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http://dx.doi.org/10.4014/jmb.1706.06028

Antioxidative and Antiaging Activities and Component Analysis of Lespedeza cuneata G. Don Extracts Fermented with Lactobacillus pentosus  

Seong, Joon Seob (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology)
Xuan, Song Hua (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology)
Park, So Hyun (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology)
Lee, Keon Soo (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology)
Park, Young Min (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology)
Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology)
Publication Information
Journal of Microbiology and Biotechnology / v.27, no.11, 2017 , pp. 1961-1970 More about this Journal
Abstract
Lespedeza cuneata G. Don is a traditional herb that has been associated with multiple biological activities. In this study, we investigated the antioxidative/antiaging activities and performed an active component analysis of the non-fermented and fermented (using Lactobacillus pentosus) extracts of Lespedeza cuneata G. Don. The antioxidative activities of the fermented extract were higher than those of non-fermented extracts. The elastase inhibitory activity, inhibitory effects on UV-induced MMP-1 expression, and ability to promote type I procollagen synthesis were investigated in Hs68 human fibroblasts cells. These tests also revealed that the fermented extract had increased antiaging activities compared with the non-fermented extract. A component analysis of the ethyl acetate fractions of non-fermented and fermented extracts was performed using TLC, HPLC, and LC/ESI-MS/MS to observe changes in the components before and after fermentation. Six components that were different before and after fermentation were investigated. It was thought that kaempferol and quercetin were converted from kaempferol glucosides and quercetin glucosides, respectively, via bioconversion with the fermentation strain. These results indicate that the fermented extract of L. cuneata G. Don has potential for use as a natural cosmetic material with antioxidative and antiaging effects.
Keywords
Lespedeza cuneata G. Don; Lactobacillus pentosus; fermentation; antioxidative activity; antiaging activity;
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1 Kim SH, Min JW, Quan LH, Lee S, Yang DU, Yang DC. 2012. Enzymatic transformation of ginsenoside Rb1 by Lactobacillus pentosus strain 6105 from kimchi. J. Ginseng Res. 36: 291-297.   DOI
2 Park SN, Won DH, Hwang JP, Han SB. 2014. Cellular protective effects of dehydroeffusol isolated from Juncus effusus L. and the mechanisms underlying these effects. J. Ind. Eng. Chem. 20: 3046-3052.   DOI
3 Tsuji N, Moriwaki S, Suzuki Y, Takema Y, Imokawa G. 2001. The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem. Photobiol. 74: 283-290.   DOI
4 Tsukahara K, Takema Y, Moriwaki S, Tsuji N, Suzuki Y, Fujimura T, et al. 2001. Selective inhibition of skin fibroblast elastase elicits a concentration-dependent prevention of ultraviolet B-induced wrinkle formation. J. Invest. Dermatol. 117: 671-677.   DOI
5 Pittayapruek P, Meephansan J, Prapapan O, Komine M, Ohtsuki M. 2016. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int. J. Mol. Sci. 17: 868-887.   DOI
6 Waridel P, Wolfender JL, Ndjoko K, Hobby KR, Major HJ, Hostettmann K. 2001. Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers. J. Chromatogr. A 926: 29-41.   DOI
7 Sanchez-Rabaneda F, Jauregui O, Lamuela-Raventos RM, Bastida J, Viladomat F, Codina C. 2003. Identification of phenolic compounds in artichoke waste by high-performance liquid chromatography-tandem mass spectrometry. J. Chromatogr. A 1008: 57-72.   DOI
8 Ren Q, Wu C, Ren Y, Zhang J. 2013. Characterization and identification of the chemical constituents from tartary buckwheat (Fagopyrum tataricum Gaertn) by high performance liquid chromatography/photodiode array detector/linear ion trap FTICR hybrid mass spectrometry. Food Chem. 136:1377-1389.   DOI
9 Wang L, Wei W, Tian X, Shi K, Wu Z. 2016. Improving bioactivities of polyphenol extracts from Psidium guajava L. leaves through co-fermentation of Monascus anka GIM 3.592 and Saccharomyces cerevisiae GIM 2.139. Ind. Crops Prod. 94: 206-215.   DOI
10 Kim JE, Kim WY, Kim JW, Park HS, Lee SH, Lee SY, et al. 2010. Antibacterial, antioxidative activity and component analysis of Pinus koraiensis leaf extracts. J. Soc. Cosmet. Sci. Korea 36: 303-314.
11 Lee NK, Paik HD. 2017. Bioconversion using lactic acid bacteria: ginsenosides, GABA, and phenolic compounds. J. Microbiol. Biotechnol. 27: 869-877.   DOI
12 Okabe Y, Shimazu T, Tanimoto H. 2011. Higher bioavailability of isoflavones after a single ingestion of aglycone-rich fermented soybeans compared with glucoside-rich nonfermented soybeans in Japanese postmenopausal women. J. Sci. Food Agric. 91: 658-663.   DOI
13 Schmidt CG, Goncalves LM, Prietto L, Hackbart HS, Furlong EB. 2014. Antioxidant activity and enzyme inhibition of phenolic acids from fermented rice bran with fungus Rhizopus oryzae. Food Chem. 146: 371-377.   DOI
14 Park BG, Jung HJ, Cho YW, Lim HW, Lim CJ. 2013. Potentiation of antioxidative and anti-inflammatory properties of cultured wild ginseng root extract through probiotic fermentation. J. Pharm. Pharmacol. 65: 457-464.   DOI
15 Yang HJ, Kim EH, Park JO, Kim JE, Park SN. 2009. Antioxidative activity and component analysis of fermented Melissa officinalis extracts. J. Soc. Cosmet. Sci. Korea 35: 47-55.
