Browse > Article
http://dx.doi.org/10.4014/jmb.1511.11039

A Leaf-Inhabiting Endophytic Bacterium, Rhodococcus sp. KB6, Enhances Sweet Potato Resistance to Black Rot Disease Caused by Ceratocystis fimbriata  

Hong, Chi Eun (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
Jeong, Haeyoung (Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology)
Jo, Sung Hee (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
Jeong, Jae Cheol (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kwon, Suk Yoon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
An, Donghwan (Department of Agricultural Economics and Rural Development, Research Institute for Agriculture and Life Sciences, Seoul National University)
Park, Jeong Mee (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.3, 2016 , pp. 488-492 More about this Journal
Abstract
Rhodococcus species have become increasingly important owing to their ability to degrade a wide range of toxic chemicals and produce bioactive compounds. Here, we report isolation of the Rhodococcus sp. KB6, which is a new leaf-inhabiting endophytic bacterium that suppresses black rot disease in sweet potato leaves. We determined the 7.0 Mb draft genome sequence of KB6 and have predicted 19 biosynthetic gene clusters for secondary metabolites, including heterobactins, which are a new class of siderophores. Notably, we showed the first internal colonization of host plants with Rhodococcus sp. KB6 and discuss its potential as a biocontrol agent for sustainable agriculture.
Keywords
Bacterial endophyte; biocontrol agent; black rot disease; Rhodococcus sp.; sweet potato;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Bosello M, Zeyadi M, Kraas FI, Linne U, Xie X, Marahiel MA. 2013. Structural characterization of the heterobactin siderophores from Rhodococcus erythropolis PR4 and elucidation of their biosynthetic machinery. J. Nat. Prod. 76: 2282-2290.   DOI
2 Carrano C, Jordan M, Drechsel H, Schmid D, Winkelmann G. 2001. Heterobactins: a new class of siderophores from Rhodococcus erythropolis IGTS8 containing both hydroxamate and catecholate donor groups. Biometals 14: 119-125.   DOI
3 Chiba H, Agematu H, Kaneto R, Terasawa T, Sakai K. 1999. Rhodopeptins (Mer-N1033), novel cyclic tetrapeptides with antifungal activity from Rhodococcus sp. J. Antibiot. 52: 695-699.   DOI
4 Kang SH, Cho H-S, Cheong H, Ryu C-M, Kim JF, Park S-H. 2007. Two bacterial endophytes eliciting both plant growth promotion and plant defense on pepper (Capsicum annuum L.). J. Microbiol. Biotechnol. 17: 96-103.
5 Creason AL, Davis EW, Putnam ML, Vandeputte OM, Chang JH. 2014. Use of whole genome sequences to develop a molecular phylogenetic framework for Rhodococcus fascians and the Rhodococcus genus. Front. Plant Sci. 5: 406.   DOI
6 Engelbrecht CJB, Harrington TC. 2005. Intersterility, morphology and taxonomy of Ceratocystis fimbriata on sweet potato, cacao and sycamore. Mycologia 97: 57-69.   DOI
7 Hong C, Jo S, Moon J, Lee J-S, Kwon S-Y, Park J. 2015. Isolation of novel leaf-inhabiting endophytic bacteria in Arabidopsis thaliana a nd their a ntagonistic effects on phytophathogens. Plant Biotechnol. Rep. 9: 451-458.   DOI
8 Kitagawa W, Ozaki T, Nishioka T, Yasutake Y, Hata M, Nishiyama M, et al. 2013. Cloning and heterologous expression of the aurachin RE biosynthesis gene cluster afford a new cytochrome P450 for quinoline N-hydroxylation. Chembiochem Eur. J. Chem. Biol. 14: 1085-1093.   DOI
9 Muramoto N, Tanaka T, Shimamura T, Mitsukawa N, Hori E, Koda K, et al. 2012. Transgenic sweet potato expressing thionin from barley gives resistance to black rot disease caused by Ceratocystis fimbriata in leaves and storage roots. Plant Cell Rep. 31: 987-997.   DOI
10 Najafipour G, Ebadi N, Ayazpour K. 2014. Phenotypic and genotypic diversity of Rhodococcus fascians, using RAPD-PCR in Fars province. Ind. J. Fund. Appl. Life Sci. 4: 293-302.
11 Park S-Y, Yang S-H, Choi S-K, Kim J-G, Park S-H. 2007. Isolation and characterization of endophytic bacteria from rice root cultivated in Korea. Kor. J. Microbiol. Biotechnol. 35: 1-10.
12 Stes E, Vandeputte OM, Jaziri ME, Holsters M, Vereecke D. 2011. A successful bacterial coup d’État: how Rhodococcus fascians redirects plant development. Ann. Rev. Phytopathol. 49: 69-86.   DOI
13 Rosenblueth M, Martinez-Romero E. 2006. Bacterial endophytes and their interactions with hosts. Mol. Plant Microbe Int. 19: 827-837.   DOI
14 Schneider CA, Rasband WS, Eliceiri KW. 2012. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 9: 671-675.   DOI
15 Seemann T. 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30: 2068-2069.   DOI
16 Von Bargen K, Haas A. 2009. Molecular and infection biology of the horse pathogen Rhodococcus equi. FEMS Microbiol. Rev. 33: 870-891.   DOI
17 Weber T, Blin K, Duddela S, Krug D, Kim HU, Bruccoleri R, et al. 2015. antiSMASH 3.0 — a comprehensive resource for the genome mining of biosynthetic gene clusters. Nucleic Acids Res. 43: W237-W243.   DOI
18 Zerbino DR, Birney E. 2008. Velvet: algorithms for de novoshort read assembly using de Bruijn graphs. Genome Res. 18: 821-829.   DOI