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http://dx.doi.org/10.4014/jmb.1707.07073

A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase  

Lee, Jin-Moo (Department of Applied Chemistry, Kookmin University)
Cho, Jin-Beom (Department of Applied Chemistry, Kookmin University)
Ahn, Hee-Chul (Department of Pharmacy, Dongguk University-Seoul)
Jung, Woong (Department of Emergency Medicine, Kyung Hee University Hospital at Gangdong)
Jeong, Yong-Joo (Department of Applied Chemistry, Kookmin University)
Publication Information
Journal of Microbiology and Biotechnology / v.27, no.11, 2017 , pp. 2070-2073 More about this Journal
Abstract
We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-N-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained $IC_{50}$ values of $2.09{\pm}0.30{\mu}M$ (ATP hydrolysis) and $13.2{\pm}0.9{\mu}M$ (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when $40{\mu}M$ of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.
Keywords
SARS; helicase; ATP hydrolysis; dsDNA unwinding; cytotoxicity;
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