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http://dx.doi.org/10.4014/jmb.2102.02005

Knockdown of Circ_0000144 Suppresses Cell Proliferation, Migration and Invasion in Gastric Cancer Via Sponging MiR-217  

Ji, Fengcun (Department of General Surgery, Sunshine Union Hospital)
Lang, Chao (Department of General Surgery, Sunshine Union Hospital)
Gao, Pengfei (Department of General Surgery, Sunshine Union Hospital)
Sun, Huanle (Department of General Surgery, Sunshine Union Hospital)
Publication Information
Journal of Microbiology and Biotechnology / v.31, no.6, 2021 , pp. 784-793 More about this Journal
Abstract
Previous studies have uncovered the role of circ_0000144 in various tumors. Here, we investigated the function and mechanism of circ_0000144 in gastric cancer (GC) progression. The expression of circ_0000144 in GC tissues and cells was detected through quantitative real-time polymerase chain reaction (qRT-PCR) method. Gain- and loss-of-function experiments including colony formation, wound healing and transwell assays were performed to examine the role of circ_0000144 in GC cells. Furthermore, western blot was conducted to determine the expressions of epithelial mesenchymal transition (EMT)-related proteins. The interaction between circ_0000144 and miR-217 was analyzed by bioinformatic analysis and luciferase reporter assays. The circ_0000144 expression was obviously upregulated in GC tissues and cells. Silencing of circ_0000144 inhibited cell proliferation, migration and invasion of GC cells, but ectopic expression of circ_0000144 showed the opposite results. Moreover, circ_0000144 sponged miR-217, and rescue assays revealed that silencing miR-217 expression reversed the inhibitory effect of circ_0000144 knockdown on the progress of GC. Our findings reveal that circ_0000144 inhibition suppresses GC cell proliferation, migration and invasion via absorbing miR-217, providing a new biomarker and potential therapeutic target for treatment of GC.
Keywords
Gastric cancer; circ_0000144; microRNA-217; proliferation; metastasis;
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