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http://dx.doi.org/10.4014/jmb.1802.02010

Controllable Biogenic Synthesis of Intracellular Silver/Silver Chloride Nanoparticles by Meyerozyma guilliermondii KX008616  

Alamri, Saad A.M. (Department of Biology, Faculty of Science, King Khalid University)
Hashem, Mohamed (Department of Biology, Faculty of Science, King Khalid University)
Nafady, Nivien A. (Department of Botany and Microbiology, Faculty of Science, Assiut University)
Sayed, Mahmoud A. (Department of Physics, Faculty of Science, King Khalid University)
Alshehri, Ali M. (Department of Biology, Faculty of Science, King Khalid University)
El-Shaboury, Gamal A. (Department of Biology, Faculty of Science, King Khalid University)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.6, 2018 , pp. 917-930 More about this Journal
Abstract
Intracellular synthesis of silver/silver chloride nanoparticles (Ag/AgCl-NPs) using Meyerozyma guilliermondii KX008616 is reported under aerobic and anaerobic conditions for the first time. The biogenic synthesis of Ag-NP types has been proposed as an easy and cost-effective alternative for various biomedical applications. The interaction of nanoparticles with ethanol production was mentioned. The purified biogenic Ag/AgCl-nanoparticles were characterized by different spectroscopic and microscopic approaches. The purified nanoparticles exhibited a surface plasmon resonance band at 419 and 415 nm, confirming the formation of Ag/AgCl-NPs under aerobic and anaerobic conditions, respectively. The planes of the cubic crystalline phase of the Ag/AgCl-NPs were confirmed by X-ray diffraction. Fourier-transform infrared spectra showed the interactions between the yeast cell constituents and silver ions to form the biogenic Ag/AgCl-NPs. The intracellular Ag/AgCl-NPs synthesized under aerobic condition were homogenous and spherical in shape, with an approximate particle size of 2.5-30nm as denoted by the transmission electron microscopy (TEM). The reaction mixture was optimized by varying reaction parameters, including temperature and pH. Analysis of ultrathin sections of yeast cells by TEM indicated that the biogenic nanoparticles were formed as clusters, known as nanoaggregates, in the cytoplasm or in the inner and outer regions of the cell wall. The study recommends using the biomass of yeast that is used in industrial or fermentation purposes to produce Ag/AgCl-NPs as associated by-products to maximize benefit and to reduce the production cost.
Keywords
Ag/AgCl-NPs; ethanol; fermentation; intracellular synthesis; Meyerozyma guilliermondii; X-ray; transmission electron microscope;
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1 Kowshik M, Ashtaputre S, Kulkani SK, Parknikar KMM. 2003. Extracellular synthesis of silver nanoparticles by a silver-tolerant yeast strain MKY3. Nanotechnology 14: 95-100.   DOI
2 Pimprikar PS. 2009. Influence of biomass and gold salt concentration on nanoparticle synthesis by the tropical marine yeast Yarrowia lipolytica NCIM 3589. Colloids Surf. B Biointerfaces 7: 309-316.
3 Cruz D, Fale PL, Mourato A, Vaz PD, Serralheiro ML, Lino AR. 2010. Preparation and physicochemical characterization of Ag nanoparticles biosynthesized by Lippia citriodora (Lemon Verbena). Colloids Surf. B Biointerfaces 81: 67-73.   DOI
4 Baghizadeh A, Ranjbar S, Gupta VK, Asif M, Pourseyedi S, Karimi MJ, et al. 2015. Green synthesis of silver nanoparticles using seed extract of Calendula officinalis in liquid phase. J. Mol. Liq. 207: 159-163.   DOI
5 Muthukumaran, U, Govindarajan M, Rajeswary M, Hoti SL. 2015. Synthesis and characterization of silver nanoparticles using Gmelina asiatica leaf extract against filariasis, dengue, and malaria vector mosquitoes. Parasitol. Res. 114: 1817-1827.   DOI
6 Garg S, Chandra A. 2012. Biosynthesis and anthelmintic activity of silver nanoparticles using aqueous extract of Saraca indica leaves. Int. J. Ther. Appl. 7: 9-12.
7 Marimuthu S, Rahuman AA, Jayaseelan C, Kirthi AV, Santhoshkumar T, Velayutham K, et al. 2013. Acaricidal activity of synthesized titanium dioxide nanoparticles using Calotropis gigantea against Rhipicephalus microplus and Haemaphysalis bispinosa. Asian Pac. J. Trop. Med. 66: 82-688.
8 Shelar GB, Chavan AM. 2014. Fungus-mediated biosynthesis of silver nanoparticles and its antibacterial activity. Arch. Appl. Sci. Res. 6: 111-114.
