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http://dx.doi.org/10.5483/BMBRep.2010.43.1.046

Protection of burn-induced skin injuries by the flavonoid kaempferol  

Park, Byoung-Kwon (Department of Microbiology, College of Medicine, Hallym University)
Lee, Soo-Hyoung (Department of Microbiology, College of Medicine, Hallym University)
Seo, Jae-Nam (Department of Pathology, College of Medicine, Hallym University)
Rhee, Jae-Won (Center for Medical Science Research, Hallym University)
Park, Jae-Bong (Department of Biochemistry, College of Medicine, Hallym University)
Kim, Yong-Sun (Department of Microbiology, College of Medicine, Hallym University)
Choi, Ihn-Geun (Department of Neuropsychiatry, Hallym University)
Kim, Young-Eun (Department of Biochemistry, College of Natural Sciences, Chungbuk National University)
Lee, Young-Hee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University)
Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University)
Publication Information
BMB Reports / v.43, no.1, 2010 , pp. 46-51 More about this Journal
Abstract
Thermal burn injury induces inflammatory cell infiltrates in the dermis and thickening of the epidermis. Following a burn injury, various mediators, including reactive oxygen species (ROS), are produced in macrophages and neutrophils, exposing all tissues to oxidative injury. The anti-oxidant activities of flavonoids have been widely exploited to scavenge ROS. In this study, we observed that several flavonoids-kaempferol, quercetin, fisetin, and chrysin-inhibit LPS-induced IL-8 promoter activation in RAW 264.7 cells. In contrast with quercetin and fisetin, pretreatment of kaempferol and chrysin did not decrease cell viability. Inflammatory cell infiltrates in the dermis and thickening of the epidermis induced by burn injuries in mice was relieved by kaempferol treatment. However, the injury was worsened by fisetin, quercetin, and chrysin. Expression of TNF-a induced by burn injuries was decreased by kaempferol. These findings suggest the potential use of kaempferol as a therapeutic in thermal burn-induced skin injuries.
Keywords
Burn; Flavonoids; Kaempferol; TNF-$\alpha$;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 7  (Related Records In Web of Science)
Times Cited By SCOPUS : 7
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1 Gibran, N. S., Ferguson, M., Heimbach, D. M. and Isik, F.F. (1997) Monocyte chemoattractant protein-1 mRNA expression in the human burn wound. J. Surg. Res. 70, 1-6   DOI   ScienceOn
2 Kempuraj, D., Madhappan, B., Christodoulou, S., Boucher, W.,Cao, J., Papadopoulou, N., Cetrulo, C. L. and Theoharides, T. C. (2005) Flavonols inhibit proinflammatory mediator release, intracellular calcium ion levels and protein kinase C theta phosphorylation in human mast cells. Br. J. Pharmacol. 145, 934-944   DOI   ScienceOn
3 Comalada, M., Ballester, I., Bail\acute{o}n, E., Sierra, S., Xaus, J.,G\acute{a}lvez, J., de Medina, F. S. and Zarzuelo, A. (2006) Inhibition of pro-inflammatory markers in primary bone marrow-derived mouse macrophages by naturally occurring flavonoids:analysis of the structure-activity relationship. Biochem. Pharmacol. 72, 1010-1021   DOI   ScienceOn
4 Lee, S., Kim, Y. J., Kwon, S., Lee, Y., Choi, S. Y., Park, J. and Kwon, H. J. (2009) Inhibitory effects of flavonoids on TNF-α-induced IL-8 gene expression in HEK 293 cells. BMB Rep. 42, 265-270   DOI   PUBMED   ScienceOn
5 Samhan-Arias, A. K., Martín-Romero, F. J. and Gutiérrez-Merino, C. (2004) Kaempferol blocks oxidative stress in cerebellar granule cells and reveals a key role for reactive oxygen species production at the plasma membrane in the commitment to apoptosis. Free Radic. Biol. Med. 37, 48-61   DOI   ScienceOn
6 Moss, N. M., Gough, D. B., Jordan, A. L., Grbic, J. T.,Wood, J. J., Rodrick, M. L. and Mannick, J. A. (1988) Temporal correlation of impaired immune response after thermal injury with susceptibility to infection in a murine model. Surgery 104, 882-887   PUBMED
7 Pruitt, B. A. Jr. (1990) Infection and the burn patient. Br. J. Surg. 77, 1081-1082   DOI   ScienceOn
8 Schwacha, M. G. (2003) Macrophages and post-burn immune dysfunction. Burns 29, 1-14   DOI   PUBMED   ScienceOn
9 Dipietro, L. A., Reintjes, M. G., Low, Q. E., Levi, B. and Gamelli, R. L. (2001) Modulation of macrophage recruitment into wounds by monocyte chemoattractant protein-1. Wound Repair Regen. 9, 28-33   DOI   ScienceOn
