[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.2206.06024

Functional Roles of Exosomes in Allergic Contact Dermatitis

Bocui Song (Department of Pharmaceutical Engineering, College of Life Science and Technology, Heilongjiang Bayi Agricultural University)
Qian Chen (Molecular Mechanism of Disease and Research and Development of Bioactive Substances, College of Life Science and Technology, Heilongjiang Bayi Agricultural University)
Yuqi Li (Molecular Mechanism of Disease and Research and Development of Bioactive Substances, College of Life Science and Technology, Heilongjiang Bayi Agricultural University)
Shuang Zhan (Animal Husbandry and Veterinary Station of Yongji Economic Development Zone)
Rui Zhao (Department of Pharmaceutical Engineering, College of Life Science and Technology, Heilongjiang Bayi Agricultural University)
Xue Shen (Molecular Mechanism of Disease and Research and Development of Bioactive Substances, College of Life Science and Technology, Heilongjiang Bayi Agricultural University)
Min Liu (Department of Pharmaceutical Engineering, College of Life Science and Technology, Heilongjiang Bayi Agricultural University)
Chunyu Tong (Department of Biological Science, College of Life Science and Technology, Heilongjiang Bayi Agricultural University)

Publication Information

Journal of Microbiology and Biotechnology / v.32, no.12, 2022 , pp. 1506-1514 More about this Journal

Abstract

Allergic contact dermatitis (ACD) is an allergen-specific T-cell-mediated inflammatory response, albeit with unclear pathogenesis. Exosomes are nanoscale extracellular vesicles secreted by several cell types and widely distributed in various biological fluids. Exosomes affect the occurrence and development of ACD through immunoregulation among other ways. Nevertheless, the role of exosomes in ACD warrants further clarification. This review examines the progress of research into exosomes and their involvement in the pathogenesis, diagnosis, and treatment of ACD and provides ideas for exploring new diagnostic and treatment methods for this disease.

Keywords

Allergic contact dermatitis; exosomes; immunoregulation; inflammation;

Citations & Related Records

Times Cited By KSCI : 8 (Citation Analysis)

Reference
Cited By KSCI

1	van Eijndhoven MAJ, Zijlstra JM, Groenewegen NJ, Drees EEE, van Niele S, Baglio SR, et al. 2016. Plasma vesicle miRNAs for therapy response monitoring in Hodgkin lymphoma patients. JCI Insight 1: e89631.
2	Monguio-Tortajada M, Galvez-Monton C, Bayes-Genis A, Roura S, Borras FE. 2019. Extracellular vesicle isolation methods: rising impact of size-exclusion chromatography. Cell Mol. Life Sci. 76: 2369-2382. DOI
3	Monguio-Tortajada M, Prat-Vidal C, Moron-Font M, Clos-Sansalvador M, Calle A, Gastelurrutia P, et al. 2021. Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction. Bioact. Mater. 6: 3314-3327. DOI
4	Monguio-Tortajada M, Moron-Font M, Gamez-Valero A, Carreras-Planella L, Borras FE, Franquesa M, et al. 2019. Extracellular-vesicle isolation from different biological fluids by size-exclusion chromatography. Curr. Protoc. Stem Cell Biol. 49: e82.
5	Sitar S, Kej ar A, Pahovnik D, Kogej K, Tusek-Znidaric M, Lenass M, et al. 2015. Size characterization and quantification of exosomes by asymmetrical-flow field-flow fractionation. Anal. Chem. 87: 9225-9233. DOI
6	Kang D, Oh S, Ahn S M, Lee BH, Moon MH. 2008. Proteomic analysis of exosomes from human neural stem cells by flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry. J. Proteome Res. 7: 3475-3480. DOI
7	Weng Y, Sui Z, Shan Y, Hu YC, Chen YuB, Zhang LiH, et al. 2016. Effective isolation of exosomes with polyethylene glycol from cell culture supernatant for in-depth proteome profiling. Analyst. 141: 4640-4646. DOI
8	Rider MA, Hurwitz SN, Meckes DG. 2016. ExtraPEG: a polyethylene glycol-based method for enrichment of extracellular vesicles. Sci. Rep. 6: 23978.
