Browse > Article
http://dx.doi.org/10.4014/jmb.2003.03050

Antioxidant and Anti-Inflammatory Effects of NCW Peptide from Clam Worm (Marphysa sanguinea)  

Park, Young Ran (Department of Dental Pharmacology, School of Dentistry, Jeonbuk National University)
Park, Chan-Il (Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University)
Soh, Yunjo (Department of Dental Pharmacology, School of Dentistry, Jeonbuk National University)
Publication Information
Journal of Microbiology and Biotechnology / v.30, no.9, 2020 , pp. 1387-1394 More about this Journal
Abstract
Clam worms (Marphysa sanguinea) are a rich source of bioactive components such as the antibacterial peptide, perinerin. In the present study, we explored the physiological activities of a novel NCWPFQGVPLGFQAPP peptide (NCW peptide), which was purified from clam worm extract through high-performance liquid chromatography. Tandem mass spectrometry (MS/MS) revealed that NCW was a new peptide with a molecular weight of 1757.86 kDa. Moreover, NCW peptide exhibited significant antioxidant effects, causing a 50% inhibition of DPPH radical at a concentration of 20 μM without showing any cytotoxicity. These were associated with a reduction in the activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in LPS-stimulated RAW264. 7 cells. Furthermore, NCW peptide exhibited anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via inhibition of the abnormal production of pro-inflammatory cytokines including nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). These anti-inflammatory effects of NCW peptide were associated with the inhibition of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Our results therefore suggest that this novel NCW peptide with antioxidant and anti-inflammatory effects could be a good therapeutic agent against inflammation-related diseases.
Keywords
Novel peptide; clam worm; antioxidant; anti-inflammation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ovchinnikova TV, Aleshina GM, Balandin SV, Krasnosdembskaya AD, Markelov ML, Frolova EI, et al. 2004. Purification and primary structure of two isoforms of arenicin, a novel antimicrobial peptide from marine polychaeta Arenicola marina. FEBS Lett. 577: 209-214.   DOI
2 Kim SW, Sapkota M, Yang M, Li L, Soh Y. 2017. Anti-inflammatory and antioxidant effects of clam worm extract treated with peptidoglycan. Kor. J. Pharmacogn. 48: 187-194.
3 Kim SW, Sapkota M, Li L, Yang M, Park CI, Soh Y. 2016. Anti-inflammatory and antioxidant effects of clam worm extract in macrophage RAW264.7 cells. Kor. J. Pharmacogn. 47: 150-157.
4 Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 26: 1199-1200.   DOI
5 Aebi H. 1984. Catalase in vitro. Methods Enzymol. 105: 121-126.   DOI
6 Buege AJ, Aus SD. 1978. Microsomal lipid peroxidation. Methods Enzymol. 52: 302-310.   DOI
7 Prasad K, Mantha SV, Muir AD. Westcott ND. 2000. Protective effect of secoisolariciresinol diglucoside against streptozotocin-induced diabetes and its mechanism. Mol. Cell. Biochem. 206: 141-149.   DOI
8 Popovic M, Hrcenjak TM, Babic T. Kos J. Grdisa M. 2001. Effect of earthworm (G-90) extract on formation and lysis of clots originated from venous blood of dogs with cardiopathies and with malignant tumors. Pathol. Oncol. Res. 7: 197-202.   DOI
9 Forrester SJ, Kikuchi DS, Hernandes MS, Xu Q, Griendling KK. 2018. Reactive oxygen species in metabolic and inflammatory signaling. Circ. Res. 16:877-902.
