Biochemical Characterization of a Novel GH86 β-Agarase Producing Neoagarohexaose from Gayadomonas joobiniege G7 |
Lee, Yeong Rim
(Department of Bioscience and Bioinformatics, Myongji University)
Jung, Subin (Department of Bioscience and Bioinformatics, Myongji University) Chi, Won-Jae (Biological and Genetic Resource Assessment Division, National Institute of Biological Resource) Bae, Chang-Hwan (Biological and Genetic Resource Assessment Division, National Institute of Biological Resource) Jeong, Byeong-Chul (Department of Bioscience and Bioinformatics, Myongji University) Hong, Soon-Kwang (Department of Bioscience and Bioinformatics, Myongji University) Lee, Chang-Ro (Department of Bioscience and Bioinformatics, Myongji University) |
1 |
Jung S, Jeong BC, Hong SK, Lee CR. 2017. Cloning, expression, and biochemical characterization of a novel acidic GH16 |
2 | Ariga O, Inoue T, Kubo H, Minami K, Nakamura M, Iwai M, et al. 2012. Cloning of agarase gene from non-marine agarolytic bacterium Cellvibrio sp. J. Microbiol. Biotechnol. 22: 1237-1244. DOI |
3 |
Ohta Y, Hatada Y, Nogi Y, Li Z, Ito S, Horikoshi K. 2004. Cloning, expression, and characterization of a glycoside hydrolase family 86 |
4 | Chi WJ, Park JS, Kwak MJ, Kim JF, Chang YK, Hong SK. 2013. Isolation and characterization of a novel agar-degrading marine bacterium, Gayadomonas joobiniege gen, nov, sp. nov., from the Southern Sea, Korea. J. Microbiol. Biotechnol. 23: 1509-1518. |
5 | Yan S, Yu M, Wang Y, Shen C, Zhang XH. 2011. Catenovulum agarivorans gen. nov., sp. nov., a peritrichously flagellated, chain-forming, agar-hydrolysing gammaproteobacterium from seawater. Int. J. Syst. Evol. Microbiol. 61: 2866-2873. DOI |
6 | Zor T, Selinger Z. 1996. Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. Anal. Biochem. 236: 302-308. DOI |
7 | Segel IH. 1976. Enzyme kinetics, pp. 214-229. In: Biochemical Calculations. How to Solve Mathematical Problems in General Biochemistry, 2nd Ed. Wiley, New York. |
8 | Shan D, Li X, Gu Z, Wei G, Gao Z, Shao Z. 2014. Draft genome sequence of the agar-degrading bacterium Catenovulum sp. strain DS-2, isolated from intestines of Haliotis diversicolor. Genome Announc. 2: e00144-00114. |
9 | Frey PA. 1996. The Leloir pathway: a mechanistic imperative for three enzymes to change the stereochemical configuration of a single carbon in galactose. FASEB J. 10: 461-470. DOI |
10 | Yun EJ, Lee S, Kim HT, Pelton JG, Kim S, Ko HJ, et al. 2015. The novel catabolic pathway of 3,6-anhydro-L-galactose, the main component of red macroalgae, in a marine bacterium. Environ. Microbiol. 17: 1677-1688. DOI |
11 | Fu XT, Kim SM. 2010. Agarase: review of major sources, categories, purification method, enzyme characteristics and applications. Mar. Drugs 8: 200-218. DOI |
12 | Kobayashi R, Takisada M, Suzuki T, Kirimura K, Usami S. 1997. Neoagarobiose as a novel moisturizer with whitening effect. Biosci. Biotechnol. Biochem. 61: 162-163. DOI |
13 | Hong SJ, Lee JH, Kim EJ, Yang HJ, Park JS, Hong SK. 2017. Anti-obesity and anti-diabetic effect of neoagarooligosaccharides on high-fat diet-induced obesity in mice. Mar. Drugs 15: 90. DOI |
14 |