9	Zhao L, Wang H, Fu J, Wu X, Liang XY, Liu XY, et al. 2022. Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis. Biosens. Bioelectron. 214: 114487.
10	Jia Y, Ni Z, Sun H, Wang C. 2019. Microfluidic approaches toward the isolation and detection of exosome nanovesicles. IEEE 7: 45080-45098.
11	Wu M, Ouyang Y, Wang Z, Zhang R, Huang PH, Chen CY, et al. 2017. Isolation of exosomes from whole blood by integrating acoustics and microfluidics. Proc. Natl. Acad. Sci. USA 114: 10584-10589 DOI
12	Yang Q, Cheng L, Hu L, Lou DD, Zhang T, Li JY, et al. 2020. An integrative microfluidic device for isolation and ultrasensitive detection of lung cancer-specific exosomes from patient urine. Biosens. Bioelectron. 163: 112290.
13	Tayebi M, Zhou Y, Tripathi P, Chandramohanadas R, Ai Y. 2020. Exosome purification and analysis using a facile microfluidic hydrodynamic trapping device. Anal. Chem. 92: 10733-10742. DOI
14	Zarovni N, Corrado A, Guazzi P, Zocco D, Lari El, Radano G, et al. 2015. Integrated isolation and quantitative analysis of exosome shuttled proteins and nucleic acids using immunocapture approaches. Methods 87: 46-58. DOI
15	Wang Z, Zong S, Wang Y, Li N, Li L, Lu J, et al. 2018. Screening and multiple detection of cancer exosomes using an SERS-based method. Nanoscale 10: 9053-9062. DOI
16	Gao X, Ran N, Dong X, Zuo BF, Yang R, Zhou QB, et al. 2018. Anchor peptide captures, targets, and loads exosomes of diverse origins for diagnostics and therapy. Sci. Transl. Med. 10: eaat0195.
17	Yang J, Pan B, Zeng F, He BS, Gao YF, Liu XL, et al. 2021. Magnetic colloid antibodies accelerate small extracellular vesicles isolation for point-of-care diagnostics. Nano Lett. 21: 2001-2009. DOI
18	Wang J, Cai X, Wang Z, Chen XQ, Kun L, Cheng H, et al. 2019. Isolation and identification of exosomes from human adipose-derived mesenchymal stem cells. Chinese J. Tissue Eng. Res. 23: 2651.
19	Nakai W, Yoshida T, Diez D, Miyatake YJ, Nishibu T, Imawaka N, et al. 2016. A novel affinity-based method for the isolation of highly purified extracellular vesicles. Sci. Rep. 6: 33935.
20	Lin S, Yu Z, Chen D, Wang ZG, Miao JM, Li QC, et al. 2020. Progress in microfluidics-based exosome separation and detection technologies for diagnostic applications. Small 16: 1903916.
21	Abramowicz A, Widlak P, Pietrowska M. 2016. Proteomic analysis of exosomal cargo: the challenge of high purity vesicle isolation. Mol. Biosyst. 12: 1407-1419. DOI
22	Wang L, Liu L, Liu J. 2021. Advances in isolation and purification techniques for exosomes. Chemistry 84: 1023-1030.
23	Fraser KB, Moehle MS, Daher JPL, Webber PJ, Williams JY, Stewart CA, et al. 2013. LRRK2 secretion in exosomes is regulated by 14-3-3. Hum. Mol. Genet. 22: 4988-5000. DOI
24	Banchereau J, Briere F, Caux C, Banchereau J, Briere F, Caux C, et al. 2000. Immunobiology of dendritic cells. Annu. Rev. Immunol. 18: 767-811. DOI
25	Kim SH, Bianco NR, Shufesky WJ, Morelli AE, Robbins PD. 2007. Effective treatment of inflammatory disease models with exosomes derived from dendritic cells genetically modified to express IL-4. J. Immunol. 179: 2242-2249. DOI
26	Nazimek K, Askenase P W, Bryniarski K. 2018. Antibody light chains dictate the specificity of contact hypersensitivity effector cell suppression mediated by exosomes. Int. J. Mol. Sci. 19: 2656.