10 Hajjar DP, Gotto AM Jr. 2013. Biological relevance of inflammation and oxidative stress in the pathogenesis of arterial diseases. Am. J. Pathol. 182: 1474-1481.   DOI
11 Vaziri ND, Rodriguez-Iturbe B. 2006. Mechanisms of disease: oxidative stress and inflammation in the pathogenesis of hypertension. Nat. Clin. Pract. Nephrol. 2: 582-593.   DOI
12 Pigeolet E, Corbisier P, Houbion A, Lambert D, Michiels C, Raes M, et al. 1990. Glutathione peroxidase, superoxide dismutase, and catalase inactivation by peroxides and oxygen derived free radicals. Mech. Ageing Dev. 51: 283-297.   DOI
13 Meghana K, Sanjeev G, Ramesh B. 2007. Curcumin prevents streptozotocin-induced islet damage by scavenging free radicals: a prophylactic and protective role. Eur. J. Pharmacol. 577: 183-191.   DOI
14 Kuo MY, Liao MF, Chen FL, Li YC, Yang ML, Lin RH, et al. 2011. Luteolin attenuates the pulmonary inflammatory response involves abilities of antioxidation and inhibition of MAPK and NFkappaB pathways in mice with endotoxin-induced acute lung injury. Food Chem. Toxicol. 49: 2660-2666.   DOI
15 Gao Y, Jiang W, Dong C, Li C, Fu X, Min L, et al. 2012. Anti-inflammatory effects of sophocarpine in LPS-induced RAW 264.7 cells via $NF-{\kappa}B$ and MAPKs signaling pathways. Toxicol. In Vitro 26: 1-6.   DOI
16 Sheeba MS, Asha VV. 2009. Cardiospermum halicacabum ethanol extract inhibits LPS induced COX-2, TNF-alpha and iNOS expression, which is mediated by NF-kappaB regulation, in RAW264.7 cells. J. Ethnopharmacol. 124: 39-44.   DOI
17 Franzotti EM, Santos CV, Rodrigues HM, Mourao RH, Andrade MR, Antoniolli AR. 2000. Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. (Malva-branca). J. Ethnopharmacol. 72: 273-727.   DOI
18 Park JY, Cho HY, Kim JK, Noh KH, Yang JR, Ahn JM, et al. 2005. Chlorella dichloromethane extract ameliorates NO production and iNOS expression through the down-regulation of NF kappa B activity mediated by suppressed oxidative stress in RAW 264.7 macrophages. Clin. Chim. Acta 351:185-196.   DOI
19 Jeyaseelan S, Chu HW, Young SK, Worthen GS. 2004. Transcriptional profiling of lipopolysaccharide-induced acute lung injury. Infect. Immun. 72: 7247-756.   DOI
20 Tasaka S, Amaya F, Hashimoto S, Ishizaka A. 2008. Roles of oxidants and redox signaling in the pathogenesis of acute respiratory distress syndrome. Antioxid. Redox. Signal. 10: 739-754.   DOI
21 Balamurugan M, Parthasarathi K, Ranganathan LS, Cooper EL. 2008. Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties. J. Zhejiang Univ. Sci. B. 9: 141-147.   DOI
22 Zeng KW, Yu Q, Liao LX, Song FJ, Lv HN, Jiang Y, et al. 2015. Anti-neuroinflammatory effect of MC13, a novel coumarin compound from condiment murraya, through inhibiting Lipopolysaccharide-induced TRAF6-TAK1-NF-kappaB, P38/ERK MAPKS and Jak2-Stat1/Stat3 pathways. J. Cell. Biochem. 116: 1286-1299.   DOI
23 Sontakke, AN, Tare RS. 2002. A duality in the roles of reactive oxygen species with respect to bone metabolism. Clin. Chim. Acta 318: 145-148.   DOI
24 Petersen KS, Smith C. 2016. Ageing-associated oxidative stress and inflammation are alleviated by products from grapes. Oxid. Med. Cell. Longev. 2016: 6236309.
25 Balamurugan M, Parthasarathi K, Cooper EL, Ranganathan LS. 2007. Earthworm paste (Lampito mauritii, Kinberg) alters inflammatory, oxidative, haematological and serum biochemical indices of inflamed rat. Eur. Rev. Med. Pharmacol. Sci. 11: 77-90.