Browse > Article
http://dx.doi.org/10.4014/jmb.1602.02028

Curcumin Blocks Naproxen-Induced Gastric Antral Ulcerations through Inhibition of Lipid Peroxidation and Activation of Enzymatic Scavengers in Rats  

Kim, Jeong-Hwan (Blue-Bio Industry RIC, Dong-Eui University)
Jin, Soojung (Blue-Bio Industry RIC, Dong-Eui University)
Kwon, Hyun Ju (Blue-Bio Industry RIC, Dong-Eui University)
Kim, Byung Woo (Blue-Bio Industry RIC, Dong-Eui University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.8, 2016 , pp. 1392-1397 More about this Journal
Abstract
Curcumin is a polyphenol derived from the plant Curcuma longa, which is used for the treatment of diseases associated with oxidative stress and inflammation. The present study was undertaken to determine the protective effect of curcumin against naproxen-induced gastric antral ulcerations in rats. Different doses (10, 50, and 100 mg/kg) of curcumin or vehicle (curcumin, 0 mg/kg) were pretreated for 3 days by oral gavage, and then gastric mucosal lesions were caused by 80 mg/kg naproxen applied for 3 days. Curcumin significantly inhibited the naproxen-induced gastric antral ulcer area and lipid peroxidation in a dose-dependent manner. In addition, curcumin markedly increased activities of radical scavenging enzymes, such as superoxide dismutase (SOD), catalase, and glutathione peroxidase in a dose-dependent manner. Specifically, 100 mg/kg curcumin completely protected the gastric mucosa against the loss in the enzyme, resulting in a drastic increase of activities of radical scavenging enzymes up to more than the level of untreated normal rats. Histological examination obviously showed that curcumin prevents naproxen-induced gastric antral ulceration as a result of direct protection of the gastric mucosa. These results suggest that curcumin blocks naproxen-induced gastric antral ulcerations through prevention of lipid peroxidation and activation of radical scavenging enzymes, and it may offer a potential remedy of gastric antral ulcerations.
Keywords
Curcumin; gastric antral ulceration; lipid peroxidation; naproxen; oral gavage; radical scavenging enzymes;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Aebi H. 1974. In Bergmeyer HU (ed.). Methods of Enzymatic Analysis, pp. 674-678. Academic Press, New York.
2 Ajaikumar BK, Sushovan G, Sunil K, Parmeswaran D, Juri G, Bharat BA. 2007. Curcumin potentiates antitumor activity of gemcitabine in an orthotopic model of pancreatic cancer through suppression of proliferation, angiogenesis, and inhibition of nuclear factor-κB-regulated gene products. Cancer Res. 67: 3853-3861.   DOI
3 Beck WS, Schneider HT, Dietzel K, Nuernberg B, Brune K. 1990. Gastrointestinal ulcerations induced by anti-inflammatory drugs in rats. Arch. Toxicol. 64: 210-217.   DOI
4 Calhoun W, Gilman SC, Datko LJ, Copenhaver TW, Carlson RP. 1992. Interaction studies of tilomisole, aspirin and naproxen in acute and chronic inflammation with assessment of gastrointestinal irritancy in the rat. Agents Actions 36: 99-106.   DOI
5 Cioli V, Putzolu S, Rossi V, Scorza BP, Corradino C. 1979. The role of direct tissue contact in the production of gastrointestinal ulcers by anti-inflammatory drugs in rats. Toxicol. Appl. Pharmacol. 50: 283-289.   DOI
6 Glienke W, Maute L, Wicht J, Bergmann L. 2009. Wilms' tumour gene 1 (WT1) as a target in curcumin treatment of pancreatic cancer cells. Eur. J. Cancer 45: 874-880.   DOI
7 Jiao Y, Wilkinson J 4th, Di X, Wang W, Hatcher H, Kock ND, et al. 2009. Curcumin, a cancer chemopreventive and chemotherapeutic agent, is a biologically active iron chelator. Blood 113: 462-469.   DOI
8 Half E, Arber N. 2009. Colon cancer: preventive agents and the present status of chemoprevention. Expert Opin. Pharmacother. 10: 211-219.   DOI
9 Hogeboom GH. 1955. In Colowick SP, Kaplan NO (eds.). Methods in Enzymology, pp. 16-19. Academic Press, New York.
10 Huang MT, Lysz T, Ferraro T, Abidi TF, Laskin JD, Conney AH. 1991. Inhibitory effects of curcumin on in vitro lipoxygenase and cyclooxygenase activities in mouse epidermis. Cancer Res. 51: 813-819.
