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http://dx.doi.org/10.5483/BMBRep.2011.44.12.787

Effect of silk fibroin peptide derived from silkworm Bombyx mori on the anti-inflammatory effect of Tat-SOD in a mice edema model  

Kim, Dae-Won (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Hwang, Hyun-Sook (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Kim, Duk-Soo (Department of Anatomy, College of Medicine, Soonchunhyang University)
Sheen, Seung-Hoon (Department of Neurosurgery, Hallym University Medical Center)
Heo, Dong-Hwa (Department of Neurosurgery, Hallym University Medical Center)
Hwang, Gyo-Jun (Department of Neurosurgery, Hallym University Medical Center)
Kang, Suk-Hyung (Department of Neurosurgery, Hallym University Medical Center)
Kweon, Hae-Yong (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA)
Jo, You-Young (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA)
Kang, Seok-Woo (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA)
Lee, Kwang-Gill (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA)
Park, Kye-Won (Department of Food Science and Biotechnology, Sungkyunkwan University)
Han, Kyu-Hyung (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Park, Jin-Seu (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Eum, Won-Sik (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Cho, Yong-Jun (Department of Neurosurgery, Hallym University Medical Center)
Choi, Hyun-Chul (Department of Neurosurgery, Hallym University Medical Center)
Choi, Soo-Young (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Publication Information
BMB Reports / v.44, no.12, 2011 , pp. 787-792 More about this Journal
Abstract
We investigated whether silk fibroin peptide derived from the silkworm, Bombyx mori, could inhibit inflammation and enhance the anti-inflammatory activity of Tat-superoxide dismutase (Tat-SOD), which was previously reported to effectively penetrate various cells and tissues and exert anti-oxidative activity in a mouse model of inflammation. Inflammation was induced by topical treatment of mouse ears with 12-O-tetradecanoylphorbol-13-acetate (TPA). Histological, Western blot, and reverse transcription-polymerase chain reaction data demonstrated that silk fibroin peptide or Tat-SOD alone could suppress elevated levels of cyclooxygenase-2, interleukin-6, interleukin-1beta, and tumor necrosis factor-alpha induced by TPA. Moreover, silk fibroin peptide significantly enhanced the anti-inflammatory activity of Tat-SOD, although it had no influence on in vitro and in vivo transduction of Tat-SOD. Silk fibroin peptide exhibited anti-inflammatory activity in a mice model of inflammation. Therefore, silk fibroin peptide alone or in combination with Tat-SOD might be used as a therapeutic agent for various inflammatory diseases.
Keywords
Inflammation; Protein therapy; Silk fibroin; Tat-superoxide dismutase; TPA;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
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