Expression of a Tandemly Arrayed Plectasin Gene from Pseudoplectania nigrella in Pichia pastoris and its Antimicrobial Activity |
Wan, Jin
(Institute of Animal Nutrition, Sichuan Agricultural University)
Li, Yan (Institute of Animal Nutrition, Sichuan Agricultural University) Chen, Daiwen (Institute of Animal Nutrition, Sichuan Agricultural University) Yu, Bing (Institute of Animal Nutrition, Sichuan Agricultural University) Zheng, Ping (Institute of Animal Nutrition, Sichuan Agricultural University) Mao, Xiangbing (Institute of Animal Nutrition, Sichuan Agricultural University) Yu, Jie (Institute of Animal Nutrition, Sichuan Agricultural University) He, Jun (Institute of Animal Nutrition, Sichuan Agricultural University) |
1 | Brinch KS, Sandberg A, Baudoux P, Van Bambeke F, Tulkens PM, Frimodt-Møller N, et al. 2009. Plectasin shows intracellular activity against Staphylococcus aureus in human THP-1 monocytes and in a mouse peritonitis model. Antimicrob. Agents Chemother. 53: 4801-4808. DOI |
2 | Athmaram TN, Saraswat S, Singh AK, Rao MK, Gopalan N, Suryanarayana VVS, Rao PVL. 2012. Influence of copy number on the expression levels of pandemic influenza hemagglutinin recombinant protein in methylotrophic yeast Pichia pastoris. Virus Genes 45: 440-451. DOI |
3 | Barton MD. 2000. Antibiotic use in animal feed and its impact on human health. Nutr. Res. Rev. 13: 279-299. DOI |
4 | Buckholz RG, Gleeson MA. 1991. Yeast systems for the commercial production of heterologous proteins. Nat. Biotechnol. 9: 1067-1072. DOI |
5 | Cereghino JL, Cregg JM. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24: 45-66. DOI |
6 | Dhople V, Krukemeyer A, Ramamoorthy A. 2006. The human beta-defensin-3, an antibacterial peptide with multiple biological functions. BBA Biomembr. 1758: 1499-1512. DOI |
7 | Hancock REW, Patrzykat A. 2002. Clinical development of cationic antimicrobial peptides: from natural to novel antibiotics. Curr. Drug Targets 2: 79-83. DOI |
8 | Hancock REW, Sahl HG. 2006. Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat. Biotechnol. 24: 1551-1557. DOI |
9 | Jing XL, Luo XG, Tian WJ, Lv LH, Jiang Y, Wang N, Zhang TC. 2010. High-level expression of the antimicrobial peptide plectasin in Escherichia coli. Curr. Microbiol. 61: 197-202. DOI |
10 | Hara S, Mukae H, Sakamoto N, Ishimoto H, Amenomori M, Fujita H, et al. 2008. Plectasin has antibacterial activity and no affect on cell viability or IL-8 production. Biochem. Biophys. Res. Commun. 374: 709-713. DOI |
11 | Hunt I. 2005. From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein Expr. Purif. 40: 1-22. DOI |
12 | Iwatani S, Zendo T, Yoneyama F, Nakayama J, Sonomoto K. 2007. Characterization and structure analysis of a novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14. Biosci. Biotechnol. Biochem. 71: 1984-1992. DOI |
13 | Jonasson P, Nygren PÅ, Jörnvall H, Johansson BL, Wahren J, Uhlén M, Ståhl S. 2000. Integrated bioprocess for production of human proinsulin C-peptide via heat release of an intracellular heptameric fusion protein. J. Biotechnol. 76: 215-226. DOI |
14 | Kim H K, Chun DS, Kim J S, Yun CH, Lee JH, Hong SK, Kang DK. 2006. Expression of the cationic antimicrobial peptide lactoferricin fused with the anionic peptide in Escherichia coli. Appl. Microbiol. Biotechnol. 72: 330-338. DOI |
15 | Kim JM, Jang SA, Yu BJ, Sung BH, Cho JH, Kim SC. 2008. High-level expression of an antimicrobial peptide histonin as a natural form by multimerization and furin-mediated cleavage. Appl. Microbiol. Biotechnol. 78: 123-130. DOI |
16 | Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685. DOI |
17 | Liu YF, Luo JC, Xu CY, Ren FC, Peng C, Wu GY, Zhao JD. 2000. Purification, characterization, and molecular cloning of the gene of a seed-specific antimicrobial protein from pokeweed. Plant Physiol. 122: 1015-1024. DOI |