11 Kang J, Chen J, Shi Y, Jia J, Zhang Y. 2005. Curcumininduced histone hypoacetylation: the role of reactive oxygen species. Biochem. Pharmacol. 69: 1205-1213.   DOI
12 Kim JH, Kim YS, Song GG, Park JJ, Chang HI. 2005. Protective effect of astaxanthin on naproxen-induced gastric antral ulceration in rats. Eur. J. Pharmacol. 514: 53-59.   DOI
13 Krishnan MD, Virendra BM, Darrell WB. 2007. Curcumin suppresses growth and chemoresistance of human glioblastoma cells via AP-1 and NF-κB transcription factors. J. Neurochem. 102: 522-538.   DOI
14 Lawrence RA, Burk RF. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun. 71: 952-958.   DOI
15 Lin YG, Kunnumakkara AB, Nair A, Merritt WM, Han LY, Armaiz-Pena GN, et al. 2007. Curcumin inhibits tumor growth and angiogenesis in ovarian carcinoma by targeting the nuclear factor-κB pathway. Clin. Cancer. Res. 13: 3423-3430.   DOI
16 McCarthy DM. 1995. Mechanisms of mucosal injury and healing: the role of nonsteroidal anti-inflammatory drugs. Scand. J. Gastroenterol. 208: 24-29.   DOI
17 Liu E, Wu J, Cao W, Zhang J, Liu W, Jiang X, Zhang X. 2007. Curcumin induces G2/M cell cycle arrest in a p53-dependent manner and upregulates ING4 expression in human glioma. J. Neurooncol. 85: 263-270.   DOI
18 Lodha R, Bagga A. 2000. Traditional Indian systems of medicine. Ann. Acad. Med. Singapore 29: 37-41.
19 McCord JM, Fridovich I. 1967. Superoxide dismutase, an enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244: 6049-6055.
20 McCarthy DM. 1990. NSAID-induced gastro-intestinal damage - a critical review of prophylaxis and therapy. J. Clin. Gastroenterol. 12: S13-S20.   DOI
21 Milacic V, Banerjee S, Landis-Piwowar KR, Sarkar FH, Majumdar AP, Dou QP. 2008. Curcumin inhibits the proteasome activity in human colon cancer cells in vitro and in vivo. Cancer Res. 68: 7283-7292.   DOI
22 Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95: 351-358.   DOI
23 Parks DA. 1989. Oxygen radicals: mediators of gastrointestinal pathophysiology. Gut 30: 293-298.   DOI
24 Roth SH, Bennett RE. 1987. Non-steroidal anti-inflammatory drug gastropathy: recognition and response. Arch. Intern. Med. 147: 2093-2100.   DOI
25 Ruby AJ, Kuttan G, Babu KD, Rajasekharan KN, Kuttan R. 1995. Anti-tumour and antioxidant activity of natural curcuminoids. Cancer Lett. 94: 79-83.   DOI
26 Surajit K, Naren LB, Swapan KR. 2007. Curcumin suppressed anti-apoptotic signals and activated cysteine proteases for apoptosis in human malignant glioblastoma U87MG cells. Neurochem. Res. 32: 2103-2113.   DOI
27 Salvioli S, Sikora E, Cooper EL, Franceschi C. 2007. Curcumin in cell death processes: a challenge for CAM of age-related pathologies. Evid. Based Complement. Alternat. Med. 4: 181-190.   DOI
28 Sankar B, Debaprasad M, Baisakhi S, Gouri SS, Tanya D, Gaurisankar S. 2007. Curcumin prevents tumor-induced T cell apoptosis through Stat-5a-mediated Bcl-2 induction. J. Biol. Chem. 282: 15954-15964.   DOI
29 Suwa T, Urano H, Kohno Y, Suzuki A, Amano T. 1987. Comparative studies on the gastrointestinal lesions caused by several nonsteroidal anti-inflammatory agents in rats. Agents Actions 21: 167-172.   DOI
30 Shishodia S, Sethi G, Aggarwal BB. 2005. Curcumin: getting back to the roots. Ann. NY Acad. Sci. 1056: 206-217.   DOI
31 Swamy MV, Citineni B, Patlolla JM, Mohammed A, Zhang Y, Rao CV. 2008. Prevention and treatment of pancreatic cancer by curcumin in combination with omega-3 fatty acids. Nutr. Cancer 60: 81-89.   DOI
32 Thiyagarajan M, Sharma SS. 2004. Neuroprotective effect of curcumin in middle cerebral artery occlusion induced focal cerebral ischemia in rats. Life Sci. 74: 969-985.   DOI
33 Wang Q, Sun AY, Simonyi A, Jensen MD, Shelat PB, Rottinghaus GE, et al. 2005. Neuroprotective mechanisms of curcumin against cerebral ischemia-induced neuronal apoptosis and behavioral deficits. J. Neurosci. Res. 82: 138-148.   DOI
34 Yang F, Lim GP, Begum AN, Ubeda OJ, Simmons MR, Ambegaokar SS, et al. 2005. The curry spice curcumin reduces oxidative damage and amyloid pathology in an Alzheimer transgenic mouse. J. Biol. Chem. 280: 5892-5901.   DOI
35 Yoshikawa T, Naito Y, Ueda S, Oyamada H, Takemura T, Yoshida N, et al. 1990. Role of oxygen-derived free radicals in the pathogenesis of gastric mucosal lesions in rats. J. Clin. Gastroenterol. 12: 65-71.   DOI
36 Yu Z, Shah DM. 2007. Curcumin down-regulates Ets-1 and Bcl-2 expression in human endometrial carcinoma HEC-1-A cells. Gynecol. Oncol. 103: 541-548.   DOI
37 Ashish MK, Gautam S, Bharat BA. 2007. Curcumin potentiates the apoptosis effects of chemotherapeutic agents and cytokines through down-regulation of nuclear factor-κB and nuclear factor-κB-regulated gene products in IFN-α-sensitive and IFN-α-resistant human bladder cancer cells. Mol. Cancer Ther. 6: 1022-1030.   DOI