli, P. and Snyder, S. H. (1999) The calcineurin-dynamin 1 complex as a calcium sensor for synaptic vesicle endocytosis. J. Biol. Chem. 274, 25963-25966.   DOI
28 Nonet, M. L. (1999) Visualization of synaptic specializations in live C. elegans with synaptic vesicle protein-GFP fusions. J. Neurosci. Methods 89, 33-40.   DOI   ScienceOn
29 Tan, T. C., Valova, V. A., Malladi, C. S., Graham, M. E., Berven, L. A., Jupp, O. J., Hansra, G., McClure, S. J., Sarcevic, B., Boadle, R. A., Larsen, M. R., Cousin, M. A. and Robinson, P. J. (2003) Cdk5 is essential for synaptic vesicle endocytosis. Nat. Cell Biol. 5, 701-710.   DOI   ScienceOn
30 Jorgensen, E. M., Hartwieg, E., Schuske, K., Nonet, M. L., Jin, Y. and Horvitz, H. R. (1995) Defective recycling of synaptic vesicles in synaptotagmin mutants of Caenorhabditis elegans. Nature 378, 196-199.   DOI   ScienceOn
31 Bandyopadhyay, J., Lee, J., Lee, J. I., Yu, J. R., Jee, C., Cho, J. H., Jung, S., Lee, M. H., Zannoni, S., Singson, A., Kim, D. H., Koo, H. S. and Ahnn, J. (2002) Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is involved in movement, fertility, egg laying, and growth in Caenorhabditis elegans. Mol. Biol. Cell 13, 3281-3293.   DOI   ScienceOn
32 Kuhara, A., Inada, H., Katsura, I. and Mori, I. (2002) Negative regulation and gain control of sensory neurons by the C. elegans calcineurin TAX-6. Neuron 33, 751-763.   DOI   ScienceOn
33 Lee, J., Jee, C., Song, H. O., Bandyopadhyay, J., Lee, J. I., Yu, J. R., Park, B. J. and Ahnn, J. (2004) Opposing functions of calcineurin and CaMKII regulate G-protein signaling in egg-laying behavior of C. elegans. J. Mol. Biol. 344, 585-595.   DOI   ScienceOn
34 Song, H. O., Lee, J., Ji, Y. J., Dwivedi, M., Cho, J. H., Park, B. J. and Ahnn, J. (2010) Calcineurin regulates coelomocyte endocytosis via DYN-1 and CUP-4 in Caenorhabditis elegans. Mol. Cells 30, 255-262.   DOI   ScienceOn
35 Fares, H. and Greenwald, I. (2001) Genetic analysis of endocytosis in Caenorhabditis elegans: coelomocyte uptake defective mutants. Genetics 159, 133-145.