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http://dx.doi.org/10.4014/jmb.1712.12016

Protection of Radiation-Induced DNA Damage by Functional Cosmeceutical Poly-Gamma-Glutamate  

Oh, Yu-Jin (Department of Bio and Fermentation Convergence Technology, BK21 PLUS Project, Kookmin University)
Kwak, Mi-Sun (Department of Bio and Fermentation Convergence Technology, BK21 PLUS Project, Kookmin University)
Sung, Moon-Hee (Department of Bio and Fermentation Convergence Technology, BK21 PLUS Project, Kookmin University)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.4, 2018 , pp. 527-533 More about this Journal
Abstract
This study compared the radioprotective effects of high-molecular-weight poly-gamma-glutamate (${\gamma}-PGA$, average molecular mass 3,000 kDa) and a reduced form of glutathione (GSH, a known radioprotector) on calf thymus DNA damage. The radiation-induced DNA damage was measured on the basis of the decreased fluorescence intensity after binding the DNA with ethidium bromide. All the experiments used $^{60}Co$ gamma radiation at 1,252 Gy, representing 50% DNA damage. When increasing the concentration of ${\gamma}-PGA$ from 0.33 to $1.65{\mu}M$, the DNA protection from radiation-induced damage also increased, with a maximum of 87% protection. Meanwhile, the maximal DNA protection when increasing the concentration of GSH was only 70%. Therefore, ${\gamma}-PGA$ exhibited significant radioprotective effects against gamma irradiation.
Keywords
Poly-gamma-glutamate; radioprotective material; gamma radiation; DNA damage; DNA protection;
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1 Skov KA. 1984. The contribution of hydroxyl radical to radiosensitization: a study of DNA damage. Radiat. Res. 99: 502-510.   DOI
2 Roots R, Chatterjee A, Chang P, Lommel L, Blakely E. 1985. Characterization of hydroxyl radical-induced damage after sparsely and densely ionizing irradiation. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 47: 157-166.   DOI
3 Bucher N, Britten C. 2008. G2 checkpoint abrogation and checkpoint kinase-1 targeting in the treatment of cancer. Br. J. Cancer 98: 523-528.   DOI
4 Santivasi WL, Xia F. 2014. Ionizing radiation-induced DNA damage, response, and repair. Antioxid. Redox Signal. 21: 251-259.   DOI
5 Andersen MH, Becker JC, thor Straten P. 2005. Regulators of apoptosis: suitable targets for immune therapy of cancer. Nat. Rev. Drug Discov. 4: 399-409.   DOI
6 Koukourakis M. 2012. Radiation damage and radioprotectants: new concepts in the era of molecular medicine. Br. J. Radiol. 85: 313-330.   DOI
7 Chatterjee A. 2013. Reduced glutathione: a radioprotector or a modulator of DNA-repair activity? Nutrients 5: 525-542.   DOI
8 Selim M, Saha A, Mukherjea KK. 2017. Protection of radiation induced DNA damage by a newly developed molybdenum complex. J. Radioanal. Nucl. Chem. 311: 189-193.   DOI
9 Peterson D, Bensadoun R-J, Roila F, Group EGW. 2011. Management of oral and gastrointestinal mucositis: ESMO clinical practice guidelines. Ann. Oncol. 22: 78-84.
10 Keefe DM, Schubert MM, Elting LS, Sonis ST, Epstein JB, Raber-Durlacher JE, et al. 2007. Updated clinical practice guidelines for the prevention and treatment of mucositis. Cancer 109: 820-831.   DOI
11 Poo H, Park C, Kwak MS, Choi DY, Hong SP, Lee IH, et al. 2010. New biological functions and applications of high-molecular-mass poly-$\gamma$-glutamic acid. Chem. Biodivers. 7: 1555-1562.   DOI
12 Saenger EL. 1980. Manual on early medical treatment of possible radiation injury. Med. Phys. 7: 82-82.   DOI
13 Ramalho AT, Nascimento A. 1991. The fate of chromosomal aberrations in 137Cs-exposed individuals in the Goiania radiation accident. Health Phys. 60: 67-70.   DOI
