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http://dx.doi.org/10.4014/jmb.1702.02050

ROS Scavenging and Anti-Wrinkle Effects of Clitocybin A Isolated from the Mycelium of the Mushroom Clitocybe aurantiaca  

Lee, Joo-Eun (National Research Laboratory for Cardiovascular Stem Cell, Seoul National University College of Medicine and Department of Internal Medicine, Seoul National University Hospital)
Lee, Ik-Soo (KM Convergence Research Division, Korea Institute of Oriental Medicine)
Kim, Kwan-Chul (Innoskin Co., Ltd. BVC, Korea Research Institute of Bioscience and Biotechnology)
Yoo, Ick-Dong (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Yang, Han-Mo (National Research Laboratory for Cardiovascular Stem Cell, Seoul National University College of Medicine and Department of Internal Medicine, Seoul National University Hospital)
Publication Information
Journal of Microbiology and Biotechnology / v.27, no.5, 2017 , pp. 933-938 More about this Journal
Abstract
Clitocybin A, an isoindolinone from Clitocybe aurantiaca, was investigated to assess its anti-wrinkle properties, through reactive oxygen species (ROS)-scavenging and elastase inhibitory activities, procollagen synthesis, and matrix metalloproteinase-1 (MMP-1) expression, in human primary dermal fibroblast-neonatal (HDF-N) cells. Clitocybin A exhibited no significant cytotoxicity up to 10 ppm in HDF-N cells, with cell viability and cell proliferation activity greater than 94.6% and 91.9%, respectively. Strong and concentration-dependent ROS radical scavenging activities of clitocybin A were observed following irradiation with UVB at $30mJ/cm^2$. Furthermore, clitocybin A treatment of cells at 0.1, 1, and 10 ppm exhibited decreased elastase activity, in a concentration-dependent manner, by 1.97%, 6.6%, and 8.31%, respectively, versus the control group. The effects of clitocybin A on procollagen synthesis and MMP-1 expression were investigated. Clitocybin A treatment of cells at 1, 5, and 10 ppm increased procollagen synthesis, by 67.9%, 74.4%, and 112.9%, respectively, versus the control group. At these concentrations, MMP-1 expression decreased significantly following UV irradiation. Together, these findings suggest that clitocybin A may be an effective ingredient for use in anti-wrinkle cosmetic products.
Keywords
Clitocybin A; anti-wrinkle; reactive oxygen species; elastase; procollagen synthesis; matrix metalloproteinase;
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