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http://dx.doi.org/10.5483/BMBRep.2010.43.8.573

Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans  

Lang, Guo-Jun (Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, and Institute of Insect Science, Zhejiang University)
Zhang, Ming-Yan (Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, and Institute of Insect Science, Zhejiang University)
Li, Bao-Ling (Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, and Institute of Insect Science, Zhejiang University)
Yu, Lin-Lin (Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, and Institute of Insect Science, Zhejiang University)
Lu, Xing-Meng (Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, and Institute of Insect Science, Zhejiang University)
Zhang, Chuan-Xi (Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, and Institute of Insect Science, Zhejiang University)
Publication Information
BMB Reports / v.43, no.8, 2010 , pp. 573-578 More about this Journal
Abstract
Several organophosphorus (OP) insecticides can selectively kill the silkworm maggot, Exorista sorbillans (Es) (Diptera: Tachinidae), while not obviously affecting the host (Bombyx mori) larvae, but the mechanism is not yet clear. In this study, the cDNA encoding an acetylcholinesterase (AChE) from the field Es was isolated. One point mutation (Gly353Ala) was identified. The Es-353G AChE and Es-353A AChE were expressed in baculovirus-insect cell system, respectively. The inhibition results showed that for eserine and Chlorpyrifos, Es-353A AChE was significantly less sensitive than Es-353G AChE. Meanwhile, comparison of the I50 values of eserine, dichlorvos, Chlorpyrifos and omethoate of recombinant Es AChEs with its host (Bombyx mori) AChEs indicated that, both Es AChEs are more sensitive than B. mori AChEs. The results give an insight of the mechanism that some OP insecticides can selectively kills Es while without distinct effect on its host, B. mori.
Keywords
Acetylcholinesterase sequence; Exorista sorbillans; Gene expression; Inhibition comparison; Point mutation;
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1 Haas, R., Marshall, T. L. and Rosenberry, T. L. (1988) Drosophila acetylcholinesterase: demonstration of a glycoinositol phospholipid anchor and an endogenous proteolytic cleavage. Biochemistry 27, 6453-6457.   DOI   ScienceOn
2 Lang, G. J., Shang, J. Y., Chen, Y. X., Cui, Y. J., Wang, Q., Tang, Z. H. and Zhang, C. X. (2010) Expression of the housefly acetylcholinesterase in a bioreactor and its potential application in the detection of pesticide residues. World J. Microbiol. Biotechnol. DOI 10.1007/s11274-010-0360-4.
3 Ellman, G. L., Courtney, K. D., Andres, V. and Featherstone, R. M. (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7, 88-95   DOI   ScienceOn
4 Tang, Q. and Feng, M. (2002) $DPS^{\copyright}$ Data Processing System for Practical Statistics, Science Press, Peking, China.
5 Zhu, K. Y., Lee, S. H. and Clark, J. M. (1996) A point mutation of AChE associated with azinphosmethyl resistance and reduced fitness in Colorado potato beetle. Pest. Biochem. Physiol. 55, 100-108.   DOI   ScienceOn
6 Aldridge, W. N. and Reiner, E. (1972) Enzyme inhibitors as substrates: interactions of esterases with esters of organophosphorus and carbamic acids. In Frontiers of Biology 26, 41-44.
7 Main, A. R. (1964) Affinity and phosphorylation constants for the inhibition of esterases by OPs. Science 144, 992-993.   DOI   ScienceOn
8 Vontas, J. G., Hejazi, M. J., Hawkes, N. J., Cosmidis, N., Loukas, M., Janes, R. W. and Hemingway, J. (2002) Resistance-associated point mutations of organophosphate insensitive acetylcholinesterase, in the olive fruit fly Bactrocera oleae. Insect Mol. Biol. 11, 329-336   DOI   ScienceOn
9 Jin, W., Lu, X. M., Wan, Y. J., Gong, C. L. and Liao, F. P. (2001) Pathematology of silkworm. Hang zhou: Zhe jiang Agriculture press.
10 Chen, Z., Newcomb, R., Forbes, E., McKenzie, J. and Batterham, P. (2001) The acetylcholinesterase gene and organophosphorus resistance in the Australian sheep blowfly, Lucilia cuprina. Insect Biochem Mol. Biol. 31, 805-816.   DOI   ScienceOn
11 Lee, D. W., Choi, J. Y., Kim, W. T., Yeon, H. J., Song, J. T., Chung, B. K., Boo, K. S. and Koh, Y. H. (2007) Mutations of acetylcholinesterase1 contribute to prothiofosresistance in Plutella xylostella. Biochemical and Biophysical Research Communications 353, 591-597.   DOI   ScienceOn
12 Harel, M., Kryger, G., Rosenberry, T. L., Mallender, W. D., Lewis, T., Fletcher, R. J., Guss, J. M., Silman, I. and Sussman, J. L. (2000) Three-dimensional structures of Drosophila melanogaster AChEand of and of its complexes with two potent inhibitors. Protein Sci. 9, 1063-1072.   DOI   ScienceOn
13 Mutero, A., Pralavorio, M., Bride, J. M. and Fournier, D. (1994) Resistance-associated point mutation in insecticideinsensitive acetylcholinesterase. Proc. Natl. Acad. Sci. 91, 5922-5926   DOI   ScienceOn
14 Fournier, D. and Mutero, A. (1994) Modification of AChE as a mechanism of resistance to insecticides. Comp. Biochem. Physiol. 108C, 19-31.
15 Walsh, S. B., Dolden, T. A., Moores, G. D., Kristensen, M., Lewis, T., Devonshire, A. L. and Williamson, M. S. (2001) Identification and characterization of mutation in the housefiy (Musca domestica) AChEinvolved in insecticide resistance. Biochem. J. 359, 175-181.   DOI
16 Fournier, D. (2005) Mutations of acetylcholinesterase which confer insecticide resistance in insect populations. Chemico-Biological Interactions 157, 257-261.   DOI   ScienceOn
17 Taylor, P. (1990) The pharmacological basis of therapeutics, 8th ed., MacMillan, New York, USA.
18 Hall, L. M. and Spierer, P. (1986) The ace locus of Drosophila melanogaster: structural gene for AChE with an unusual 5’ leader, EMBO J. 5, 2949-2954.
19 Bourguet, D., Raymond, M., Fournier, D., Malcolm, C. A., Toutant, J. P. and Arpagaus, M. (1996) Existence of two acetylcholinesterases in the mosquito Culex pipiens (Diptera: Culicidae). J. Neurochem. 67, 2115-2123.   DOI
20 Ono, Y. and Tomita, T. (2006) Amino acid substitutions conferring insecticide insensitivity in Ace-paralogous acetylcholinesterase. Pesticide Biochemistry and Physiology 85, 123-132.   DOI   ScienceOn
21 Shang, J. Y., Shao, Y. M., Lang, G. J., Zhang, C. X., Yuan, G. and Tang, Z. H. (2007) Expression of two types of acetylcholinesterase gene from the silkworm, Bombyx mori, in insect cells. Insect Science 6, 443-449.
22 Gao, J. R., Rao, J. V., Wilde, G. E. and Zhu, K. Y. (1998) Purification and Kinetic Analysis of Acetylcholinesterase From Western Corn Rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). Archives of Insect Biochemistry and Physiology 39, 118-125.   DOI   ScienceOn