27	Liu Z, Lee J, Krummey S, Lu W, Cai HB, Lenardo MJ. 2011. The kinase LRRK2 is a regulator of the transcription factor NFAT that modulates the severity of inflammatory bowel disease. Nat. Immunol. 12: 1063-1070. DOI
28	Qu P, Zhao J, Hu H, Cao WB, Zhang YR, Qi J, et al. 2022. Loss of renewal of extracellular vesicles: harmful effects on embryo development in vitro. Int. J. Nanomed. 17: 2301-2318. DOI
29	Ighodaro OM, Akinloye OA. 2018. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): their fundamental role in the entire antioxidant defence grid. Alex J. Med. 54: 287-293.
30	Wang Y, Branicky R, Noe A, Hekimi S. 2018. Superoxide dismutases: dual roles in controlling ROS damage and regulating ROS signaling. J. Cell Biol. 217: 1915-1928. DOI
31	Ishihara Y, Takemoto T, Itoh K, Yamazaki T. 2015. Dual role of superoxide dismutase 2 induced in activated microglia: oxidative stress tolerance and convergence of inflammatory responses. J. Biol. Chem. 290: 22805-22817. DOI
32	Console L, Scalise M, Indiveri C. 2019. Exosomes in inflammation and role as biomarkers. Clin. Chim. Acta 488: 165-171. DOI
33	Liu FB. 2018. Inflammasome-derived exosomes activate NF-κB signaling in macrophages. Anhui Medical University.
34	McFadden JP, Puangpet P, Basketter DA, Dearman RJ, Kimber I. 2013. Why does allergic contact dermatitis exist?. Br. J. Dermatol. 168: 692-699. DOI
35	Bretz NP, Ridinger J, Rupp AK, Rimbach K, Keller S, Rupp C, et al. 2013. Body fluid exosomes promote secretion of inflammatory cytokines in monocytic cells via Toll-like receptor signaling. J. Biol. Chem. 288: 36691-36702. DOI
36	Guo L, Lai P, Wang Y, Huang T, Chen XM, Luo CW, et al. 2019. Extracellular vesicles from mesenchymal stem cells prevent contact hypersensitivity through the suppression of Tc1 and Th1 cells and expansion of regulatory T cells. Int. Immunopharmacol. 74: 105663.
37	Mohanty A, Tiwari-Pandey R, Pandey NR. 2019. Mitochondria: the indispensable players in innate immunity and guardians of the inflammatory response. J. Cell Commun. Signal. 13: 303-318. DOI
38	Hough KP, Trevor JL, Strenkowski JG, Wang Y, Chacko BK, Tousif S, et al. 2018. Exosomal transfer of mitochondria from airway myeloid-derived regulatory cells to T cells. Redox Biol. 18: 54-64. DOI
39	Shahzad B, Tanveer M, Rehman A, Cheema SA, Fahad S, Rehman S, et al. 2018. Nickel; whether toxic or essential for plants and environment-A review. Plant Physiol. Biochem. 132: 641-651. DOI
40	Xin R, Pan YL, Wang Y, Wang SY, Wang R, Xia B, et al. 2019. Nickel-refining fumes induce NLRP3 activation dependent on mitochondrial damage and ROS production in Beas-2B cells. Arch. Biochem. Biophys. 676: 108148.
41	Uccelli A, Moretta L, Pistoia V. 2008. Mesenchymal stem cells in health and disease. Nat. Rev. Immunol. 8: 726-736. DOI
42	Nazimek K, Bryniarski K, Ptak W, Kormelink TG, Askenase PW. 2020. Orally administered exosomes suppress mouse delayed-type hypersensitivity by delivering miRNA-150 to antigen-primed macrophage APC targeted by exosome-surface anti-peptide antibody light chains. Int. J. Mol. Sci. 21: 5540.
