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http://dx.doi.org/10.4014/jmb.1512.12041

Cell Surface Display of Four Types of Solanum nigrum Metallothionein on Saccharomyces cerevisiae for Biosorption of Cadmium  

Wei, Qinguo (College of Life Science, Qufu Normal University)
Zhang, Honghai (College of Life Science, Qufu Normal University)
Guo, Dongge (College of Life Science, Qufu Normal University)
Ma, Shisheng (College of Life Science, Qufu Normal University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.5, 2016 , pp. 846-853 More about this Journal
Abstract
We displayed four types of Solanum nigrum metallothionein (SMT) for the first time on the surface of Saccharomyces cerevisiae using an α-agglutinin-based display system. The SMT genes were amplified by RT-PCR. The plasmid pYES2 was used to construct the expression vector. Transformed yeast strains were confirmed by PCR amplification and custom sequencing. Surface-expressed metallothioneins were indirectly indicated by the enhanced cadmium sorption capacity. Flame atomic absorption spectrophotometry was used to examine the concentration of Cd2+ in this study. The transformed yeast strains showed much higher resistance ability to Cd2+ compared with the control. Strikingly, their Cd2+ accumulation was almost twice as much as that of the wild-type yeast cells. Furthermore, surface-engineered yeast strains could effectively adsorb ultra-trace cadmium and accumulate Cd2+ under a wide range of pH levels, from 3 to 7, without disturbing the Cu2+ and Hg2+. Four types of surfaceengineered Saccharomyces cerevisiae strains were constructed and they could be used to purify Cd2+-contaminated water and adsorb ultra-trace cadmium effectively. The surface-engineered Saccharomyces cerevisiae strains would be useful tools for the bioremediation and biosorption of environmental cadmium contaminants.
Keywords
Cadmium; surface display; metallothionein; Solanum nigrum; Saccharomyces cerevisiae; flame atomic absorption spectrophotometry (FAAS);
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1 Akbal F, Camci S. 2012. Treatment of metal plating wastewater by electrocoagulation. Environ. Prog. Sustain. Energy 31: 340-350.   DOI
2 Alexander J, Benford D, Cockburn A, Cravedi JP, Doglioti E, Domenico AD. 2009. Scientific opinion of the panel on contaminants in the food chain on a request from the European Commission on cadmium in food. EFSA J. 980: 1-139.
3 Arief VO, Trilestari K, Sunarso J, Indraswati N, Ismadji S. 2008. Recent progress on biosorption of heavy metals from liquids using low cost biosorbents: characterization, biosorption parameters and mechanism studies. Clean (Weinh) 36: 937-962.
4 Bae W, Chen W, Mulchandani A, Mehra RK. 2000 Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins. Biotechnol. Bioeng. 70: 518-524.   DOI
5 Batayneh AT. 2012. Toxic (aluminum, beryllium, boron, chromium and zinc) in groundwater: health risk assessment. Int. J. Environ. Sci. Technol. 9: 153-162.   DOI
6 Dabrowski A, Hubicki Z, Podkoscielny P, Robens E. 2004. Selective removal of the heavy metal ions from waters and industrial wastewaters by ion-exchange method. Chemosphere 56: 91-106.   DOI
7 Davis TA, Volesky B, Mucci A. 2003 A review of the biochemistry of heavy metal biosorption by brown algae. Water Res. 37: 4311-4330.   DOI
8 Dhankhar R, Hooda A. 2011. Fungal biosorption - an alternative to meet the challenges of heavy metal pollution in aqueous solutions. Environ. Technol. 32: 467-491.   DOI
9 El-Helow HR, Sabry SA, Amer RM. 2000. Cadmium biosorption by a cadmium resistant strain of Bacillus thuringiensis: regulation and optimization of cell surface affinity for metal cations. Biometals 13: 273-280.   DOI
