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http://dx.doi.org/10.4014/jmb.1806.06050

Comparison of Microbial Diversity and Composition in the Jejunum and Colon of Alcohol-Dependent Rats  

Fan, Yang (Department of Pathogen Biology and Immunology, School of Basic Medical Science, Xi'an Jiaotong University)
Ya-E, Zhao (Department of Pathogen Biology and Immunology, School of Basic Medical Science, Xi'an Jiaotong University)
Ji-dong, Wei (Department of Microbiology, School of Basic Medical Science, Xinxiang Medical University)
Yu-fan, Lu (Department of Microbiology, School of Basic Medical Science, Xinxiang Medical University)
Ying, Zhang (Department of Microbiology, School of Basic Medical Science, Xinxiang Medical University)
Ya-lun, Sun (Department of Microbiology, School of Basic Medical Science, Xinxiang Medical University)
Meng-Yu, Ma (Department of Microbiology, School of Basic Medical Science, Xinxiang Medical University)
Rui-ling, Zhang (The Second Affiliated Hospital, Xinxiang Medical University)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.11, 2018 , pp. 1883-1895 More about this Journal
Abstract
Alcohol dependence is a global public health problem, yet the mechanisms of alcohol dependence are incompletely understood. The traditional view has been that ethanol alters various neurotransmitters and their receptors in the brain and causes the addiction. However, an increasing amount of experimental evidence suggests that gut microbiota also influence brain functions via gut-to-brain interactions, and may therefore induce the development of alcohol use disorders. In this study, a rat model of alcohol dependence and withdrawal was employed, the gut microbiota composition was analyzed by high-throughput 16S rRNA gene sequencing, and the metagenome function was predicted by PICRUSt software. The results suggested that chronic alcohol consumption did not significantly alter the diversity and richness of gut microbiota in the jejunum and colon, but rather markedly changed the microbiota composition structure in the colon. The phyla Bacteroidetes and eight genera including Bacteroidales S24-7, Ruminococcaceae, Parabacteroides, Butyricimonas, et al were drastically increased, however the genus Lactobacillus and gauvreauii in the colon were significantly decreased in the alcohol dependence group compared with the withdrawal and control groups. The microbial functional prediction analysis revealed that the proportions of amino acid metabolism, polyketide sugar unit biosynthesis and peroxisome were significantly increased in the AD group. This study demonstrated that chronic alcohol consumption has a dramatic effect on the microbiota composition structure in the colon but few effects on the jejunum. Inducement of colonic microbiota dysbiosis due to alcohol abuse seems to be a factor of alcohol dependence, which suggests that modulating colonic microbiota composition might be a potentially new target for treating alcohol addiction.
Keywords
Alcohol dependence; gut microbiota; dysbiosis; jejunum; colon;
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