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http://dx.doi.org/10.4014/jmb.2012.12036

Identification and Characterization of a Novel Thermostable GDSL-Type Lipase from Geobacillus thermocatenulatus  

Jo, Eunhye (Department of Microbiology, College of Natural Sciences, Pusan National University)
Kim, Jihye (Department of Microbiology, College of Natural Sciences, Pusan National University)
Lee, Areum (Department of Microbiology, College of Natural Sciences, Pusan National University)
Moon, Keumok (Department of Microbiology, College of Natural Sciences, Pusan National University)
Cha, Jaeho (Department of Microbiology, College of Natural Sciences, Pusan National University)
Publication Information
Journal of Microbiology and Biotechnology / v.31, no.3, 2021 , pp. 483-491 More about this Journal
Abstract
Two putative genes, lip29 and est29, encoding lipolytic enzymes from the thermophilic bacterium Geobacillus thermocatenulatus KCTC 3921 were cloned and overexpressed in Escherichia coli. The recombinant Lip29 and Est29 were purified 67.3-fold to homogeneity with specific activity of 2.27 U/mg and recovery of 5.8% and 14.4-fold with specific activity of 0.92 U/mg and recovery of 1.3%, respectively. The molecular mass of each purified enzyme was estimated to be 29 kDa by SDS-PAGE. The alignment analysis of amino acid sequences revealed that both enzymes belonged to GDSL lipase/esterase family including conserved blocks with SGNH catalytic residues which was mainly identified in plants before. While Est29 showed high specificity toward short-chain fatty acids (C4-C8), Lip29 showed strong lipolytic activity to long-chain fatty acids (C12-C16). The optimal activity of Lip29 toward p-nitrophenyl palmitate as a substrate was observed at 50℃ and pH 9.5, respectively, and its activity was maintained more than 24 h at optimal temperatures, indicating that Lip29 was thermostable. Lip29 exhibited high tolerance against detergents and metal ions. The homology modeling and substrate docking revealed that the long-chain substrates showed the greatest binding affinity toward enzyme. Based on the biochemical and insilico analyses, we present for the first time a GDSL-type lipase in the thermophilic bacteria group.
Keywords
Extremophiles; Geobacillus; GDSL esterase/lipase; thermophiles; enzyme characterization;
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