43	Spiewak R. 2008. Patch testing for contact allergy and allergic contact dermatitis. Open Allergy J. 1: 42-51. DOI
44	Vennegaard MT, Bonefeld CM, Hagedorn PH, Bangsgaard N, Lovendorf MB, Odum N, et al. 2012. Allergic contact dermatitis induces upregulation of identical microRNAs in humans and mice. Contact Dermatitis 67: 298-305. DOI
45	Pang Y, Deng C, Geng S, Weng JY, Lai PL, Liao PJ, et al. 2017. Premature exhaustion of mesenchymal stromal cells from myelodysplastic syndrome patients. Am. J. Transl. Res. 9: 3462.
46	Golubinskaya PA, Sarycheva MV, Dolzhikov AA, Bondarev VP, Stefanova MS, Soldatov VO, et al. 2021. Application of multipotent mesenchymal stem cell Secretome in the treatment of adjuvant arthritis and contact-allergic dermatitis in animal models. Pharm. Pharmacol. 8: 416-425. DOI
47	Li Y. 2019. The effects and mechanism of exosomes derived from human umbilical cord mesenchymal stem cells on contact hypersensitivity. South China University of Technology.
48	Mellor AL, Keskin DB, Johnson T, Chandler P, Munn DH. 2002. Cells expressing indoleamine 2, 3-dioxygenase inhibit T cell responses. J. Immunol. 168: 3771-3776. DOI
49	Yan ML, Wang YD, Tian YF, Lai ZD, Yan LN. 2010. Inhibition of allogeneic T-cell response by Kupffer cells expressing indoleamine 2, 3-dioxygenase. World J. Gastroenterol. 16: 636.
50	Kim SH, Lechman ER, Bianco N, Menon R, Keravala A, Nash J, et al. 2005. Exosomes derived from IL-10-treated dendritic cells can suppress inflammation and collagen-induced arthritis. J. Immunol. 174: 6440-6448. DOI
51	Bianco NR, Kim SH, Ruffner MA, Robbins PD. 2009. Therapeutic effect of exosomes from indoleamine 2, 3-dioxygenase-positive dendritic cells in collagen-induced arthritis and delayed-type hypersensitivity disease models. Arthritis Rheum. 60: 380-389. DOI
52	Fernandez-Messina L, Rodriguez-Galan A, de Yebenes VG, Gutierrez-Vazquez C, Tenreiro S, Seabra MC, et al. 2020. Transfer of extracellular vesicle-microRNA controls germinal center reaction and antibody production. EMBO Rep. 21: e48925.
53	Zhang H, Freitas D, Kim HS, Fabijanic K, Li Z, Chen HY, et al. 2018. Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation. Nat. Cell Biol. 20: 332-343. DOI
54	Zabeo D, Cvjetkovic A, Lasser C, Schorb M, Lotvall J, Hoog JL. 2017. Exosomes purified from a single cell type have diverse morphology. J. Extracell. Vesicles 6: 1329476.
55	Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, et al. 2020. Decoy exosomes provide protection against bacterial toxins. Nature 579: 260-264. DOI
56	Sjoqvist S, Kasai Y, Shimura D, Ishikawa T, Ali N, Iwata T, et al. 2019. Oral keratinocyte-derived exosomes regulate proliferation of fibroblasts and epithelial cells. Biochem. Biophys. Res. Commun. 514: 706-718. DOI
57	Li Q, Zhao H, Chen W, Huang P, Bi J. 2019. Human keratinocyte-derived microvesicle miRNA-21 promotes skin wound healing in diabetic rats through facilitating fibroblast function and angiogenesis. Int. J. Biochem. Cell Biol. 114: 105570.
58	Nguyen SH, Dang TP, MacPherson C, Maibach H, Maibach HI. 2008. Prevalence of patch test results from 1970 to 2002 in a multicentre population in North America (NACDG). Contact Dermatitis 58: 101-106.