18 | Luo HY, Huang HQ, Yang PL, Wang YR, Yuan TZ, Wu NF, et al. 2007. A novel phytase appA from Citrobacter amalonaticus CGMCC 1696: gene cloning and overexpression in Pichia pastoris. Curr. Microbiol. 55: 185-192. DOI |
19 | Lee SJ, Lee JH, Jin HJ, Lee JH, Ryu HY, Kim Y, et al. 2000. A novel technique for the effective production of short peptide analogs from concatameric short peptide multimers. Mol. Cells 10: 236-240. DOI |
20 | Lewis RV, Hinman M, Kothakota S, Fournier MJ. 1996. Expression and purification of a spider silk protein: a new strategy for producing repetitive proteins. Protein Expr. Purif. 7: 400-406. DOI |
21 | Maemoto A, Qu X, Rosengren KJ, Tanabe H, Henschen-Edman A, Craik DJ, Ouellette AJ. 2004. Functional analysis of the α-defensin disulfide array in mouse cryptdin-4. J. Biol. Chem. 279: 44188-44196. DOI |
22 | Monroe S, Polk R. 2000. Antimicrobial use and bacterial resistance. Curr. Opin. Microbiol. 3: 496-501. DOI |
23 | Murphey ED, Sherwood ER. 2008. Pretreatment with the gram-positive bacterial cell wall molecule peptidoglycan improves bacterial clearance and decreases inflammation and mortality in mice challenged with Pseudomonas aeruginosa. Microbes Infect. 10: 1244-1250. DOI |
24 | Mygind PH, Fischer RL, Schnorr KM, Hansen MT, Sönksen CP, Ludvigsen S, et al. 2005. Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus. Nature 437: 975-980. DOI |
25 | Romanos MA, Scorer CA, Clare JJ. 1992. Foreign gene expression in yeast: a review. Yeast 8: 423-488. DOI |
26 | Schwarz S, Kehrenberg C, Walsh TR. 2001. Use of antimicrobial agents in veterinary medicine and food animal production. Int. J. Antimicrob. Agents 17: 431-437. DOI |
27 | Singh MP, Greenstein M. 2000. A ntibacterial l eads from microbial natural products discovery. Curr. Opin. Drug Discov. Devel. 3: 167-176. |
28 | Schneider T, Kruse T, Wimmer R, Wiedemann I, Sass V, Pag U, et al. 2010. Plectasin, a fungal defensin, targets the bacterial cell wall precursor Lipid II. Science 328: 1168-1172. DOI |
29 | Schnorr KM, Hansen MT, Mygind PH, Segura DR, Hoegenh Kristensen HH, et al. 2011. Antimicrobial polypeptides from Pseudoplectania nigrella. US Patent 7,972,814. |
30 | Smith DL, Harris AD, Johnson JA, Silbergeld EK, Morris JG. 2002. Animal antibiotic use has an early but important impact on the emergence of antibiotic resistance in human commensal bacteria. Proc. Natl. Acad. Sci. USA 99: 6434-6439. DOI |
31 | Tanabe H, Ayabe T, Maemoto A, Ishikawa C, Inaba Y, Sato R, et al. 2007. Denatured human α-defensin attenuates the bactericidal activity and the stability against enzymatic digestion. Biochem. Biophys. Res. Commun. 358: 349-355. DOI |
32 | Tian ZG, Dong TT, Yang YL, Teng D, Wang JH. 2009. Expression of antimicrobial peptide LH multimers in Escherichia coli C43 (DE3). Appl. Microbiol. Biotechnol. 83: 143-149. DOI |
33 | Tian ZG, Teng D, Yang YL, Luo J, Feng XJ, Fan Y, et al. 2007. Multimerization and fusion expression of bovine lactoferricin derivative LfcinB15-W4,10 in Escherichia coli. Appl. Microbiol. Biotechnol. 75: 117-124. DOI |
34 | Vizioli J, Salzet M. 2002. Antimicrobial peptides versus parasitic infections? Trends Parasitol. 18: 475-476. DOI |
35 | Zhang J, Yang YL, Teng D, Tian ZG, Wang SR, Wang JH. 2011. Expression of plectasin in Pichia pastoris and its characterization as a new antimicrobial peptide against Staphyloccocus and Streptococcus. Protein Expr. Purif. 78: 189-196. DOI |
36 | Wang XQ, Yang F, Liu C, Zhou HJ, Wu GY, Qiao SY, et al. 2012. Dietary supplementation with the probiotic Lactobacillus fermentum I5007 and the antibiotic aureomycin differentially affects the small intestinal proteomes of weanling piglets. J. Nutr. 142: 7-13. DOI |
37 | Yamada O, Sakamoto K, Tominaga M, Nakayama T, Koseki T, Fujita A, Akita O. 2005. Cloning and heterologous expression of the antibiotic peptide (ABP) genes from Rhizopus oligosporus NBRC 8631. Biosci. Biotechnol. Biochem. 69: 477-482. DOI |
38 | Zasloff M. 2002. Antimicrobial peptides of multicellular organisms. Nature 415: 389-395. DOI |