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http://dx.doi.org/10.5483/BMBRep.2010.43.2.140

High glucose induces differentiation and adipogenesis in porcine muscle satellite cells via mTOR  

Yue, Tao (State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University)
Yin, Jingdong (State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University)
Li, Fengna (State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University)
Li, Defa (State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University)
Du, Min (Department of Animal Science, University of Wyoming)
Publication Information
BMB Reports / v.43, no.2, 2010 , pp. 140-145 More about this Journal
Abstract
The present study investigated whether the mammalian target of rapamycin (mTOR) signal pathway is involved in the regulation of high glucose-induced intramuscular adipogenesis in porcine muscle satellite cells. High glucose (25 mM) dramatically increased intracellular lipid accumulation in cells during the 10-day adipogenic differentiation period. The expressions of CCAAT/enhancer binding protein-$\alpha$ (C/EBP-$\alpha$) and fatty acid synthase (FAS) protein were gradually enhanced during the 10-day duration while mTOR phosphorylation and sterol-regulatory- element-binding protein (SREBP)-1c protein were induced on day 4. Moreover, inhibition of mTOR activity by rapamycin resulted in a reduction of SREBP-1c protein expression and adipogenesis in cells. Collectively, our findings suggest that the adipogenic differentiation of porcine muscle satellite cells and a succeeding extensive adipogenesis, which is triggered by high glucose, is initiated by the mTOR signal pathway through the activation of SREBP-1c protein. This process is previously uncharacterized and suggests a cellular mechanism may be involved in ectopic lipid deposition in skeletal muscle during type 2 diabetes.
Keywords
Adipocytes; CCAAT/enhancer binding protein-$\alpha$; Lipid accumulation; mTOR signal pathway; Sterol-regulatory-elementbinding protein-1c; Type 2 diabetes;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
Times Cited By SCOPUS : 9
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