59	Angioni R, Liboni C, Herkenne S, Sanchez-Rodriguez R, Borile G, Marcuzzi E, et al. 2020. CD73⁺ extracellular vesicles inhibit angiogenesis through adenosine A2B receptor signalling. J. Extracell. Vesicles 9: 1757900.
60	Zhu T, Wang Y, Jin H, Li L. 2019. The role of exosome in autoimmune connective tissue disease. Ann. Med. 51: 101-108.
61	Tan CH, Rasool S, Johnston GA. 2014. Contact dermatitis: allergic and irritant. Clin. Dermatol. 32: 116-124. DOI
62	Nassau S, Fonacier L. 2020. Allergic contact dermatitis. Med. Clin. 104: 61-76.
63	Cordoba S, Garcia-Donoso C, Villanueva CA, Jesus Borbujo. 2011. Allergic contact dermatitis from a veterinary antiinflammatory gel containing 2-hydroxyethyl salicylate. Dermatitis 22: 171-172. DOI
64	Ramos L, Cabral R, Goncalo M. 2014. Allergic contact dermatitis caused by acrylates and methacrylates-a 7-year study. Contact Dermatitis 71: 102-107.
65	Koumaki D, Bergendorff O, Bruze M, Jesus B. 2019. Allergic contact dermatitis to shin pads in a hockey player: acetophenone is an emerging allergen. Dermatitis 30: 162-163. DOI
66	Jacob SE, Matiz C, Herro EM. 2011. Compositae-associated allergic contact dermatitis from bisabolol. Dermatitis 22: 102-105. DOI
67	T Herro EM, Jacob SE. 2012. Butylhydroxytoluene - from jet fuels to cosmetics?. Dermatitis 23: 90-91. DOI
68	Aakhus AE, Warshaw EM. 2011. Allergic contact dermatitis from cetyl alcohol. Dermatitis 22: 56-57. DOI
69	Bruze M, Zimerson E. 2011. Dimethyl fumarate. Dermatitis 22: 3-7. DOI
70	Suzuki K, Hirokawa K, Yagami A, Kayoko M. 2011. Allergic contact dermatitis from carmine in cosmetic blush. Dermatitis 22: 348-349.
71	Schnuch A, Geier J, Uter W, Peter J Frosch. 2007. Majantol^®-a new important fragrance allergen. Contact Dermatitis 57: 48-50. DOI
72	Gonzalez-Perez R, Trebol I, Garcia-Rio I, Arregui MA, Soloieta R. 2007. Allergic contact dermatitis from methylene-bisbenzotriazolyl tetramethylbutylphenol (Tinosorb M (R)). Contact Dermatitis 56: 121.
73	Urwin R, Wilkinson M. 2013. Methylchloroisothiazolinone and methylisothiazolinone contact allergy: a new 'epidemic'. Contact Dermatitis 68: 253-255.
74	Kornik R, Zug KA. 2008. Nickel. Dermatitis 19: 3-8. DOI
75	Mose AP, Frost S, Ohlund U, Andersen KE. 2013. Allergic contact dermatitis from octylisothiazolinone. Contact Dermatitis 69: 49-52. DOI
76	Tran A, Pratt M, DeKoven J. 2010. Acute allergic contact dermatitis of the lips from peppermint oil in a lip balm. Dermatitis 21: 111-115. DOI
77	DeLeo VA. 2006. p-Phenylenediamine. Dermatitis 17: 53-55.
78	Cressey B. 2012. Contact allergy to sorbitans: a follow-up study. Dermatitis 23: 158-161. DOI
79	Jin L, Cao JJ. 2020. Update of immunological mechanism of allergic contact dermatitis. China J. Leprosy Skin Dis. 36: 61-64.
