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http://dx.doi.org/10.4014/jmb.1711.11065

Comparative Analysis of Gut Microbial Communities in Children under 5 Years Old with Diarrhea  

Wen, Hongyu (School of Life Science, Jiangsu Normal University)
Yin, Xin (School of Life Science, Jiangsu Normal University)
Yuan, Zhenya (School of Life Science, Jiangsu Normal University)
Wang, Xiuying (School of Life Science, Jiangsu Normal University)
Su, Siting (School of Life Science, Jiangsu Normal University)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.4, 2018 , pp. 652-662 More about this Journal
Abstract
Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples had been taken from healthy children without diarrhea and marked as the healthy control group; eight samples had been sampled from children with diarrhea caused by dyspepsia and defined as the non-infectious group; and ten samples had been taken from children with diarrhea induced by intestinal infections and identified as the infectious group. We detected the microbial communities of samples by using high-throughput sequencing of 16S rRNA genes. The proportion of aerobic and facultative anaerobic microbes in samples of the infectious group was much higher than in the non-infectious group. In addition, the relative abundance of Enterococcus in the healthy control group was significantly higher than in the non-infectious group and infectious group. This can be used as a potential diagnostic biomarker for diarrhea.
Keywords
Children diarrhea; gut microbial communities; high-throughput sequencing; 16S rRNA genes;
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