Browse > Article
http://dx.doi.org/10.4014/jmb.1510.10007

Bacterial Species and Biochemical Characteristic Investigations of Nostoc flagelliforme Concentrates during its Storage  

Yue, Lifang (Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology)
Lv, Hexin (Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology)
Zhen, Jing (Department of Medicinal Chemistry, Ernest Mario School of Pharmacy, Rutgers University)
Jiang, Shengping (Research Center for Modern Analysis Techniques, Tianjin University of Science and Technology)
Jia, Shiru (Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology)
Shen, Shigang (Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology)
Gao, Lu (Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology)
Dai, Yujie (Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.4, 2016 , pp. 648-658 More about this Journal
Abstract
Preservation of fresh algae plays an important role in algae seed subculture and aquaculture. The determination and examination of the changes of cell viability, composition, and bacterial species during storage would help to take suitable preservation methods to prolong the preservation time of fresh algae. Nostoc flagelliforme is a kind of edible cyanobacterium with important herbal and dietary values. This article investigated the changes of bacterial species and biochemical characteristics of fresh N. flagelliforme concentrate during natural storage. It was found that the viability of cells decreased along with the storage time. Fourteen bacteria strains in the algae concentrate were identified by PCR-DGGE and were grouped into four phyla, including Cyanobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Among them, Enterococcus viikkiensis may be a concern in the preservation. Eleven volatile organic compounds were identified from N. flagelliforme cells, in which geosmin could be treated as an indicator of the freshness of N. flagelliforme. The occurrence of indole compound may be an indicator of the degradation of cells.
Keywords
Nostoc flagelliforme; preservation; viability; bacteria; volatile organic compounds;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Andrade MR, Costa JAV. 2008. Outdoor and indoor cultivation of Spirulina platensis in the extreme south of Brazil. Z. Naturforsch. C 63: 85-90.
2 Bondoso J, Balague V, Gasol JM, Lage OM. 2014. Community composition of the Planctomycetes associated with different macroalgae. FEMS Microbiol. Ecol. 88: 445-456.   DOI
3 Casserly C, Erijman L. 2003. Molecular monitoring of microbial diversity in an UASB reactor. Int. Biodeterior. Biodegrad. 52: 7-12.   DOI
4 Chen X, Jia S, Yue S, Wang N, Li C, Wang Y. 2010. Effect of solid bed-materials on vegetative cells of Nostoc flagelliforme. J. Appl. Phycol. 22: 341-347.   DOI
5 Cordero B, Voltolina D. 1997. Viability of mass algal cultures preserved by freezing and freeze-drying. Aquacult. Eng. 16: 205-211.   DOI
6 Day JG, Watanabe MM, Morris GJ, Fleck RA, McLellan MR. 1997. Long-term viability of preserved eukaryotic algae. J. Appl. Phycol. 9: 121-127.   DOI
7 Dias FS, Ramos CL, Schwan RF. 2013. Characterization of spoilage bacteria in pork sausage by PCR-DGGE analysis. Food Sci. Technol. (Campinas) 33: 468-474.   DOI
8 Ding Z, Peng S, Xia W, Zheng H, Chen X, Yin L. 2014. Analysis of five earthy-musty odorants in environmental water by HS-SPME/GC-MS. Int. J. Anal. Chem. 2014: 697260.   DOI
9 Gao K. 1998. Chinese studies on the edible blue-green alga, Nostoc flagelliforme: a review. J. Appl. Phycol. 10: 37-49.   DOI
10 Gao K, Ye C. 2003. Culture of the terrestrial cyanobacterium, Nostoc flagelliforme (Cyanophyceae), under aquatic conditions. J. Phycol. 39: 617-623.   DOI
11 Gram L, Ravn L, Rasch M, Bruhn JB, Christensen AB, Givskov M. 2002. Food spoilage - interactions between food spoilage bacteria. Int. J. Food Microbiol. 78: 79-97.   DOI
12 Grima EM, Pérez JS, Camacho FG, Fernández FA, Alonso DL, Del Castillo CS. 1994. Preservation of the marine microalga, Isochrysis galbana: influence on the fatty acid profile. Aquaculture 123: 377-385.   DOI
13 Harith ZT, Yusoff FM, Shariff M, Ariff AB. 2010. Effect of different separation techniques and storage temperatures on the viability of marine microalgae, Chaetoceros calcitrans, during storage. Biotechnology 9. DOI: 10.3923/biotech.2010.387.391.   DOI
14 Hasegawa M, Nishizawa A, Tsuji K, Kimura S, Harada K-i. 2012. Volatile organic compounds derived from 2-keto-acid decarboxylase in Microcystis aeruginosa. Microbes Environ. 27: 525-528.   DOI
15 Henrikson R. 2010. Spirulina World Food: How This Micro Algae Can Transform Your Health and our Planet. Ronore Enterprises, Incorporated.