80	Peng XB. 1993. Pathogenesis of allergic and irritant contact dermatitis. Int. J. Dermatol. Venereol. 5: 307-308.
81	Nosbaum A, Vocanson M, Rozieres A, Hennino A, Nicolas JF. 2009. Allergic and irritant contact dermatitis. Eur. J. Dermatol. 19: 325-332. DOI
82	Wei RY, Zhao ZT, Chen TC, Diao Y, Li Z, Gao H, et al. 2016. Immune mechanism of allergic contact dermatitis. Chinese J. Allergy Clin. Immunol. 10: 255-263.
83	Zhao L, Li LF. 2015. The role of regulatory T cells in allergic contact dermatitis. J. Pract. Dermatol. 8: 201-204.
84	DeKoven JG, Silverberg JI, Warshaw EM, Atwater AR, Reeder MJ, Sasseville D, et al. 2021. North American contact dermatitis group patch test results: 2017-2018. Dermatitis 32: 111-123. DOI
85	Chen JK, Jacob SE, Nedorost ST, Hanifin JM, Simpson EL, Boguniewicz M, et al. 2016. A pragmatic approach to patch testing atopic dermatitis patients: clinical recommendations based on expert consensus opinion. Dermatitis 27: 186-278. DOI
86	Owen JL, Vakharia PP, Silverberg JI. 2018. The role and diagnosis of allergic contact dermatitis in patients with atopic dermatitis. Am. J. Clin. Dermatol. 19: 293-302. DOI
87	Lim HW, Collins SAB, Resneck JSJr, Bolognia JL, Hodge JA, Rohrer TA, et al. 2017. The burden of skin disease in the United States. J. Am. ACAD Dermatol. 76: 958-1030. DOI
88	Mowad CM, Anderson B, Scheinman P, Pootongkam S, Nedorost S, Brod B, et al. 2016. Allergic contact dermatitis: patient diagnosis and evaluation. J. Am. ACAD Dermatol. 74: 1029-1069. DOI
89	Pereira N, Coutinho I, Andrade P, Margarida G. 2013. The UV filter Tinosorb M, containing decyl glucoside, is a frequent cause of allergic contact dermatitis. Dermatitis 24: 41-43. DOI
90	European Multicentre Photopatch Test Study (EMCPPTS) Taskforce. 2012. A European multicentre photopatch test study. Br. J. Dermatol. 166: 1002-1009. DOI
91	Van Niel G, D'Angelo G, Raposo G. 2018. Shedding light on the cell biology of extracellular vesicles. Nat. Rev. Mol. Cell Biol. 19: 213-228. DOI
92	Kostner L, Anzengruber F, Guillod C, Recher M, Schmid-Grendelmeier P, Navarini AA. 2017. Allergic contact dermatitis. Immunol. Allergy Clin. 37: 141-152.
93	Batagov AO, Kurochkin IV. 2013. Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3'-untranslated regions. Biol. Direct 8: 12.
94	Igami K, Uchiumi T, Ueda S, Kamioka K, Setoyama D, Gotoh K, et al. 2020. Characterization and function of medium and large extracellular vesicles from plasma and urine by surface antigens and Annexin V. PeerJ. Anal. Chem. 2: e4.
95	Elsharkasy OM, Nordin JZ, Hagey DW, Jong OG, Schiffelers RM, ELAndaloussi S, et al. 2020. Extracellular vesicles as drug delivery systems: why and how?. Adv. Drug Deliv. Rev. 159: 332-343. DOI
96	Bang C, Batkai S, Dangwal S, Gupta SK, Foinquinos A, Holzmann A, et al. 2014. Cardiac fibroblast-derived microRNA passenger strand-enriched exosomes mediate cardiomyocyte hypertrophy. J. Clin. Invest. 124: 2136-2146. DOI
97	Yu Y, Abudula M, Li C, Chen ZB, Zhang Y, Chen YC. 2019. Icotinib-resistant HCC827 cells produce exosomes with mRNA MET oncogenes and mediate the migration and invasion of NSCLC. Respir. Res. 20: 217.
