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http://dx.doi.org/10.4014/jmb.1509.09067

Extract of Ettlia sp. YC001 Exerts Photoprotective Effects against UVB Irradiation in Normal Human Dermal Fibroblasts  

Lee, Jeong-Ju (Korea Institute for Skin and Clinical Sciences, Konkuk University)
An, Sungkwan (Korea Institute for Skin and Clinical Sciences, Konkuk University)
Kim, Ki Bbeum (Korea Institute for Skin and Clinical Sciences, Konkuk University)
Heo, Jina (Sustainable Bioresource Research Center, KRIBB)
Cho, Dae-Hyun (Sustainable Bioresource Research Center, KRIBB)
Oh, Hee-Mock (Bioenergy and Biochemical Research Center, KRIBB)
Kim, Hee-Sik (Sustainable Bioresource Research Center, KRIBB)
Bae, Seunghee (Korea Institute for Skin and Clinical Sciences, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.4, 2016 , pp. 775-783 More about this Journal
Abstract
The identification of novel reagents that exert a biological ultraviolet (UV)-protective effect in skin cells represents an important strategy for preventing UV-induced skin aging. To this end, we investigated the potential protective effects of Ettlia sp. YC001 extracts against UV-induced cellular damage in normal human dermal fibroblasts (NHDFs). We generated four different extracts from Ettlia sp. YC001, and found that they exhibit low cytotoxicity in NHDFs. The ethyl acetate extract of Ettlia sp. YC001 markedly decreased UVB-induced cytotoxicity. Additionally, the ethyl acetate extract significantly inhibited the production of hydrogen peroxide-induced reactive oxygen species. Moreover, it inhibited UVB-induced thymine dimers, as confirmed by luciferase assay and thymine dimer dot-blot assay. Thus, the study findings suggest Ettlia sp. YC001 extract as a novel photoprotective reagent on UVB-induced cell dysfunctions in NHDFs.
Keywords
Ettlia sp. YC001; dermal fibroblasts; ultraviolet B; photoprotection; photoaging; skin;
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