9 Patel V, Berthold D, Puranik P, Gantar M. 2015. Screening of cyanobacteria and microalgae for their ability to synthesize silver nanoparticles with antibacterial activity. Biotechnol. Rep. 5: 112-119.   DOI
10 Sathiyanarayanan G, Kiran GS, Selvin J. 2013. Synthesis of silver nanoparticles by polysaccharide bioflocculant produced from marine Bacillus subtilis MSBN17. Colloids Surf. B Biointerfaces 102: 13-20.   DOI
11 Suman TY, Rajasree SRR, Kanchana A, Elizabeth SB. 2013. Biosynthesis, characterization and cytotoxic effect of plant mediated silver nanoparticles using Morinda citrifolia root extract. Colloids Surf. B Biointerfaces 106: 74-78.   DOI
12 Mandal S, Phadtare S, Sastry M. 2005. Interfacing biology with nanoparticles. Curr. Appl. Phys. 5: 118-127.   DOI
13 Roopan SM, Madhumitha G, Rahuman AA, Kamaraj C, Bharathi A, Surendra TV. 2013. Low-cost and eco-friendly phyto-synthesis of silver nanoparticles using Coos nucifera Coir extract and its larvicidal activity. Ind. Crops Prod. 43: 631-635.   DOI
14 Singh R, Shedbalkar UU, Wadhwani SA, Chopade BA. 2015. Bacteriagenic silver nanoparticles: synthesis, mechanism, and applications. Appl. Microbiol. Biotechnol. 99: 4579-4593.   DOI
15 Mekkawy AI, El-Mokhtar MA, Nafady NA, Yousef N, Hamad MA, El-Shanawany SM, et al. 2017. In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels. Int. J. Nanomed. 12: 759-777.   DOI
16 Duran N, Marcato PD, Alves OL, De Souza GIH, Esposito E. 2005. Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J. Nanobiotechnology 3: 1-8.   DOI
17 Saifuddin N, Wong CW, Nur Yasumira AA. 2009. Rapid biosynthesis of silver nanoparticles using culture supernatant of bacteria with microwave irradiation. E-J. Chem. 6: 61-70.   DOI
18 Wang P, Huang B, Lou Z, Zhang X, Qin X, Dai Y, et al. 2010. Synthesis of highly efficient Ag/AgCl plasmonic photocatalysts with various structures. Chem. Eur. J. 16: 538-544.   DOI
19 Lee H, Purdon AM, Chu V, Westervelt RM. 2004. Controlled assembly of magnetic nanoparticles from magnetotactic bacteria using microelectromagnets arrays. Nano Lett. 4: 995-998.   DOI
20 Lengke MF, Fleet ME, Southam G. 2007. Biosynthesis of silver nanoparticles by filamentous cyanobacteria from a silver(I) nitrate complex. Langmuir 23: 2694-2699.   DOI
21 Apte M, Girme G, Bankar A, Ravi KA, Zinjarde S. 2013. 3,4-dihydroxy-L-phenylalanine-derived melanin from Yarrowia lipolytica mediates the synthesis of silver and gold nanostructures. J. Nanobiotechnology 11: 1-9   DOI
22 Centeno SA, Shamir J. 2008. Surface enhanced Raman scattering (SERS) and FTIR characterization of the sepia melanin pigment used in works of art. J. Mol. Struct. 873: 149-159.   DOI
23 Nnemeka I, Godwin E-U, Olakunle F, Olushola O, Moses O, Chidozie OP, et al. 2016. Microwave enhanced synthesis of silver nanoparticles using orange peel extracts from Nigeria. Chem. Biomol. Eng. 1: 5-11.
24 Tarangini K, Mishra S. 2013. Production, characterization and analysis of melanin from isolated marine Pseudomonas sp. using vegetable waste. Res. J. Engin. Sci. 2: 40-46.
25 Niraimathi KL, Sudha V, Lavanya R, Brindha P. 2013. Biosynthesis of silver nanoparticles using Alternanthera sessilis (Linn.) extract and their antimicrobial, antioxidant activities. Colloids Surf. B Biointerfaces 102: 288-291.   DOI
26 Prakash P, Gnanaprakasam P, Emmanuel R, Arokiyaraj S, Saravanan M. 2013. Green synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn. for enhanced antibacterial activity against multi drug resistant clinical isolates. Colloids Surf. B Biointerfaces 108: 255-259.   DOI
27 Netala VR, Kotakadi VS, Bobbu P, Gaddam SA, Tartte V. 2016. Endophytic fungal isolate mediated biosynthesis of silver nanoparticles and their free radical scavenging activity and anti-microbial studies. 3 Biotech 6: 1-9.
