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http://dx.doi.org/10.5483/BMBRep.2011.44.12.782

p53 is not necessary for nuclear translocation of GAPDH during NO-induced apoptosis  

Kim, Jum-Ji (Department of Medical Biotechnology, Soonchunhyang University)
Lee, Mi-Young (Department of Medical Biotechnology, Soonchunhyang University)
Publication Information
BMB Reports / v.44, no.12, 2011 , pp. 782-786 More about this Journal
Abstract
Aberrant GAPDH expression following S-nitrosoglutathione (GSNO) treatment was compared in HepG2 cells, which express functional p53, and Hep3B cells, which lack functional p53. The results of Western blotting and fluorescent immunocytochemistry revealed that nuclear translocation and accumulation of GAPDH occur in both HepG2 and Hep3B cells. This finding suggests that p53 may not be necessary for the GSNO-induced translocation of GAPDH to the nucleus during apoptotic cell death in hepatoma cells.
Keywords
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH); Hepatoma cells; Nuclear translocation; p53; S-nitrosoglutathione (GSNO);
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1 Lee, K. H., Kim, K. C., Jung, Y. J., Ham, Y. H., Jang, J. J., Kwon, H., Sung, Y. C., Kim, S. H., Han, S. K. and Kim, C. M. (2001) Induction of apoptosis in p53-deficient human hepatoma cell line by wild-type p53 gene transduction: inhibition by antioxidant. Mol. Cells 12, 17-24.
2 Jeon, Y. M., Son, B. S. and Lee, M. Y. (2011) Proteomic identification of the differentially expressed proteins in human lung epithelial cells by airborne particulate matter. J. Appl. Toxicol. 31, 45-52.   DOI   ScienceOn
3 Yego, E. C. and Mohr, S. (2010) siah-1 Protein is necessary for high glucose-induced glyceraldehyde-3-phosphate dehydrogenase nuclear accumulation and cell death in Muller cells. J. Biol. Chem. 285, 3181-3190.   DOI   ScienceOn
4 Ventura, M., Mateo, F., Serratosa, J., Salaet, I., Carujo, S., Bachs, O. and Pujol, M. J. (2010) Nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase is regulated by acetylation. Int. J. Biochem. Cell Biol. 42, 1672-1680.   DOI   ScienceOn
5 Sen, N., Hara, M. R., Kornberg, M. D., Cascio, M. B., Bae, B. I., Shahani, N., Thomas, B., Dawson, T. M., Dawson, V. L., Snyder, S. H. and Sawa, A. (2008) Nitric oxide-induced nuclear GAPDH activates p300/CBP and mediates apoptosis. Nat. Cell Biol. 10, 866-873.   DOI   ScienceOn
6 Huang, J., Hao, L., Xiong, N., Cao, X., Liang, Z., Sun, S. and Wang, T. (2009) Involvement of glyceraldehydes-3-phosphate dehydrogenase in rotenone induced-cell apoptosis: relevance to protein misfolding and aggregation. Brain Res. 1279, 1-8.   DOI   ScienceOn
7 Nakajima, H., Amano, W., Fujita, A., Fukuhara, A., Azuma, Y. T., Hata, F., Inui, T. and Takeuchi, T. (2007) The active site cysteine of the proapoptotic protein glyceraldehydes-3-phosphate dehydrogenase is essential in oxidative stress-induced aggregation and cell death. J. Biol. Chem. 282, 26562-26574.   DOI   ScienceOn
8 Hara, M. R. and Snyder, S. H. (2006) Nitric oxide-GAPDH-Siah: a novel cell death cascade. Cell Mol. Neurobiol. 26, 527-538.
9 Messmer, U. K. and Brune, B. (1996) Nitric oxide-induced apoptosis: p53-dependent and p53-independent signalling pathways. Biochem. J. 319, 299-305.   DOI
10 Kibbe, M. R., Li, J., Nie, S., Choi, B. M., Kovesdi, I., Lizonova, A., Billiar, T. R. and Tzeng, E. (2002) Potentiation of nitric oxide-induced apoptosis in p53-/- vascular smooth muscle cells. Am. J. Physiol. Cell Physiol. 282, C625-C634.   DOI   ScienceOn
11 Han, L. L., Xie, L. P., Li, L. H., Zhang, X. W., Zhang, R. Q. and Wang, H. Z. (2009) Reactive oxygen species production and Bax/Bcl-2 regulation in honokiol-induced apoptosis in human hepatocellular carcinoma SMMC-7721 cells. Environ. Toxicol. Pharmacol. 28, 97-103.   DOI   ScienceOn
12 Kim, J. J., Kim, Y. H. and Lee, M. Y. (2009) Proteomic characterization of differentially expressed proteins associated with nitric oxide stress in retinal ganglion cells. BMB Rep. 42, 456-461.   DOI   ScienceOn
13 Kim, J. J. and Lee, M. Y. (2009) Differential expression of cytosolic and nuclear proteins during S-nitrosoglutathione-induced cell death. Biochip J. 3, 326-332.
