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http://dx.doi.org/10.5483/BMBRep.2011.44.1.58

A novel human KRAB-related zinc finger gene ZNF425 inhibits mitogen-activated protein kinase signaling pathway  

Wang, Yuequn (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Ye, Xiangli (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Zhou, Junmei (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Wan, Yongqi (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Xie, Huaping (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Deng, Yun (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Yan, Yan (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Li, Yongqing (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Fan, Xiongwei (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Yuan, Wuzhou (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Mo, Xiaoyang (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Wu, Xiushan (The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University)
Publication Information
BMB Reports / v.44, no.1, 2011 , pp. 58-63 More about this Journal
Abstract
Zinc finger (ZNF) proteins play a critical role in cell growth, proliferation, apoptosis, and intracellular signal transduction. In this paper, we cloned and characterized a novel human KRAB-related zinc finger gene, ZNF425, which encodes a protein of 752 amino acids. ZNF425 is strongly expressed in the three month old human embryos and then is almost undetectable in six month old embryos and in adult tissues. An EGFP-ZNF425 fusion protein can be found in both the nucleus and the cytoplasm. ZNF425 appears to act as a transcription repressor. Over-expression of ZNF425 inhibits the transcriptional activities of SRE, AP-1, and SRF. Deletion analysis indicates that the C2H2 domain is the main region responsible for the repression. Our results suggest that the ZNF425 gene is a new transcriptional inhibitor that functions in the MAPK signaling pathway.
Keywords
Inhibitor; MAPK signaling pathway; Zinc finger protein; ZNF425;
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1 Herrera, R. E., Shaw, P. E. and Nordheim, A. (1989) Occupation of the c-fos serum response element by a multi-protein complex is unaltered by growth-factor induction. Nature 340, 68-70.   DOI   ScienceOn
2 Wang, Y., Luo, K., Li, Y., Zhu, C. and Wu, X. (2001) A rapid method of extracting total RNA from embryo tissues. Life Sci. Res. 5, 88-90.
3 Wang, Y., Zhou, J., Ye, X., Wan, Y., Li, Y., Mo, X., Yuan, W., Yan, Y., Luo, N., Wang, Z., Fan, X., Deng, Y. and Wu X. (2010) ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses NFAT and p21 pathway. BMB Rep. 43, 212-218.   DOI   ScienceOn
4 Shaknovich, R., Yeyati, P. L., Ivins, S., Melnick, A., Lempert, C., Waxman, S., Zelent, A., and Licht. J. D. (1998) The promyelocytic leukemia zinc finger protein affects myeloid cell growth, differentiation, and apoptosis. Mol. Cell. Biol. 18, 5533-5545.
5 Wu, X. (2006a) Introduction to Heart Development. Science Publishing House, Beijing, China
6 Wu, X. (2006b) Signal Pathways and Heart Development. Huaxuegongye Publishing House, Beijing, China
7 Whitmarsh, A. J. and Davis, R. J. (2000) Regulation of transcription factor function by phosphorylation. Cell. Mol. Life Sci. 45, 1172-1183.
8 Parlakian, A , Tuil, D., Hamard, G., Tavernier, G., Hentzen, D., Concordet, J. P., Paulin, D., Li, Z. and Daegelen, D. (2004) Targeted inactivation of serum response factor in the developing heart results in myocardial defects and embryonic lethality. Mol. Cell Biol. 24, 5281-5289.   DOI   ScienceOn
9 Kim, S. S., Chen, Y. M., Leary, E. O., Witzgall, R., Vidal, M. and Bonventre, J. V. (1996) A novel member of the RING finger family, KRIP-1, associated with the KRAB-A transcriptional repressor domain of zinc finger proteins. Proc. Natl. Acad. Sci. U.S.A. 93, 5299-15304.
