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http://dx.doi.org/10.4014/jmb.2206.06039

Hygrolansamycins A-D, O-Heterocyclic Macrolides from Streptomyces sp. KCB17JA11  

Jang, Jun-Pil (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Lee, Byeongsan (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Heo, Kyung Taek (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Oh, Tae Hoon (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Lee, Hyeok-Won (Biotechnology Process Engineering Center, Korea Research Institute of Bioscience and Biotechnology)
Ko, Sung-Kyun (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Hwang, Bang Yeon (College of Pharmacy, Chungbuk National University)
Jang, Jae-Hyuk (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Hong, Young-Soo (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Microbiology and Biotechnology / v.32, no.10, 2022 , pp. 1299-1306 More about this Journal
Abstract
Six ansamycin derivatives were isolated from the culture broth of Streptomyces sp. KCB17JA11, including four new hygrolansamycins A-D (1-4) and known congeners divergolide O (5) and hygrocin C (6). Compounds 1-5 featured an unusual six-membered O-heterocyclic moiety. The isolation workflow was guided by a Molecular Networking-based dereplication strategy. The structures of 1-4 were elucidated using NMR and HRESIMS experiments, and the absolute configuration was established by the Mosher's method. Compound 2 exhibited mild cytotoxicity against five cancer cell lines with IC50 values ranging from 24.60 ± 3.37 µM to 49.93 ± 4.52 µM.
Keywords
Molecular networking; Streptomyces; ansamycin family; hygrolansamycin;
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