16 Hu Z, Zeng X, Wang A, Shi C, Duan D. 2004. An efficient method for DNA isolation from red algae. J. Appl. Phycol. 16: 161-166.   DOI
17 Kampfer P, Schulze R, Jackel U, Malik KA, Amann R, Spring S. 2005. Hydrogenophaga defluvii sp. nov. and Hydrogenophaga atypica sp. nov., isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 55: 341-344.   DOI
18 Kong FZ, Yu RC, Xu ZJ, Zhou MJ. 2012. Application of Excel in calculation of biodiversity indices. Marine Sci. 4: 009.
19 Ma Z, Niu Y, Xie P, Chen J, Tao M, Deng X. 2013. Off-flavor compounds from decaying cyanobacterial blooms of Lake Taihu. J. Environ. Sci. 25: 495-501.   DOI
20 Le Nguyen DD, Ngoc HH, Dijoux D, Loiseau G, Montet D. 2008. Determination of fish origin by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE: an application on Pangasius fish from Viet Nam. Food Control 19: 454-460.   DOI
21 Mishra AK, Shukla E, Singh SS. 2013. Phylogenetic comparison among the heterocystous cyanobacteria based on a polyphasic approach. Protoplasma 250: 77-94.   DOI
22 Moe WM, Stebbing RE, Rao JU, Bowman KS, Nobre MF, da Costa MS, Rainey FA. 2012. Pelosinus defluvii sp. nov., isolated from chlorinated solvent-contaminated groundwater, emended description of the genus Pelosinus and transfer of Sporotalea propionica to Pelosinus propionicus comb. nov. Int. J. Syst. Evol. Microbiol. 62: 1369-1376.   DOI
23 Montaini E, Zittelli GC, Tredici M, Grima EM, Sevilla JF, Pérez JS. 1995. Long-term preservation of Tetraselmis suecica: influence of storage on viability and fatty acid profile. Aquaculture 134: 81-90.   DOI
24 Muyzer G, De Waal EC, Uitterlinden AG. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59: 695-700.
25 Nemec A, Krizova L, Maixnerova M, Reijden TJKVD, Deschaght P, Passet V, et al. 2011. Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with the proposal of Acinetobacter pittii sp. nov. (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU). Res. Microbiol. 162: 393-404.   DOI
26 Schussler W, Nitschke L. 1999. Death of fish due to surface water pollution by liquid manure or untreated wastewater:analytical preservation of evidence by HPLC. Water Res. 33:2884-2887.   DOI
27 Park H. 2006. Long-term preservation of bloom-forming cyanobacteria by cryopreservation. Algae-Inchon 21: 125-132.   DOI
28 Radvanyi D, Gere A, Jokai Z, Fodor P. 2015. Rapid evaluation technique to differentiate mushroom disease-related moulds by detecting microbial volatile organic compounds using HS-SPME-GC-MS. Anal. Bioanal. Chem. 407: 537-545.   DOI
29 Rahkila R, Johansson P, Sade E, Bjorkroth J. 2011. Identification of enterococci from broiler products and a broiler processing plant and description of Enterococcus viikkiensis sp. nov. Appl. Environ. Microbiol. 77: 1196-1203.   DOI
30 Sheffield VC, Cox DR, Lerman LS, Myers RM. 1989. Attachment of a 40-base-pair G+ C-rich sequence (GC-clamp)to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc. Natl. Acad. Sci. USA 86: 232-236.   DOI
31 Song L, Dongi XZ. 2008. Clostridium amylolyticum sp nov., isolated from H(2)-producing UASB granules. Int. J. Syst. Evol. Microbiol. 58: 2132-2135.   DOI
32 Spolaore P, Joannis-Cassan C, Duran E, Isambert A. 2006. Commercial applications of microalgae. J. Biosci. Bioeng. 101:87-96.   DOI
33 Su J, Jia S, Chen X, Yu H. 2008. Morphology, cell growth, and polysaccharide production of Nostoc flagelliforme in liquid suspension culture at different agitation rates. J. Appl. Phycol. 20: 213-217.   DOI
34 Wang J, Zhang J, Ding K, Xin Y, Pang H. 2012. Brevundimonas viscosa sp. nov., isolated from saline soil. Int. J. Syst. Evol. Microbiol. 62: 2475-2479.   DOI
35 Zhao H, Liu J, Dou Q, Su J. 2012. Effects of Nostoc flagelliforme on soil physical-chemical properties and microbial community, pp. 1426-1430.In: Proceedings of the Biomedical Engineering and Biotechnology (iCBEB). 2012 International Conference. 28-30 May 2012.
36 Woo PCY, Teng JLL, Leung KW, Lau SKP, Woo GKS, Wong ACY, et al. 2005. Anaerospora hongkongensis gen. nov sp nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous drug abuser with pseudobacteremia. Microbiol. Immunol. 49: 31-39.   DOI
37 Wu J, Jiang C, Wang BJ, Ma Y, Liu Z, Liu S. 2006. Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1. Appl. Environ. Microbiol. 72: 1759-1765.   DOI
38 Zhang L, Wu C, Ding X, Zheng J, Zhou R. 2014. Characterisation of microbial communities in Chinese liquor fermentation starters Daqu using nested PCR-DGGE. World J. Microbiol. Biotechnol. 30: 3055-3063.   DOI