16 Ahn YJ, Won BR, Kang MK, Kim JH, Park SN. 2009. Antioxidant activity and component analysis of fermented Lavandula angustifolia extracts. J. Soc. Cosmet. Sci. Korea 35: 125-134.
17 Im AR, Song JH, Lee MY, Yeon SH, Um KA, Chae S. 2014. Anti-wrinkle effects of fermented and non-fermented Cyclopia intermedia in hairless mice. BMC Complement. Altern. Med. 14: 424-429.   DOI
18 Jeon SM, Kim SI, Ahn JY, Park SN. 2007. Antioxidative properties of extract/fractions of Suaeda asparagoides and Salicornia herbacea extracts (I). J. Soc. Cosmet. Sci. Korea 33:145-152.
19 De Leo M, Abreu MBD, Pawlowska AM, Cioni PL, Braca A. 2010. Profiling the chemical content of Opuntia ficus-indica flowers by HPLC-PDA-ESI-MS and GC/EIMS analyses. Phytochem. Lett. 3: 48-52.   DOI
20 Yoo G, Park SJ, Lee TH, Yang H, Baek YS, Kim N, et al. 2015. Flavonoids isolated from Lespedeza cuneata G. Don and their inhibitory effects on nitric oxide production in lipopolysaccharide-stimulated BV-2 microglia cells. Pharmacogn. Mag. 11: 651-656.   DOI
21 Kim DH, Kim JH, Baek SH, Seo JH, Kho YH, Oh TK, et al. 2004. Enhancement of tyrosinase inhibition of the extract of Veratrum patulum using cellulase. Biotechnol. Bioeng. 87: 849-854.   DOI
22 Chiang HM, Chen HC, Lin TJ, Shih IC, Wen KC. 2012. Michelia alba extract attenuates UVB-induced expression of matrix metalloproteinases via MAP kinase pathway in human dermal fibroblasts. Food Chem. Toxicol. 50: 4260-4269.   DOI
23 Kim YH, Ryu SN. 2008. Antioxidant activity of methanol extract from aerial parts in Lespedeza cuneata G. Don. Korean J. Crop Sci. 53: 121-123.
24 Tsai CC, Chan CF, Huang WY, Lin JS, Chan P, Liu HY, et al. 2013. Applications of Lactobacillus rhamnosus spent culture supernatant in cosmetic antioxidation, whitening and moisture retention applications. Molecules 18: 14161-14171.   DOI
25 Kammeyer A, Luiten RM. 2015. Oxidation events and skin aging. Ageing Res. Rev. 21: 16-29.   DOI
26 Kim M, Park YG, Lee HJ, Lim SJ, Nho CW. 2015. Youngiasides A and C isolated from Youngia denticulatum inhibit UVBinduced MMP expression and promote type I procollagen production via repression of MAPK/AP-1/NF-${\kappa}B$ and activation of AMPK/Nrf2 in HaCaT cells and human dermal fibroblasts. J. Agric. Food Chem. 63: 5428-5438.   DOI
27 Kwon DJ, Kim JK, Ham YH, Bae YS. 2007. Flavone glycosides from the aerial parts of Lespedeza cuneata G. Don. J. Korean Soc. Appl. Biol. Chem. 50: 344-347.
28 Lee HJ, Lim GN, Park MA, Park SN. 2011. Antibacterial and antioxidative activity of Lespedeza cuneata G. Don extracts. Korean J. Microbiol. Biotechnol. 39: 63-69.
29 Ridwan BU, Koning CJ, Besselink MG, Timmerman HM, Brouwer EC, Verhoef J, et al. 2008. Antimicrobial activity of a multispecies probiotic (Ecologic 641) against pathogens isolated from infected pancreatic necrosis. Lett. Appl. Microbiol. 46: 61-67.
30 Quan LH, Kim YJ, Li GH, Choi KT, Yang DC. 2013. Microbial transformation of ginsenoside Rb1 to compound K by Lactobacillus paralimentarius. World J. Microbiol. Biotechnol. 29: 1001-1007.   DOI
31 Pyo YH, Lee TC, Lee YC. 2005. Enrichment of bioactive isoflavones in soymilk fermented with ${\beta}$-glucosidase-producing lactic acid bacteria. Food Res. Int. 38: 551-559.   DOI
32 Huynh TN, Smagghe G, Gonzales GB, Camp JV, Raes K. 2016. Extraction and bioconversion of kaempferol metabolites from cauliflower outer leaves through fungal fermentation. Biochem. Eng. J. 116: 27-33.   DOI
33 Min JW, Kim HJ, Joo KS, Kang HC. 2015. Isolation of Stenotrophomonas rhizopilae strain GFC09 with ginsenoside converting activity and anti-wrinkle effects of converted ginsenosides. J. Soc. Cosmet. Sci. Korea 41: 375-382.