10 Ferraz P, Fidalgo F, Almeid A, Teixeira J. 2012. Phytostabilization of nickel by the zinc and cadmium hyperaccumulator Solanum nigrum L. Are metallothioneins involved? Plant Physiol. Biochem. 57: 254-260.   DOI
11 Gadd GM. 1990. Heavy metal accumulation by bacteria and other microorganisms. Experientia 46: 834-840.   DOI
12 Hammaini A, Ballester A, Blázquez ML, González F, Muñoz J. 2002. Effect of the presence of lead on the biosorption of copper, cadmium and zinc by activated sludge. Hydrometallurgy 67: 109-116.   DOI
13 He XC, Chen WL, Huang QY. 2012. Surface display of monkey metallothionein α tandem repeats and EGFP fusion protein on Pseudomonas putida X4 for biosorption and detection of cadmium. Appl. Microbiol. Biotechnol. 95: 1605-1613.   DOI
14 Holan ZR, Volesky B, Prasetyo I. 1993. Biosorption of cadmium by biomass of marine algae. Biotechnol. Bioeng. 41: 819-825.   DOI
15 Kotrba P, Ruml T. 2010. Surface display of metal fixation motifs of bacterial P1-type ATPases specifically promotes biosorption of Pb2+ by Saccharomyces cerevisiae. Appl. Environ. Microbiol. 76: 2615-2622.   DOI
16 Kuroda K, Nishitani T, Ueda M. 2012. Specific adsorption of tungstate by cell surface display of the newly designed ModE mutant. Appl. Microbiol. Biotechnol. 96: 153-159.   DOI
17 Kuroda K, Ueda M. 2003 Bioadsorption of cadmium ion by cell surface-engineered yeasts displaying metallothionein and hexa-His. Appl. Microbiol. Biotechnol. 63: 182-186.   DOI
18 Kuroda K, Ueda M. 2006. Effective display of metallothionein tandem repeats on the bioadsorption of cadmium ion. Appl. Microbiol. Biotechnol. 70: 458-463.   DOI
19 Nriagu JO, Pacyna JM 1988. Quantitative assessment of worldwide contamination of air, water and soils by trace metals. Nature 333: 134-139.   DOI
20 Norris PR, Kelly DP. 1977. Accumulation of cadmium and cobalt by Saccharomyces cerevisiae. J. Gen. Microbiol. 99: 317-324.   DOI
21 Parisutham V, Kim TH, Lee SK. 2014. Feasibilities of consolidated bioprocessing microbes: from pretreatment to biofuel production. Bioresour. Technol. 161: 431-440.   DOI
22 Pirzadeh M, Afyuni M, Khoshgoftarmanesh AH. 2012. Status of zinc and cadmium in paddy soils and rice in Isfahan, Fars and Khuzestan Provinces and their effect on food security. JWSS Isfahan Univ. Technol. 16: 81-93.
23 Sanger F, Nicklen S, Coulson AR. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467.   DOI
24 Silver S, Phung LT. 1996. Bacterial heavy metal resistance: new surprises. Annu. Rev. Microbiol. 50: 753-789.   DOI
25 Thirumoorthy N, Manisenthil KT, Sundar AS, Panayappan L, Chatterjee M. 2007. Metallothionein: an overview. World J. Gastroenterol. 13: 993-996.   DOI
26 Tanaka T, Kondo A. 2015 Cell surface engineering of industrial microorganisms for biorefining applications. Biotechnol. Adv. 10: 1016-1025.
27 Tafakori V, Ahmadian G, Amoozegar MA. 2012. Surface display of bacterial metallothioneins and a chitin binding domain on Escherichia coli increase cadmium adsorption and cell immobilization. Appl. Microbiol. Biotechnol. 167: 462-473.
28 Xu W, Huang M, Zhang Y, Yi X, Dong W, Gao X, Jia C. 2011 Novel surface display system for heterogonous proteins on Lactobacillus plantarum. Lett. Appl. Microbiol. 53: 641-648.   DOI
29 Wilde C, Gold ND, Bawa N, Tambor JH, Mougharbel L, Storms R, Martin VJJ. 2012. Expression of a library of fungal β-glucosidases in Saccharomyces cerevisiae for the development of a biomass fermenting strain. Appl. Microbiol. Biotechnol. 95: 647-659.   DOI
30 Wu TJ, Sempos CT, Freudenheim JL, Muti P, Smit E. 2004. Serum iron, copper and zinc concentrations and risk of cancer mortality in US adults. Ann. Epidemiol. 14: 195-201.   DOI
31 Yang T, Zhang XX, Chen ML, Wang JH. 2012 Highly selective preconcentration of ultra-trace cadmium by engineering yeast surface. Analyst 137: 4193-4199.   DOI