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http://dx.doi.org/10.4014/jmb.2007.07018

Inhibition of Jurkat T Cell Proliferation by Active Components of Rumex japonicus Roots Via Induced Mitochondrial Damage and Apoptosis Promotion  

Qiu, Yinda (College of Pharmacy, Chonnam National University)
Li, Aoding (College of Pharmacy, Chonnam National University)
Lee, Jina (Biometrology Group, Korea Research Institute of Standards and Science (KRISS))
Lee, Jeong Eun (Metabolic Regulation Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Eun-Woo (Metabolic Regulation Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Cho, Namki (College of Pharmacy, Chonnam National University)
Yoo, Hee Min (Biometrology Group, Korea Research Institute of Standards and Science (KRISS))
Publication Information
Journal of Microbiology and Biotechnology / v.30, no.12, 2020 , pp. 1885-1895 More about this Journal
Abstract
Rumex japonicus Houtt (RJH) is a valuable plant used in traditional medicine to treat several diseases, such as scabies and jaundice. In this study, Jurkat cell growth inhibitory extracts of R. japonicus roots were subjected to bioassay-guided fractionation, resulting in the isolation of three naphthalene derivatives (3-5) along with one anthraquinone (6) and two phenolic compounds (1 and 2). Among these compounds, 2-methoxystypandrone (5) exhibited potent anti-proliferative effects on Jurkat cells. Analysis by flow cytometry confirmed that 2-methoxystypandrone (5) could significantly reduce mitochondrial membrane potential and promote increased levels of mitochondrial reactive oxygen species (ROS), suggesting a strong mitochondrial depolarization effect. Real-time quantitative polymerase chain reaction (qPCR) analysis was also performed, and the results revealed that the accumulation of ROS was caused by reduced mRNA expression levels of heme oxygenase (HO-1), catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). In addition, 2-methoxystypandrone (5) triggered strong apoptosis that was mediated by the arrest of the G0/G1 phase of the cell cycle. Furthermore, 2-methoxystypandrone (5) downregulated p-IκB-α, p-NF-κB p65, Bcl2, and Bcl-xl and upregulated BAX proteins. Taken together, these findings revealed that 2-methoxystypandrone (5) isolated from RJH could potentially serve as an early lead compound for leukemia treatment involving intracellular signaling by increasing mitochondrial ROS and exerting anti-proliferative effects.
Keywords
Rumex japonicus Houtt; apoptosis; reactive oxygen species (ROS); Jurkat cells;
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