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http://dx.doi.org/10.4014/jmb.1601.01044

Differential Cytokine Regulatory Effect of Three Lactobacillus Strains Isolated from Fermented Foods  

Lee, Yoon-Doo (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
Hong, Yi-Fan (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
Jeon, Boram (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
Jung, Bong Jun (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
Chung, Dae Kyun (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
Kim, Hangeun (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.9, 2016 , pp. 1517-1526 More about this Journal
Abstract
Lactic acid bacteria (LAB) isolated from fermented foods have potential as a treatment for immune-related disorders and the use of LAB has been increasing worldwide. In this study, the differential cytokine regulatory effect was examined with three isolates of lactobacilli strains; namely, Lactobacillus plantarum K55-5 isolated from dairy product, and L. sakei K101 and L. plantarum K8 previously isolated from kimchi (a Korean traditional fermented vegetable). Production of cytokines such as IL-10, IL-12, IFN-γ, and TNF-α was significantly increased in L. sakei K101- and L. plantarum K55-5-treated splenocytes as compared with controls. The oral administration of L. sakei K101 and L. plantarum K55-5 increased cytokine production in the immunosuppressed mouse splenocytes and blood. NK cell cytotoxic activity was also increased in L. sakei K101- and L. plantarum K55-5-fed mice. On the other hand, L. plantarum K8 did not affect cytokine induction in all the experiments performed in this study. The cytokine-inducing effect of L. plantarum K55-5 was significantly increased by lysates of heat-killed bacteria as compared with live, heat-killed, or supernatant of cell lysates. TNF-α production by lipoteichoic acids (LTAs) isolated from the three isolates of lactobacilli was compared, and it was found that K55-5 LTA had a highest cytokine-inducing ability, which was mediated by TLR2-mediated NF-κB and ERK activation. Taken together, our study suggests that L. plantarum K55-5 and L. sakei K101 can be used for the treatment of immunosuppressed disorders.
Keywords
Lactobacillus plantarum; Lactobacillus sakei; cytokine; immunosuppression; inflammation; lipoteichoic acid;
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1 Kim HG, Gim MG, Kim JY, Hwang HJ, Ham MS, Lee JM, et al. 2007. Lipoteichoic acid from Lactobacillus plantarum elicits both the production of interleukin-23p19 and suppression of pathogen mediated interleukin-10 in THP-1 cells. FEMS Immunol. Med. Microbiol. 49: 205-214.   DOI
2 Kaila M, Isolauri E, Soppi E, Virtanen E, Laine S, Arvilommi H. 1992. Enhancement of the circulating antibody secreting cell response in human diarrhea by a human Lactobacillus strain. Pediatr. Res. 32: 141-144.   DOI
3 Kandler O, Weiss N. 1986. Regular, non-sporing, gram positive rods, pp. 1208-1234. In Sneath DHA, Mair NC, Sharpe ME, Holt JH (eds.). Bergey's Manual of Systematic Bacteriology, Vol. 2. Williams and Wilkins, New York.
4 Kassayová M, Bobrov N, Strojný L, Kisková T, Mikeš J, Deme ková V, et al. 2014. Preventive effects of probiotic bacteria Lactobacillus plantarum and dietary fiber in chemically-induced mammary carcinogenesis. Anticancer Res. 34: 4969-4975.
5 Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, et al. 2008. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J. Immunol. 180: 2553-2561.   DOI
6 Kim JY, Kim H, Jung BJ, Kim NR, Park JE, Chung DK. 2013. Lipoteichoic acid isolated from Lactobacillus plantarum suppresses LPS-mediated atherosclerotic plaque inflammation. Mol. Cells 35: 115-124.   DOI
7 Locksley RM, Killeen N, Lenardo MJ. 2001. The TNF and TNF receptor superfamilies: integrating mammalian biology. Cell 104: 487-501.   DOI
8 Lomax AR, Calder PC. 2009. Probiotics, immune function, infection and inflammation: a review of the evidence from studies conducted in humans. Curr. Pharm. Des. 15: 1428-1518.   DOI
9 Ouwehand AC, Salminen S, Isolauri E. 2002. Probiotics: an overview of beneficial effects. Antonie Van Leeuwenhoek 82: 279-289.   DOI
10 Morath S, Geyer A, Hartung T. 2001. Structure-function relationship of cytokine induction by lipoteichoic acid from Staphylococcus aureus. J. Exp. Med. 193: 393-397.   DOI
11 Noh SY, Kang SS, Yun CH, Han SH. 2015. Lipoteichoic acid from Lactobacillus plantarum inhibits Pam2CSK4-induced IL-8 production in human intestinal epithelial cells. Mol. Immunol. 64: 183-189.   DOI
12 Ohshima Y, Beuth J, Yassin A, Ko HL, Pulverer G. 1988. Stimulation of human monocyte chemiluminescence by staphylococcal lipoteichoic acid. Med. Microbiol. Immunol. 177: 115-121.