10 Drost, A. C., Burleson, D. G., Cioffi, W. G. Jr., Mason, A.D. Jr. and Pruitt, B. A. Jr. (1993) Plasma cytokines after thermal injury and their relationship to infection. Ann. Surg. 218, 74-78   DOI   ScienceOn
11 Walker, H.L. and Mason, A.D. Jr. (1968) A standard animal burn. J. Trauma 8, 1049-1051   DOI   PUBMED
12 Parihar, A., Parihar, M. S., Milner, S. and Bhat, S. (2008)Oxidative stress and anti-oxidative mobilization in burn injury. Burns 34, 6-17   DOI   ScienceOn
13 O'Riordain, M. G., Collins, K. H., Pilz, M., Saporoschetz,I. B., Mannick, J. A. and Rodrick, M. L. (1992) Modulation of macrophage hyperactivity improves survival in a burn-sepsis model. Arch. Surg. 127, 152-158   DOI   PUBMED   ScienceOn
14 Hu, M. (2007) Commentary: bioavailability of flavonoids and polyphenols: call to arms. Mol. Pharm. 4, 803-806   DOI   PUBMED   ScienceOn
15 Faunce, D. E., Llanas, J. N. Patel, P. J., Gregory, M. S.,Duffner, L. A. and Kovacs, E. J. (1999) Neutrophil chemokine production in the skin following scald injury. Burns 25, 403-410   DOI   ScienceOn
16 Pang, J. L., Ricupero, D. A., Huang, S., Fatma, N., Singh, D.P., Romero, J. R. and Chattopadhyay, N. (2006) Differential activity of kaempferol and quercetin in attenuating tumor necrosis factor receptor family signaling in bone cells. Biochem. Pharmacol. 71, 818-826   DOI   ScienceOn
17 O'Sullivan, S. T. and O'Connor, T. P. (1997) Immunosuppression following thermal injury: the pathogenesis of immunodysfunction. Br. J. Plast. Surg. 50, 615-623   DOI   ScienceOn
18 O'Sullivan, S. T., Lederer, J. A., Horgan, A. F., Chin, D.H., Mannick, J. A. and Rodrick, M. L. (1995) Major injury leads to predominance of the T helper-2 lymphocyte phenotype and diminished interleukin-12 production associated with decreased resistance to infection. Ann. Surg. 222, 482-492   DOI   PUBMED
19 Rawlingson, A. (2003) Nitric oxide, inflammation and acute burn injury. Burns 29, 631-640   DOI   PUBMED   ScienceOn
20 Meakins, J. L. (1990) Etiology of multiple organ failure. J. Trauma 30, S165-168   DOI   PUBMED
21 Toklu, H. Z., Tunali-Akbay, T., Erkanli, G., Yuksel, M.,Ercan, F. and Sener, G. (2007) Silymarin, the antioxidant component of Silybum marianum, protects against burninduced oxidative skin injury. Burns 33, 908-916   DOI   ScienceOn
22 Beukelman, C. J., van den Berg, A. J., Hoekstra, M. J., Uhl, R., Reimer, K. and Mueller, S. (2008) Anti-inflammatory properties of a liposomal hydrogel with povidone-iodine (Repithel) for wound healing in vitro. Burns 34, 845-855   DOI   ScienceOn
23 Teodorczyk-Injeyan, J. A., Sparkes, B. G., Mills, G. B., Peters, W. J. and Falk, R. E. (1986) Impairment of T cell activation in burn patients: a possible mechanism of thermal injury induced immunosuppression. Clin. Exp. Immunol. 65, 570-581   PUBMED
24 Luo, G., Peng, D., Zheng, J., Chen, X., Wu, J., Elster, E. and Tadaki, D. (2005) The role of NO in macrophage dysfunction at early stage after burn injury. Burns 31, 138-144   DOI   ScienceOn
25 Shallo, H., Plackett, T. P., Heinrich, S. A. and Kovacs, E. J.(2003) Monocyte chemoattractant protein-1 (MCP-1) and macrophage infiltration into the skin after burn injury in aged mice. Burns 29, 641-647   DOI   ScienceOn
26 Sayeed, M. M. (1996) Alterations in cell signaling and related effector functions in T lymphocytes in burn/trauma/septic injuries. Shock 5, 157-166   DOI   PUBMED
27 Park, H. H., Lee, S., Son, H. Y , Park, S. B., Kim, M. S.,Choi, E. J., Singh, T. S., Ha, J. H., Lee, M. G., Kim, J. E.,Hyun, M. C., Kwon, T. K., Kim, Y. H. and Kim, S. H. (2008)Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells. Arch. Pharm. Res. 31, 1303-1311   DOI   ScienceOn
28 Kim, Y. J., Kim, D., Lee, Y., Choi, S. Y., Park, J., Lee, S. Y.,Park, J. W. and Kwon, H. J. (2009) Effects of nanoparticulate saponin-platinum conjugates on 2,4-dinitrofluorobenzeneinduced macrophage inflammatory protein-2 gene expression via reactive oxygen species production in RAW 264.7 cells. BMB rep. 42, 304-309   DOI   PUBMED   ScienceOn
29 Park, J. S., Rho, H. S., Kim, D. H. and Chang, I. S. (2006)Enzymatic preparation of kaempferol from green tea seed and its antioxidant activity. J. Agric. Food Chem. 54, 2951-2956   DOI   ScienceOn
30 Armstrong, D. A., Major, J. A., Chudyk, A. and Hamilton, T. A. (2004) Neutrophil chemoattractant genes KC and MIP-2 are expressed in different cell populations at sites of surgical injury. J. Leukoc. Biol. 75, 641-648   DOI   ScienceOn