28 Nanda A, Majeed S. 2014. Enhanced antibacterial efficacy of biosynthesized AgNPs from Penicillium glabrum (MTCC1985) pooled with different drugs. Int. J. Pharm. Tech. Res. 6: 217-223.
29 Dasgupta N, Ranjan S, Rajendran B, Manickam V, Ramalingam C, Avadhani GS, et al. 2016. Thermal coreduction approach to vary size of silver nanoparticle: its microbial and cellular toxicology. Environ. Sci. Pollut. Res. 23: 4149-4163.   DOI
30 Naseem T, Farrukh MA. 2015. Antibacterial activity of green synthesis of iron nanoparticles using Lawsonia inermis and Gardenia jasminoides leaves extract. J. Chem. 2015: 912342.
31 Gaikwad S, Ingle A, Gade A, Rai M, Falanga A, Incoronato N, et al. 2013. Antiviral activity of mycosynthesized silver nanoparticles against herpes simplex virus and human parainfluenza virus type 3. Int. J. Nanomed. 8: 4303-4314.
32 Daenen LG, Houthuijzen JM, Cirkel GA, Roodhart JML, Shaked Y, Voest EE. 2014. Treatment induced host-mediated mechanisms reducing the efficacy of antitumor therapies. Oncogene 33: 1341-1347.   DOI
33 Kou J, Varma RS. 2012. Beet juice-induced green fabrication of plasmonic AgCl/Ag nanoparticles. ChemSusChem. 5: 2435-2441.   DOI
34 Shameli K, Ahmad MB, Zamanian A, Sangpour P, Shabanzadeh P, Abdollahi Y, et al. 2012. Green biosynthesis of silver nanoparticles using Curcuma longa tuber powder. Int. J. Nanomed. 7: 5603-5610.
35 Husseiny SM, Salah TA, Anter HA. 2015. Biosynthesis of size controlled silver nanoparticles by Fusarium oxysporum, their antibacterial and antitumor activities. Beni-Suef Univ. J. Basic Appl. Sci. 4: 225-231.   DOI
36 Mahendra R, Nelson D. 2011. Metal Nanoparticles in Microbiology. Springer, Heidelberg, Germany.
37 Kora AJ, Beedu SR, Jayaraman A. 2012. Size-controlled green synthesis of silver nanoparticles mediated by gum ghatti (Anogeissus latifolia) and its biological activity. Org. Med. Chem. Lett. 2: 17-27.   DOI
38 Gole A, Dash C, Ramakrishnan V, Sainkar SR, Mandale AB, Rao M, et al. 2001. Pepsin-gold colloid conjugates: preparation, characterization, and enzymatic activity. Langmuir 17: 1674-1679.   DOI
39 Bhainsa KC, D'Souza SF. 2006. Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus. Colloids Surf. B Biointerfaces 47: 160-164.   DOI
40 Virkutyte J, Varma RS. 2011. Green synthesis of metal nanoparticles: biodegradable polymers and enzymes in stabilization and surface functionalization. Chem. Sci. 2: 837-846.   DOI
41 Azizi S, Namvar F, Mahdavi M, Ahmad MB, Mohamad R. 2013. Biosynthesis of silver nanoparticles using brown marine macroalga, Sargassum muticum aqueous extract. Materials (Basel) 6: 5942–5950.   DOI
42 Abdel-Hafez SII, Nafady NA, Abdel-Rahim IR, Shaltout AM, Daros J-A, Mohamed AM. 2016. Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani. 3 Biotech 6: 199-211.
43 Abdel-Hafez SII, Nafady NA, Abdel-Rahim IR, Shaltout AM, Mohamed AM. 2016. Biogenesis and optimisation of silver nanoparticles by the endophytic fungus Cladosporium sphaerospermum. Int. J. Nano Chem. 2: 11-19.   DOI
44 Krumov N, Perner-Nochta I, Oder S, Gotcheva V, Angelov A, Posten C. 2009. Production of inorganic nanoparticles by microorganisms. Chem. Eng. Technol. 32: 1026-1035.   DOI
45 Birla SS, Gaikwad SC, Gade AK, Rai MK. 2013. Rapid synthesis of silver nanoparticles from Fusarium oxysporum by optimizing physicocultural conditions. Scientific World Journal 2013: 796018.
46 Duran N, Cuevas R, Cordi L, Rubilar O, Diez MC. 2014. Biogenic silver nanoparticles associated with silver chloride nanoparticles (Ag@AgCl) produced by laccase from Trametes versicolor. Springerplus 3: 645.   DOI
47 Reese RN, Winge DR. 1988. Sulfide stabilization of the cadmiumgamma- glutamyl peptide complex of Schizosaccharomyces pombe. J. Biol. Chem. 263: 12832-12835.
