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http://dx.doi.org/10.4014/jmb.1911.11042

Smilax guianensis Vitman Extract Prevents LPS-Induced Inflammation by Inhibiting the NF-κB Pathway in RAW 264.7 Cells  

Kim, Ju Gyeong (School of Food Science and Biotechnology, Kyungpook National University)
Kim, Min Jeong (School of Food Science and Biotechnology, Kyungpook National University)
Lee, Ji Su (Division of Bioengineering, Incheon National University)
Sydara, Kongmany (Ministry of Health, Institute of Traditional Medicine)
Lee, Sangwoo (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology)
Byun, Sanguine (Department of Biotechnology, Yonsei University)
Jung, Sung Keun (School of Food Science and Biotechnology, Kyungpook National University)
Publication Information
Journal of Microbiology and Biotechnology / v.30, no.6, 2020 , pp. 822-829 More about this Journal
Abstract
Nutraceutical treatments can reduce inflammation and prevent the development of inflammatory diseases. In this study, the anti-inflammatory effects of Smilax guianensis Vitman extract (SGE) were examined. SGE suppressed lipopolysaccharide (LPS)-mediated nitrite production in RAW 264.7 cells. SGE also prevented the LPS-induced expression of inducible nitric oxide synthase (iNOS) but not cyclooxygenase (COX)-2. Western blot analysis showed that SGE attenuated LPS-induced phosphorylation of IκB kinase (IKK), inhibitor of kappa B (IκB), and p65. Additionally, SGE inhibited LPS-induced IκB degradation in RAW 264.7 cells. Western blot analysis of the cytosolic and nuclear fractions, as well as immunofluorescence assay results, revealed that SGE suppressed LPS-induced p65 nuclear translocation in RAW 264.7 cells. Moreover, SGE reduced LPS-induced interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) mRNA expression and IL-1β and IL-6 protein expression in RAW 264.7 cells. Collectively, these results indicate that SGE suppresses the NF-κB signaling pathway and thereby inhibits the production of NO, IL-1β, and IL-6.
Keywords
Smilax guianensis Vitman extract; nutraceuticals; nitric oxide; nitric oxide synthase; inflammation; NF-${\kappa}B$;
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