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http://dx.doi.org/10.4014/jmb.2002.02039

Hepatitis B Virus DNA Polymerase Displays an Anti-Apoptotic Effect by Interacting with Elongation Factor-1 Alpha-2 in Hepatoma Cells  

Niu, Xianli (Department of Biochemistry and Molecular Biology, Zunyi Medical University)
Nong, Shirong (Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral Biology, Jinan University)
Gong, Junyuan (Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral Biology, Jinan University)
Zhang, Xin (Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral Biology, Jinan University)
Tang, Hui (Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral Biology, Jinan University)
Zhou, Tianhong (Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral Biology, Jinan University)
Li, Wei (Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral Biology, Jinan University)
Publication Information
Journal of Microbiology and Biotechnology / v.31, no.1, 2021 , pp. 16-24 More about this Journal
Abstract
Hepatitis B virus (HBV) genome P-encoded protein HBV DNA polymerase (Pol) has long been known as a reverse transcriptase during HBV replication. In this study, we investigated the impact of HBV Pol on host cellular processes, mainly apoptosis, and the underlying mechanisms. We showed a marked reduction in apoptotic rates in the HBV Pol-expressed HepG2 cells compared to controls. Moreover, a series of assays, i.e., yeast two-hybrid, GST pull-down, co-immunoprecipitation, and confocal laser scanning microscopy, identified the host factor eEF1A2 to be associated with HBV Pol. Furthermore, knockdown of eEF1A2 gene by siRNA abrogated the HBV Pol-mediated anti-apoptotic effect with apoptosis induced by endoplasmatic reticulum (ER) stress-inducer thapsigargin (TG), thus suggesting that the host factor eEF1A2 is essential for HBV Pol's anti-apoptosis properties. Our findings have revealed a novel role for HBV Pol in its modulation of apoptosis through integrating with eEF1A2.
Keywords
Hepatitis B virus; chronic hepatitis B; HBV DNA polymerase; apoptosis; eEF1A2;
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