13 Ouyang W, Rutz S, Crellin NK, Valdez PA, Hymowitz SG. 2011. Regulation and functions of the IL-10 family of cytokines in inflammation and disease. Annu. Rev. Immunol. 29: 71-109.   DOI
14 Perdigón G, Alvarez S, Pesce de Ruiz Holgado A. 1991. Immunoadjuvant activity of oral Lactobacillus casei: influence of dose on the secretory immune response and protective capacity in intestinal infections. J. Dairy Res. 58: 485-496.   DOI
15 Ryu YH, Baik JE, Yang JS, Kang SS, Im J, Yun CH, et al. 2009. Differential immune-stimulatory effects of gram-positive bacteria due to their lipoteichoic acids. Int. Immunopharmacol. 9: 127-133.   DOI
16 Saez-Lara MJ, Gomez-Llorente C, Plaza-Diaz J, Gil A. 2015. The role of probiotic lactic acid bacteria and bifidobacteria in the prevention and treatment of inflammatory bowel disease and other related diseases: a systematic review of randomized human clinical trials. Biomed. Res. Int. 2015: 505878.   DOI
17 Vivier E, Tomasello E, Baratin M, Walzer T, Ugolini S. 2008. Functions of natural killer cells. Nat. Immunol. 9: 503-510.   DOI
18 Tsai YT, Cheng PC, Pan TM. 2012. The immunomodulatory effects of lactic acid bacteria for improving immune functions and benefits. Appl. Microbiol. Biotechnol. 96: 853-862.   DOI
19 Satish Kumar CS, Kondal Reddy K, Reddy AG, Vinoth A, Ch SR, Boobalan G, Rao GS. 2015. Protective effect of Lactobacillus plantarum 21, a probiotic on trinitrobenzenesulfonic acid-induced ulcerative colitis in rats. Int. Immunopharmacol. 25: 504-510.   DOI
20 Toumi R, Soufli I, Rafa H, Belkhelfa M, Biad A, Touil-Boukoffa C. 2014. Probiotic bacteria Lactobacillus and Bifidobacterium attenuate inflammation in dextran sulfate sodium-induced experimental colitis in mice. Int. J. Immunopathol. Pharmacol. 27: 615-627.   DOI
21 Wang IK, Wu YY, Yang YF, Ting IW, Lin CC, Yen TH, et al. 2015. The effect of probiotics on serum levels of cytokine and endotoxin in peritoneal dialysis patients: a randomised, double-blind, placebo-controlled trial. Benef. Microbes 21: 1-8.
22 Won TJ, Kim B, Song DS, Lim YT, Oh ES, Lee DI, et al. 2011. Modulation of Th1/Th2 balance by Lactobacillus strains isolated from kimchi via stimulation of macrophage cell line J774A.1 in vitro. J. Food Sci. 76: H55-H61.   DOI
23 Finegold SM, Attebery HR, Sutter VL. 1974. Effect of diet on human faecal flora: comparison of Japanese and American diets. Am. J. Clin. Nutr. 27: 1546-1569.
24 Abel K, La Franco-Scheuch L, Rourke T, Ma ZM, De Silva V, Fallert B, et al. 2004. Gamma interferon-mediated inflammation is associated with lack of protection from intravaginal simian immunodeficiency virus SIVmac239 challenge in simian-human immunodeficiency virus 89.6-immunized rhesus macaques. J. Virol. 78: 841-854.   DOI
25 Adlerberth I, Ahrne S, Johansson ML, Molin G, Hanson LA, Wold AE. 1996. A mannose-specific adherence mechanism in Lactobacillus plantarum conferring binding to the human colonic cell line HT-29. Appl. Environ. Microbiol. 62: 2244-2251.
26 Gee K, Guzzo C, Che Mat NF, Ma W, Kumar A. 2009. The IL-12 family of cytokines in infection, inflammation and autoimmune disorders. Inflamm. Allergy Drug Targets 8: 40-52.   DOI
27 Amdekar S, Singh V, Kumar A, Sharma P, Singh R. 2014. Lactobacillus acidophilus protected organs in experimental arthritis by regulating the pro-inflammatory cytokines. Indian J. Clin. Biochem. 29: 471-478.   DOI
28 Cross ML, Gill HS. 2001. Can immunoregulatory lactic acid bacteria be used as dietary supplements to limit allergies? Int. Arch. Allergy Immunol. 125: 112-119.   DOI
29 Hong YF, Kim H, Kim HR, Gim MG, Chung DK. 2014. Different immune regulatory potential of Lactobacillus plantarum and Lactobacillus sakei isolated from kimchi. J. Microbiol. Biotechnol. 24: 1629-1635.   DOI
30 Jang KS, Baik JE, Han SH, Chung DK, Kim BG. 2011. Multi-spectrometric analyses of lipoteichoic acids isolated from Lactobacillus plantarum. Biochem. Biophys. Res. Commun. 407: 823-830.   DOI
31 Jensen H, Drømtorp SM, Axelsson L, Grimmer S. 2015. Immunomodulation of monocytes by probiotic and selected lactic acid bacteria. Probiotics Antimicrob. Proteins 7: 14-23.   DOI
32 Jeong JH, Jang S, Jung BJ, Jang KS, Kim BG, Chung DK, Kim H. 2015. Differential immune-stimulatory effects of LTAs from different lactic acid bacteria via MAPK signaling pathway in RAW 264.7 cells. Immunobiology 220: 460-466.   DOI
33 Johansson ML, Molin G, Jeppsson B, Nobaek S, Ahrné S, Bengmark S. 1993. Administration of different Lactobacillus strains in fermented oatmeal soup: in vivo colonization of human intestinal mucosa and effect on the indigenous flora. Appl. Environ. Microbiol. 59: 15-20.