98	He Y, He Z, Leone S, Liu SB. 2021. Milk exosomes transfer oligosaccharides into macro phages to modulate immunity and Attenuate Adherent-Invasive E. coli (AIEC) infection. Nutrients 13: 3198.
99	Guo BB, Bellingham SA, Hill AF. 2016. Stimulating the release of exosomes increases the intercellular transfer of prions. J. Biol. Chem. 291: 5128-5137. DOI
100	Hong Y, Lee J, Vu TH, Lee S, Lillehoj HS, Hong YH. 2021. Exosomes of lipopolysaccharide-stimulated chicken macrophages modulate immune response through the MyD88/NF-κB signaling pathway. Dev. Comp. Immunol. 115: 103908.
101	Alhasan AH, Patel PC, Choi CHJ, Mirkin CA. 2014. Exosome encased spherical nucleic acid gold nanoparticle conjugates as potent microRNA regulation agents. Small 10: 186-192. DOI
102	Li P, Kaslan M, Lee SH, Yao J, Gao ZQ. 2017. Progress in exosome isolation techniques. Theranostics. 7: 789-804. DOI
103	Coughlan C, Bruce K D, Burgy O, Boyd TD, Michel CR, Garcia-Perez JE, et al. 2020. Exosome isolation by ultracentrifugation and precipitation and techniques for downstream analyses. Curr. Protoc. Cell Biol. 88: e110.
104	Gardiner C, Vizio DD, Sahoo S, Thery C, Witwer KW, Wauben M, et al. 2016. Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey. J. Extracell. Vesicles 5: 32945.
105	Thery C, Amigorena S, Raposo G, Clayton A. 2006. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr. Protoc. Cell Biol. 30: 3.22. 1-3.22. 29.
106	Van Deun J, Mestdagh P, Sormunen R, Cocquyt V, Vermaelen K, Vandesompele J, et al. 2014. The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J. Extracell. Vesicles 3: 24858.
107	Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, et al. 2012. Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods 56: 293-304. DOI
108	Gao M, Cai J, Zitkovsky HS, Chen B, Guo LF. 2022. Comparison of yield, purity, and functional properties of large-volume exosome isolation using ultrafiltration and polymer-based precipitation. Methods Mol. Biol. 149: 638-649.
109	Perez-Gonzalez R, Gauthier SA, Kumar A, Saito M, Saito M, Levy E. 2017. A method for isolation of extracellular vesicles and characterization of exosomes from brain extracellular space. Methods Moi. Biol. 2017: 139-151.
110	Street JM, Koritzinsky EH, Glispie DM, Yuen PST. 2017. Urine exosome isolation and characterization. Methods Mol. Biol. 2017: 413-423.
111	Musante L, Tataruch D, Gu D, Benito-Martin A, Calzaferri G, Aherne S, et al. 2014. A simplified method to recover urinary vesicles for clinical applications and sample banking. Sci. Rep. 4: 7532.
112	Muller L, Hong CS, Stolz DB, Watkinsab SC, Whiteside TL. 2014. Isolation of biologically-active exosomes from human plasma. J. Immunol. Methods 411: 55-65. DOI
113	Cheruvanky A, Zhou H, Pisitkun T, Kopp JB, Knepper MA, Yuen PST, et al. 2007. Rapid isolation of urinary exosomal biomarkers using a nanomembrane ultrafiltration concentrator. Am. J. Physiol. Renal Physiol. 292: F1657-F1661. DOI
114	Alvarez ML, Khosroheidari M, Ravi RK, DiStefano1 JK. 2012. Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers. Kidney Int. 82: 1024-1032. DOI
115	Boing AN, Van Der Pol E, Grootemaat AE, Coumans FAW, Sturk A, Nieuwland R. 2014. Single-step isolation of extracellular vesicles by size-exclusion chromatography. J. Extracell. Vesicles 3: 23430.
116	Welton JL, Webber JP, Botos LA, Jones M, Clayton A. 2015. Ready-made chromatography columns for extracellular vesicle isolation from plasma. J. Extracell. Vesicles 4: 27269.