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http://dx.doi.org/10.4014/jmb.1512.12046

Evaluation of Th1/Th2-Related Immune Response against Recombinant Proteins of Brucella abortus Infection in Mice  

Im, Young Bin (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University)
Park, Woo Bin (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University)
Jung, Myunghwan (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University)
Kim, Suk (College of Veterinary Medicine, Gyeongsang National University)
Yoo, Han Sang (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.6, 2016 , pp. 1132-1139 More about this Journal
Abstract
Brucellosis is a zoonotic disease caused by Brucella, a genus of gram-negative bacteria. Cytokines have key roles in the activation of innate and acquired immunities. Despite several research attempts to reveal the immune responses, the mechanism of Brucella infection remains unclear. Therefore, immune responses were analyzed in mice immunized with nine recombinant proteins. Cytokine production profiles were analyzed in the RAW 264.7 cells and naive splenocytes after stimulation with three recombinant proteins, metal-dependent hydrolase (r0628), bacterioferritin (rBfr), and thiamine transporter substrate-binding protein (rTbpA). Immune responses were analyzed by ELISA and ELISpot assay after immunization with proteins in mice. The production levels of NO, TNF-α, and IL-6 were time-dependently increased after having been stimulated with proteins in the RAW 264.7 cells. In naive splenocytes, the production of IFN-γ and IL-2 was increased after stimulation with the proteins. It was concluded that two recombinant proteins, r0628 and rTbpA, showed strong immunogenicity that was induced with Th1-related cytokines IFN-γ, IL-2, and TNF-α more than Th2-related cytokines IL-6, IL-4, and IL-5 in vitro. Conversely, a humoral immune response was activated by increasing the number of antigen-secreting cells specifically. Furthermore, these could be candidate diagnosis antigens for better understanding of brucellosis.
Keywords
Brucella abortus; cytokine; immunogenicity; recombinant protein;
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