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http://dx.doi.org/10.4014/jmb.1704.04001

Inhibitory Effects of Flavonoids from Spatholobus suberectus on Sortase A and Sortase A-Mediated Aggregation of Streptococcus mutans  

Park, Wanki (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Ahn, Chan-Hong (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Cho, Hyunjoo (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Kim, Chang-Kwon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Shin, Jongheon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Oh, Ki-Bong (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.27, no.8, 2017 , pp. 1457-1460 More about this Journal
Abstract
Seven flavonoids were isolated from Spatholobus suberectus via repetitive column chromatography and high-performance liquid chromatography. The chemical structures of these compounds were identified by spectroscopic analysis and comparison with values reported in the literature. Among the flavonoids tested, 7-hydroxy-6-methoxyflavanone (1) and formononetin (4) exhibited strong inhibitory activity against Streptococcus mutans SrtA, with $IC_{50}$ values of 46.1 and $41.8{\mu}M$, respectively, but did not affect cell viability. The onset and magnitude of inhibition of saliva-induced aggregation in S. mutans treated with compounds 1 and 4 were comparable to the behavior of a srtA-deletion mutant without treatment.
Keywords
Spatholobus suberectus; flavonoids; Streptococcus mutans; sortase A; cell aggregation;
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