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http://dx.doi.org/10.4014/jmb.1604.04049

Persistence of Multidrug-Resistant Acinetobacter baumannii Isolates Harboring blaOXA-23 and bap for 5 Years  

Sung, Ji Youn (Department of Biomedical Laboratory Science, Far East University)
Koo, Sun Hoe (Department of Laboratory Medicine, College of Medicine, Chungnam National University)
Kim, Semi (Department of Laboratory Medicine, College of Medicine, Chungnam National University)
Kwon, Gye Cheol (Department of Laboratory Medicine, College of Medicine, Chungnam National University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.8, 2016 , pp. 1481-1489 More about this Journal
Abstract
The emergence and dissemination of carbapenemase-producing Acinetobacter baumannii isolates have been reported worldwide, and A. baumannii isolates harboring blaOXA-23 are often resistant to various antimicrobial agents. Antimicrobial resistance can be particularly strong for biofilm-forming A. baumannii isolates. We investigated the genetic basis for carbapenem resistance and biofilm-forming ability of multidrug-resistant (MDR) clinical isolates. Ninety-two MDR A. baumannii isolates were collected from one university hospital located in the Chungcheong area of Korea over a 5-year period. Multiplex PCR and DNA sequencing were performed to characterize carbapenemase and bap genes. Clonal characteristics were analyzed using REP-PCR. In addition, imaging and quantification of biofilms were performed using a crystal violet assay. All 92 MDR A. baumannii isolates involved in our study contained the blaOXA-23 and bap genes. The average absorbance of biomass in Bap-producing strains was much greater than that in non-Bap-producing strains. In our study, only three REP-PCR types were found, and the isolates showing type A or type B were found more than 60 times among unique patients during the 5 years of surveillance. These results suggest that the isolates have persisted and colonized for 5 years, and biofilm formation ability has been responsible for their persistence and colonization.
Keywords
Carbapenemase; Acinetobacter baumannii; antimicrobial resistance; biofilm; crystal violet assay;
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