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http://dx.doi.org/10.5483/BMBRep.2010.43.2.127

PI(3,4,5)P3 regulates the interaction between Akt and B23 in the nucleus  

Kwon, Il-Sun (Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)
Lee, Kyung-Hoon (Department of Anatomy, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)
Choi, Joung-Woo (Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)
Ahn, Jee-Yin (Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)
Publication Information
BMB Reports / v.43, no.2, 2010 , pp. 127-132 More about this Journal
Abstract
Phosphatidylinositol (3,4,5)-triphosphate ($PIP_3$) is a lipid second messenger that employs a wide range of downstream effector proteins for the regulation of cellular processes, including cell survival, polarization and proliferation. One of the most well characterized cytoplasmic targets of $PIP_3$, serine/threonine protein kinase B (PKB)/Akt, promotes cell survival by directly interacting with nucleophosmin (NPM)/B23, the nuclear target of $PIP_3$. Here, we report that nuclear $PIP_3$ competes with Akt to preferentially bind B23 in the nucleoplasm. Mutation of Arg23 and Arg25 in the PH domain of Akt prevents binding to $PIP_3$, but does not disrupt the Akt/B23 interaction. However, treatment with phosphatases PTEN or SHIP abrogates the association between Akt and B23, indicating that nuclear $PIP_3$ regulates the Akt/B23 interaction by controlling the concentration and subcellular dynamics of these two proteins.
Keywords
Akt; B23; Neuronal cell; Nuclear PIP3; Subcellular dynamics;
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