48 Dameron CT, Reese RN, Mehra RK, Kortan AR, Carroll PJ, Steigerwald ML, et al. 1989. Biosynthesis of cadmium sulphide quantum semiconductor crystallites. Nature 338: 596-597.   DOI
49 Eugenio M, Muller N, Frases S, Almeida-Paes R, Lima LMT, Lemgruber L, et al. 2016. Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria. RSC Adv. 6: 9893-9904.   DOI
50 Kurtzman CP, Fell JW. 1998. Definition, classification and nomenclature of the yeasts, pp 3-5. In Kurtzman CP, Fell JW (eds.), The Yeasts, A Taxonomic Study, 4th Ed. Elsevier Science BV, Amsterdam, The Netherlands.
51 Hesham A, Wang Z, Zhang Y, Zhang J, Lv W, Yang M. 2006. Isolation and identification of a yeast strain capable of degrading four and five ring aromatic hydrocarbons. Ann. Microbiol. 56: 109-112.   DOI
52 Kurtzman CP, Robnett CJ. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73: 331-371.   DOI
53 Vigneshwaran N, Kathe AA, Varadarajan PV, Nachane RP, Balasubramanya RH. 2007. Functional finishing of cotton fabrics using silver nanoparticles. J. Nanosci. Nanotechnol. 7: 1893-1897.   DOI
54 Hashem M, Hesham AE-L, Alrumman SA, Alamri SA, Moustafa MFM. 2014. Indigenous yeasts of the rotten date fruits and their potentiality in bioethanol and single-cell protein production. Int. J. Agric. Biol. 16: 752-758.
55 Moghaddam AB, Namvar F, Moniri M, Tahir PM, Azizi S, Mohamad R. 2015. Nanoparticles biosynthesized by fungi and yeast: a review of their preparation, properties, and medical applications. Molecules 20: 16540-16565.   DOI
56 Jha AK, Prasad K, Kulkarni AR. 2008. Yeast mediated synthesis of silver nanoparticles. Int. J. Nanosci. Nanotechnol. 4: 17-22.
57 Duran N, Marcato PD, Alves OL, Souza GI, Esposito E. 2005. Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J. Nanobiotechnology 3: 8.   DOI
58 Ahmad A, Senapati S, Khan MI, Kumar R, Ramani R, Srinivas V, et al. 2003. Intracellular synthesis of gold nanoparticles by a novel alkalotolerant actinomycete, Rhodococcus species. Nanotechnology 14: 824.   DOI
59 Ingle A, Gade A, Pierrat S, Sonnichsen C, Rai M. 2008. Mycosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria. Curr. Nanosci. 4: 141-144.   DOI
60 Birla SS, Tiwari VV, Gade AK, Ingle AP, Yadav AP, Rai, MK. 2009. Fabrication of silver nanoparticles by Phoma glomerata and its combined effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Lett. Appl. Microbiol. 48: 173-179.   DOI
61 Agnihotri M, Joshi S, Kumar AR, Zinjarde S, Kulkarni S. 2009. Biosynthesis of gold nanoparticles by the tropical marine yeast Yarrowia lipolytica NCIM 3589. Mater. Lett. 63: 1231-1234.   DOI
62 Apte M, Sambre D, Gaikawad S, Joshi S, Bankar A, Kumar AR, et al. 2013. Psychrotrophic yeast Yarrowia lipolytica NCYC 789 mediates the synthesis of antimicrobial silver nanoparticles via cell-associated melanin. AMB Express 3: 32.   DOI
63 Said DE, Elsamad LM, Gohar YM. 2012. Validity of silver, chitosan, and curcumin nanoparticles as anti-Giardia agents. Parasitol. Res. 111: 545-554.   DOI
64 Mourato A, Gadanho M, Lino AR, Tenreiro R. 2011. Biosynthesis of crystalline silver and gold nanoparticles by extremophilic yeasts. Bioinorg. Chem. Appl. 2011: 546074.
65 Fernandez JG, Fernandez-Baldo MA, Berni E, Camí G, Duran N, Raba J, et al. 2016. Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi. Process Biochem. 51: 1306-1313.   DOI
66 Kim HS, Lee CR, Im JH, Lee KB, Moehl T, Marchioro A, et al. 2012. Lead iodide perovskite sensitized all-solid-state submicron thin film mesoscopic solar cell with efficiency exceeding 9%. Sci. Rep. 2: 519.   DOI