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http://dx.doi.org/10.4014/jmb.1711.11003

Effects of Myxococcus fulvus KYC4048 Metabolites on Breast Cancer Cell Death  

Lee, Chayul (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Park, Sanghyun (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Ayush, Ikhbayar (Department of Biomedical Science, Graduate School, Kyung Hee University)
Cho, Kyungyun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Kim, Sung Soo (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Kang, Insug (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Choe, Wonchae (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Kim, Yoon-Seong (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Yoon, Kyung-Sik (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.5, 2018 , pp. 765-775 More about this Journal
Abstract
Using MCF7 breast cancer cells, we tested the anticancer activity of metabolites from 130 strains of myxobacteria newly isolated in South Korea. Of these, three strains whose metabolites had high anticancer activity and low cell toxicity were selected and identified by their fruiting body morphology, cell morphology, and 16S rRNA sequence. Strains KYC4030 and KYC4048 were determined to be Myxococcus fulvus, whereas strain KYC4081 was identified as Corallococcus coralloides. We found that metabolites of M. fulvus KYC4048 demonstrated no toxicity in normal cells but specifically induced cancer cell death by suppressing the expression of WNT2B. This discovery highlights the value of assessing the metabolic and biomedical potential of myxobacteria, even those that are already known but were isolated from new areas, and the possible use of metabolites from M. fulvus KYC4048 in cancer treatment.
Keywords
Myxobacteria; WNT2B; anticancer; breast cancer;
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1 Dawid W. 2000. Biology and global distribution of myxobacteria in soils. FEMS Microbiol. Rev. 24: 403-427.   DOI
2 Reichenbach H. 2005. Order VIII. Myxococcales, pp. 1059-1144. In Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds.), Bergey's Manual of Systematic Bacteriology, 2nd Ed. Bergey's Manual Trust, East Lansing, MI.
3 Gerth K, Pradella S, Perlova O, Beyer S, Muller R. 2003. Myxobacteria: proficient producers of novel natural products with various biological activities - past and future biotechnological aspects with the focus on the genus Sorangium. J. Biotechnol. 106: 233-253.   DOI
4 Kim YS, Bae WC, Back SJ. 2003. Bioactive substances from myxobacteria. Korean J. Microbiol. Biotechnol. 31: 1-12.
5 Weissman KJ, Muller R. 2009. A brief tour of myxobacterial secondary metabolism. Bioorg. Med. Chem. 17: 2121-2136.   DOI
6 Gerth K, Bedorf N, Hofle G, Irschik H, Reichenbach H. 1996. Epothilons A and B: antifungal and cytotoxic compounds from Sorangium cellulosum (Myxobacteria). Production, physico-chemical and biological properties. J. Antibiot. (Tokyo) 49: 560-563.   DOI
7 Bollag DM, McQueney PA, Zhu J, Hensens O, Koupal L, Liesch J, et al. 1995. Epothilones, a new class of microtubule- stabilizing agents with a taxol-like mechanism of action. Cancer Res. 55: 2325-2333.
8 Hyun H, Chung J, Kim J, Lee JS, Kwon BM, Son KH, et al. 2008. Isolation of Sorangium cellulosum carrying epothilone gene clusters. J. Microbiol. Biotechnol. 18: 1416-1422.
9 Stein A. 2010. Ixabepilone: providing new hope for women with breast cancer after anthracycline and taxane failure. Clin. J. Oncol. Nurs. 14: 65-71.   DOI
10 Lee FY, Borzilleri R, Fairchild CR, Kim SH, Long BH, Reventos-Suarez, et al. 2001. BMS-247550: a novel epothilone analog with a mode of action similar to paclitaxel but possessing superior antitumor efficacy. Clin. Cancer Res. 7: 1429-1437.
11 Fumoleau P, Coudert B, Isambert N, Ferrant E. 2007. Novel tubulin-targeting agents: anticancer activity and pharmacologic profile of epothilones and related analogues. Ann. Oncol. 18: v9-v15.   DOI
12 Schneiker S, Perlova O, Kaiser O, Gerth K, Alici A, Altmeyer MO, et al. 2007. Complete genome sequence of the myxobacterium Sorangium cellulosum. Nat. Biotechnol. 25: 1281-1289.   DOI
13 Wenzel SC, Muller R. 2009. The impact of genomics on the exploition of the myxobacterial secondary metabolome. Nat. Prod. Rep. 26: 1385-1407.   DOI
14 Ronning CM, Nierman WC. 2008. The genomes of Myxococcus xanthus and Stigmatella aurantiaca, pp. 285-298. In Whitworth DE (ed.), Myxobacteria: Multicellularity and Differentiation. ASM Press, Washington, DC.
15 Reichenbach H, Dworkin M. 1992. The Myxobacteria, pp. 3416-3487. In Balows A, Truper HG, Dworkin M, Harder W, Schleifer KH (eds.). The Prokaryotes, 2nd Ed. Springer Verlag, New York.
16 Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680.   DOI
17 Shin H, Youn J, An D, Cho K. 2013. Production of antimicrobial substances by strains of myxobacteria Corallococcus and Myxococcus. Korean J. Microbiol. Biotechnol. 41: 44-51.   DOI
18 Lee C, Hyun H, Kim D, Cho K. 2009. Isolation of Chondromyces crocatus in pure culture. Korean J. Microbiol. Biotechnol. 37: 316-321.
19 Rainey FA, Ward-Rainey N, Kroppenstedt RM, Stackerbrandt E. 1996. The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int. J. Syst. Bacteriol. 46: 1088-1092.   DOI
20 Spröer C, Reichenbach H, Stackebrandt E. 1999. The correlation between morphological and phylogenetic classification of myxobacteria. Int. J. Syst. Bacteriol. 3: 1255-1262.
21 Clevers H, Nusse R. 2012. Wnt/${\beta}$-catenin signaling and disease. Cell 149: 1192-1205.   DOI
22 Gerth K, Perlova O, Muller R. 2008. Sorangium cellulosum, pp. 329-348. In Whitworth DE (ed.), Myxobacteria: Multicellularity and Differentiation. ASM Press, Washington, D.C.