10 Miano, J. M., Ramanan, N., Georger, M. A., de Nesy Bentley, K. L., Emerson, R. L., Balza, R. O., Jr Xiao, Q., Weiler, H., Ginty, D. and Misra, R. P. (2004) Restricted inactivation of serum response factor to the cardiovascular system. Proc. Nat. Acad. Sci. U.S.A. 101, 17132-17137.   DOI   ScienceOn
11 Luo, K., Wang, Y., Zhu, C., Li, Y., Wang, Y., Jiao, W., Liu, M. and Wu, X. (2002) Expression of a novel Kruppel-like zinc finger gene, ZNF382, in human heart. Biochem. Biophys. Res. Comm. 299, 606-612.   DOI   ScienceOn
12 Freire, G., Ocampo, C., Ilbawi, N., Griffin, A. J. and Gupta, M. (2007) Overt expression of AP-1 reduces alpha myosin heavy chain expression and contributes to heart failure from chronic volume overload. J. Mol. Cell. Cardiol. 43, 465-478.   DOI   ScienceOn
13 Margolin, J. F., Friedman, J. R., Meyer, W. K. H., Vissing, H., Thiesen, H. J. and Rauscher, F. J. (1994) Kruppel-associated boxes are potent transcriptional repression domains. Proc. Natl. Acad. Sci. U.S.A. 91, 4509-4513.   DOI
14 Bellefroid, E. J., Poncelet, D. A., Lecocq, P. J., Revelant, O. and Martial, J. A. (1991) The evolutionarily conserved Kruppel-associated box domain defines a subfamily of eukaryotic multifingered proteins. Proc. Natl. Acad. Sci. U.S.A. 88, 3608-3612.   DOI
15 Yi, F., Li, Y., Ma, W., Li, D., Zhu, C., Luo, J., Wang, Y., Huang, X., Yuan, W., Liu, M. and Wu, X. (2004) A novel KRAB zinc-finger protein, ZNF480, expresses in human heart and activates transcriptional activities of AP-1 and SRE. Biochem. Biophys. Res. Commun. 320, 409-415.   DOI   ScienceOn
16 Huang, X., Yuan, W., Huang, W., Bai, Y., Deng, Y., Zhu, C., Liang, P., Li, Y., Du, X., Liu. M., Wang, Y. and Wu, X. (2006) ZNF569, a novel KRAB-containing zinc finger protein, suppresses MAPK signaling pathway. Biochem. Biophys. Res. Commun. 346, 621-628.   DOI   ScienceOn
17 Li, Y., Yang, D., Bai, Y., Mo, X., Huang, W., Yuan, W., Yin, Z., Deng, Y., Murashko, O., Wang, Y., Fan, X., Zhu, C., Ocorr, K., Bodmer, R. and Wu, X. (2008) ZNF418, a novel human KRAB/C2H2 zinc finger protein, suppresses MAPK signaling pathway. Mol. Cell Biochem. 310, 141-151.   DOI
18 Brayer, K. J. and Segal, D. J. (2008) Keep your fingers off my DNA: protein-protein interactions mediated by C2H2 zinc finger domains. Cell Biochem. Biophys. 50, 111-131.   DOI   ScienceOn
19 Cheng, Y., Geng, H., Cheng, S., Liang, P., Bai, Y., Li, J., Srivastava, G., Ng, M., Fukagawa, T., Wu, X., Chan, T. C. and Tao, Q. (2010) The KRAB Zinc finger protein ZNF382 is a general, pro-apoptotic tumor suppressor repressing multiple oncogenes and frequently silenced by CpG methylation in multiple carcinomas. Cancer Res. 70, 6516-6526.   DOI
20 Klug, A. and Schwabe, J. W. R. (1995) Protein motifs 5. Zinc fingers. FASEB J. 9, 597-604.   DOI
21 Krishna, S. S., Majumdar, I. and Grishin, N. V. (2003) Structural classification of zinc fingers: survey and summary. Nucleic. Acids. Res. 31, 532-550.   DOI   ScienceOn
22 Lander, E. S., Linton, L. M., Birren, B., Nusbaum, C., Zody, M. C., Baldwin, J., Devon, K., Dewar, K. and Doyle, M. (2001) Initial sequencing and analysis of the human genome. Nature 409, 860-921.   DOI   ScienceOn
23 Nolte, R. T., Conlin, R. M., Harrison, S. C. and Brown, R. S. (1998) Differing roles for zinc finger in DNA recognition: structure of a six-finger transcription factor IIIA complex. Proc. Natl. Acad. Sci. 95, 2938-2943.   DOI
24 Cao, L., Wang, Z., Zhu, C., Zhao, Y., Yuan, W., Li, J., Wang, Y., Ying, Z., Li, Y., Yu, W., Wu, X. and Liu, M. (2005) ZNF383, a novel KRAB-containing zinc finger protein, suppresses MAPK signaling pathway. Biochem. Biophys. Res. Commun. 333, 1050-1059.   DOI   ScienceOn
25 Treisman, R. (1996) Regulation of transcription by MAP kinase cascades. Curr. Opin. Cell Biol. 8, 205-215.   DOI   ScienceOn
26 Looman, C., Abrink, M., Mark, C. and Hellman, L. (2002) KRAB zinc finger proteins: an analysis of the molecular mechanisms governing their increase in numbers an complexity during evolution. Mol. Biol. Evol. 19, 2118-2130.   DOI   ScienceOn
27 Nancy, F., Qian, Z., Kathleen, G., Cheol, Y. C., Yongsok, K. and Robert, A. S. (2000) The multitype zinc-finger protein U-shaped functions in heart cell specification in the Drosophila embryo. Proc. Natl. Acad. Sci. U.S.A. 97, 7348-7353.   DOI
28 Chang, L. and Karin, M. (2001) Mammalian MAP kinase signaling cascades. Nature 410, 37-40.   DOI   ScienceOn