14 Sirover, M. A. (1997) Role of the glycolytic protein, glyceraldehydes- 3-phosphate dehydrogenase, in normal cell function and in cell pathology. J. Cell Biochem. 66, 133-140.   DOI   ScienceOn
15 Vaudry, D. Falluel-Morel, A., Leuillet, S., Vaudry, H. and Gonzalez, B. J. (2003) Regulators of cerebellar granule cell development act through specific signaling pathways. Science 300, 1532-1534.   DOI   ScienceOn
16 Sirover, M. A. (2005) New nuclear functions of the glycolytic protein, glyceraldehydes-3-phosphate dehydrogenase, in mammalian cells. J. Cell Biochem. 95, 45-52.   DOI   ScienceOn
17 Senatorov, V. V., Charles, V., Reddy, P. H., Tagle, D. A. and Chuang, D. M. (2003) Overexpression and nuclear accumulation of glyceraldehydes-3-phosphate dehydrogenase in transgenic mouse model of Huntington's disease. Mol. Cell Neurosci. 22, 285-297.   DOI   ScienceOn
18 Mazzola, J. L. and Sirover, M. A. (2003) Subcellular localization of human glyceraldehydes-3-phosphate dehydrogenase is independent of its glycolytic function. Biochim. Biophys. Acta. 1622, 50-56.   DOI   ScienceOn
19 Berry, M. D. and Boulton, A. A. (2000) Glyceraldehyde-3-phosphate dehydrogenase and apoptosis. J. Neurosci. Res. 60, 150-154.   DOI   ScienceOn
20 Butterfield, D. A., Hardas, S. S. and Lange, M. L. (2010) Oxidatively modified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Alzheimer's disease: many pathways to neurodegeneration. J. Alzheimers Dis. 20, 369-393.   DOI
21 Moncada, S., Palmer, R. M. and Higgs, E. A. (1991) Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol. Rev. 43, 109-142.
22 Nathan, C. (1992) Nitric oxide as a secretory product of mammalian cell. FASEB J. 6, 3051-3064.   DOI
23 Mohr, S., Hallak, H., de Boitte, A., Lapetina, E. G. and Brune, B. (1999) Nitric oxide-induced S-glutathionylation and inactivation of glyceraldehyde-3-phosphate dehydrogenase. J. Biol. Chem. 274, 9427-9430.   DOI   ScienceOn
24 Messmer, U. K., Ankarcrona, M., Nicotera, P. and Brune, B. (1994) p53 expression in nitric oxide-induced apoptosis. FEBS Lett. 355, 23-26.   DOI   ScienceOn
25 Kim, C. I., Lee, S. H., Seong, G. J., Kim, Y. H. and Lee, M. Y. (2006) Nuclear translocation and overexpression of GAPDH by the hyper-pressure in retinal ganglion cell. Biochem. Biophys. Res. Commun. 341, 1237-1243.   DOI   ScienceOn
26 Lowe, S. W., Schmitt, E. M., Smith, S. W., Osborne, B. A. and Jacks, T. (1993) p53 required for radiation-induced apoptosis in mouse thymocyte. Nature 362, 847-849.   DOI   ScienceOn
27 Clarke, A. R., Purdie, C. A., Harrison, D. J., Morris, R. G., Bird, C. C., Hooper, M. L. and Wyllie, A. H. (1993) Thymocyte apoptosis induced by p53-dependent and independent pathways. Nature 362, 849-852.   DOI   ScienceOn
28 Ho, Y. S., Liu, H. L., Duh, J. S., Chen, R. J., Ho, W. L., Jeng, J. H., Wang, Y. J. and Lin, J. K. (1999) Induction of apoptosis by S-nitrosoglutathione and $Cu^{2+}\;or\;Ni^{2+}$ ion through modulation of bax, bad, and bcl-2 proteins in human colon adenocarcinoma cells. Mol. Carcinog. 26, 201-211.   DOI   ScienceOn