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http://dx.doi.org/10.4014/jmb.1908.08045

Croton hirtus L'Hér Extract Prevents Inflammation in RAW264.7 Macrophages Via Inhibition of NF-κB Signaling Pathway  

Kim, Min Jeong (School of Food Science and Biotechnology, Kyungpook National University)
Kim, Ju Gyeong (School of Food Science and Biotechnology, Kyungpook National University)
Sydara, Kong Many (Ministry of Health, Institute of Traditional Medicine)
Lee, Sang Woo (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology)
Jung, Sung Keun (School of Food Science and Biotechnology, Kyungpook National University)
Publication Information
Journal of Microbiology and Biotechnology / v.30, no.4, 2020 , pp. 490-496 More about this Journal
Abstract
Consumption of anti-inflammatory nutraceuticals may help treat or prevent inflammation-related illnesses such as diabetes, cardiovascular disease, and cancer. This study evaluated the effect of Croton hirtus L'Hér extract (CHE) on lipopolysaccharide (LPS)-induced nitric oxide (NO) production and nuclear factor kappa-B (NF-κB) signaling cascades. CHE significantly suppressed LPS-induced NO production and inducible nitric oxide synthase (iNOS) expression in RAW264.7 macrophages, although cyclooxygenase (COX)-2 expression was not affected. CHE also suppressed LPS-induced IκB kinase (IKK), IκB, and p65 phosphorylation in RAW264.7 cells. Western blot and immunofluorescence assays of cytosol and nuclear p65 and the catalytic subunit of NF-κB showed that CHE suppressed LPS-induced p65 translocation from the cytosol to the nucleus. CHE also suppressed LPS-induced Interleukin (IL)-6 and tumor necrosis factor (TNF)-α production in RAW264.7 cells. These results suggest that CHE prevents NO-mediated inflammation by suppressing NF-κB and inflammatory cytokines.
Keywords
Croton hirtus $L^{\prime}H{\acute{e}}r$; inflammation; $NF-{\kappa}B$; nutraceuticals; macrophage; nitric oxide;
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