14 Rosen EM, Day R, Singh VK. 2015. New approaches to radiation protection. Front. Oncol. 4: 381.
15 Maier P, Wenz F, Herskind C. 2014. Radioprotection of normal tissue cells. Strahlenther. Onkol. 190: 745-752.   DOI
16 Johnke RM, Sattler JA, Allison RR. 2014. Radioprotective agents for radiation therapy: future trends. Future Oncol. 10: 2345-2357.   DOI
17 Breen AP, Murphy JA. 1995. Reactions of oxyl radicals with DNA. Free Radic. Biol. Med. 18: 1033-1077.   DOI
18 Slupphaug G, Kavli B, Krokan HE. 2003. The interacting pathways for prevention and repair of oxidative DNA damage. Mutat. Res. 531: 231-251.   DOI
19 Le Caer S. 2011. Water radiolysis: influence of oxide surfaces on $H_2$ production under ionizing radiation. Water 3: 235-253.   DOI
20 Paul SS, Selim M, Saha A, Mukherjea KK. 2014. Synthesis and structural characterization of dioxomolybdenum and dioxotungsten hydroxamato complexes and their function in the protection of radiation induced DNA damage. Dalton Trans. 43: 2835-2848.   DOI
21 Vos O. 1992. Role of endogenous thiols in protection. Adv. Space Res. 12: 201-207.
22 Prasad S, Srivastava S, Singh M, Shukla Y. 2009. Clastogenic effects of glyphosate in bone marrow cells of Swiss albino mice. J. Toxicol. 2009: 308985.
23 Sung MH, Park C, Kim CJ, Poo H, Soda K, Ashiuchi M. 2005. Natural and edible biopolymer poly-$\gamma$-glutamic acid: synthesis, production, and applications. Chem. Record 5: 352-366.   DOI
24 Buescher JM, Margaritis A. 2007. Microbial biosynthesis of polyglutamic acid biopolymer and applications in the biopharmaceutical, biomedical and food industries. Crit. Rev. Biotechnol. 27: 1-19.   DOI
25 Capitani D, De Angelis A, Crescenzi V, Masci G, Segre A. 2001. NMR study of a novel chitosan-based hydrogel. Carbohydr. Polym. 45: 245-252.   DOI
26 Lee T-Y, Kim Y-H, Yoon S-W, Choi J-C, Yang J-M, Kim C-J, et al. 2009. Oral administration of poly-gamma-glutamate induces TLR4- and dendritic cell-dependent antitumor effect. Cancer Immunol. Immunother. 58: 1781.   DOI
27 Shih L, Van Y-T. 2001. The production of poly-($\gamma$-glutamic acid) from microorganisms and its various applications. Bioresour. Technol. 79: 207-225.   DOI
28 Choi J-C, Uyama H, Lee C-H, Sung M-H. 2015. In vivo hair growth promotion effects of ultra-high molecular weight poly-$\gamma$-glutamic acid from Bacillus subtilis (Chungkookjang). J. Microbiol. Biotechnol. 25: 407-412.   DOI
29 Lee T-Y, Kim D-J, Won J-N, Lee I-H, Sung M-H, Poo H. 2014. Oral administration of poly-$\gamma$-glutamate ameliorates atopic dermatitis in Nc/Nga mice by suppressing Th2-biased immune response and production of IL-17A. J. Invest. Dermatol. 134: 704-711.   DOI
30 Kim TW, Lee TY, Bae HC, Hahm JH, Kim YH, Park C, et al. 2007. Oral administration of high molecular mass poly-$\gamma$-glutamate induces NK cell-mediated antitumor immunity. J. Immunol. 179: 775-780.   DOI
31 Park C, Sung M-H. 2009. New bioindustrial development of high molecular weight of poly-gamma-glutamic acid produced by Bacillus subtilis (chungkookjang). Polym. Sci. Technol. 20: 440-446.
32 Suh D. 1999. Cooperative binding interaction of ethidium with allosteric DNA. Exp. Mol. Med. 31: 151-158.   DOI
33 Reichmann M, Rice S, Thomas C, Doty P. 1954. A further examination of the molecular weight and size of desoxypentose nucleic acid. J. Am. Chem. Soc. 76: 3047-3053.   DOI
34 Cai L, Cherian MG. 2003. Zinc-metallothionein protects from DNA damage induced by radiation better than glutathione and copper- or cadmium-metallothioneins. Toxicol. Lett. 136: 193-198.   DOI