Chi WJ, Park DY, Seo YB, Chang YK, Lee SY, Hong SK. 2014. Cloning, expression, and biochemical characterization of a novel GH16 |
15 |
Hsu PH, Wei CH, Lu WJ, Shen F, Pan CL, Lin HT. 2015. Extracellular production of a novel endo- |
16 | Chi WJ, Chang YK, Hong SK. 2012. Agar degradation by microorganisms and agar-degrading enzymes. Appl. Microbiol. Biotechnol. 94: 917-930. DOI |
17 |
Belas R. 1989. Sequence analysis of the agrA gene encoding |
18 | Belas R, Bartlett D, Silverman M. 1988. Cloning and gene replacement mutagenesis of a Pseudomonas atlantica agarase gene. Appl. Environ. Microbiol. 54: 30-37. |
19 |
Liao L, Xu XW, Jiang XW, Cao Y, Yi N, Huo YY, et al. 2011. Cloning, expression, and characterization of a new |
20 |
Dong J, Tamaru Y, Araki T. 2007. A unique |
21 | Araki C. 1959. Seaweed polysaccharides, pp. 15-30. In Wolfrom ML (ed.). Carbohydrate Chemistry of Substances of Biological Interests. Pergamon Press, London. |
22 |
Jung S, Lee CR, Chi WJ, Bae CH, Hong SK. 2017. Biochemical characterization of a novel cold-adapted GH39 |
23 |
Potin P, Richard C, Rochas C, Kloareg B. 1993. Purification and characterization of the |
24 |
Day DF, Yaphe W. 1975. Enzymatic hydrolysis of agar: purification and characterization of neoagarobiose hydrolase and p-nitrophenyl |
25 |
Lee DG, Jeon MJ, Lee SH. 2012. Cloning, expression, and characterization of a glycoside hydrolase family 118 |
26 |
Park DY, Chi WJ, Park JS, Chang YK, Hong SK. 2015. Cloning, expression, and biochemical characterization of a GH16 |
27 | Hehemann JH, Correc G, Thomas F, Bernard T, Barbeyron T, Jam M, et al. 2012. Biochemical and structural characterization of the complex agarolytic enzyme system from the marine bacterium Zobellia galactanivorans. J. Biol. Chem. 287: 30571-30584. DOI |
28 |
Cui F, Dong S, Shi X, Zhao X, Zhang XH. 2014. Overexpression and characterization of a novel thermostable |
29 |
Li G, Sun M, Wu J, Ye M, Ge X, Wei W, et al. 2015. Identification and biochemical characterization of a novel endo-type |
30 | Li J, Sha Y, Seswita-Zilda D, Hu Q, He P. 2014. Purification and characterization of thermostable agarase from Bacillus sp. BI-3, a thermophilic bacterium isolated from hot spring. J. Microbiol. Biotechnol. 24: 19-25. DOI |
31 |
Ohta Y, Hatada Y, Miyazaki M, Nogi Y, Ito S, Horikoshi K. 2005. Purification and characterization of a novel |
32 |
Temuujin U, Chi WJ, Chang YK, Hong SK. 2012. Identification and biochemical characterization of Sco3487 from Streptomyces coelicolor A3(2), an exo- and endo-type |
33 |
Temuujin U, Chi WJ, Lee SY, Chang YK, Hong SK. 2011. Overexpression and biochemical characterization of DagA from Streptomyces coelicolor A3(2): an endo-type |
34 |
Zhu Y, Zhao R, Xiao A, Li L, Jiang Z, Chen F, et al. 2016. Characterization of an alkaline |
35 | Tawara M, Sakatoku A, Tiodjio RE, Tanaka D, Nakamura S. 2015. Cloning and characterization of a novel agarase from a newly isolated bacterium Simiduia sp. strain TM-2 able to degrade various seaweeds. Appl. Biochem. Biotechnol. 177: 610-623. DOI |
36 |
Han W, Cheng Y, Wang D, Wang S, Liu H, Gu J, et al. 2016. Biochemical characteristics and substrate degradation pattern of a novel exo-type |
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