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http://dx.doi.org/10.4014/jmb.1604.04016

Pathways Regulating the pbgP Operon and Colistin Resistance in Klebsiella pneumoniae Strains  

Choi, Myung-Jin (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine)
Kim, Sunju (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine)
Ko, Kwan Soo (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.9, 2016 , pp. 1620-1628 More about this Journal
Abstract
In this study, we investigated colistin resistance mechanisms associated with the regulation of the pbgP operon in Klebsiella pneumoniae, using four isogenic pairs of colistin-susceptible strains and their colistin-resistant derivatives and two colistin-resistant clinical isolates. Amino acid sequence alterations of PhoPQ, PmrAB, and MgrB were investigated, and mRNA expression levels of phoQ, pmrB, pmrD, and pbgP were measured using quantitative real-time PCR. The phoQ and pmrB genes were deleted from two colistin-resistant derivatives, 134R and 063R. We found that phoQ, pmrD, and pbgP were significantly upregulated in all colistin-resistant derivatives. However, pmrB was significantly upregulated in only two colistin-resistant derivatives and one clinical strain. pmrB was not overexpressed in the other strains. The minimum inhibitory concentration of colistin was drastically lower in both phoQ- and pmrB-deleted mutants from a colistin-resistant derivative (134R) that was overexpressing phoQ and pmrB. However, colistin susceptibility was restored only in a phoQ-deleted mutant from a colistin-resistant derivative (063R) without overexpression of pmrB. In conclusion, two different regulations of the pbgP operon may associate with the development of colistinresisant K. pneumoniae.
Keywords
Colistin resistance; Klebsiella pneumoniae; two-component regulatory systems;
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