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http://dx.doi.org/10.5483/BMBRep.2010.43.2.097

Pig large tumor suppressor 2 (Lats2), a novel gene that may regulate the fat reduction in adipocyte  

Liu, Qiuyue (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Gu, Xiaorong (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Zhao, Yiqiang (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Zhang, Jin (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Zhao, Yaofeng (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Meng, Qingyong (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Xu, Guoheng (Peking University Health Science Center)
Hu, Xiaoxiang (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Li, Ning (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Publication Information
BMB Reports / v.43, no.2, 2010 , pp. 97-102 More about this Journal
Abstract
Clenbuterol, a $\beta_2$-adrenoceptor agonist, has been proven to be a powerful repartition agent that can decrease fat deposition. Based on results from our previous cDNA microarray experiment of pig clenbuterol administration, a novel up-regulated EST was full-length cloned (4859 bp encoding 1041 amino acids) and found to be the pig homolog of large tumor suppressor 2 (Lats2). We mapped pig Lats2 to chromosome 11p13-14 by using FISH, and western blotting demonstrated that pig Lats2 protein was most abundant in adipose. In Drosophila, Lats2 ortholog was reported as a key component of the Hippo pathway which regulates cell differentiation and growth. Here, we show that pig Lats2 exhibit inverted expression to YAP1, another member of the Hippo pathway which positively regulates cell growth and proliferation, during the differentiation of 3T3-L1 preadipocytes. Our results suggested that Lats2 may involve in Hippo pathway regulating the fat reduction by inhibiting adipocyte differentiation and growth.
Keywords
Adipose tissue; $\beta_2$-adrenoceptor agonist; Clenbuterol; Hippo pathway; Lats2; Pig;
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