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http://dx.doi.org/10.4014/jmb.1507.07086

Regulation of the Lactobacillus Strains on HMGCoA Reductase Gene Transcription in Human HepG2 Cells via Nuclear Factor-κB  

Chen, Kun (College of Pharmaceutical, Liaocheng University)
Li, Shaocong (Department of Gynaecology and Obstetrics, Liaocheng People's Hospital)
Chen, Fang (College of Pharmaceutical, Liaocheng University)
Li, Jun (College of Pharmaceutical, Liaocheng University)
Luo, Xuegang (Key Laboratory of Industrial Microbiology, Ministry of Education, University of Tianjin Science and Technology)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.2, 2016 , pp. 402-407 More about this Journal
Abstract
Lactic acid bacteria have been identified to be effective in reducing cholesterol levels. Most of the mechanistic studies were focused on the bile salt deconjugation ability of bile salt hydrolase in lactic acid bacteria. However, the mechanism by which Lactobacillus decreases cholesterol levels has not been thoroughly studied in intact primate cells. 3-Hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGCR) is the vital enzyme in cholesterol synthesis. To confirm the effect of probiotic Lactobacillus strains on HMGCR level, in the present study, human hepatoma HepG2 cells were treated with Lactobacillus strains, and then the HMGCR level was illustrated by luciferase reporter assay and RT-PCR. The results showed that the level of HMGCR was suppressed after being treated with the live Lactobacillus strains. These works might set a foundation for the following study of the antihyperlipidemic effects of L. acidophilus, and contribute to the development of functional foods or drugs that benefit patients suffering from hyperlipidemia diseases.
Keywords
3-Hydroxy-3-methylglutaryl-coenzyme A reductase; cholesterol; Lactobacillus; nuclear factor κB;
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1 Dambekodi PC, Gilliland SE. 1998. Incorporation of cholesterol into the cellular membrane of Bifidobacterium longum. J. Dairy Sci. 81: 1818-1824.   DOI
2 Boucher P, de Lorgeril M, Salen P, Crozier P, Delaye J, Vallon JJ, et al. 1998. Effect of dietary cholesterol on low density lipoprotein-receptor, 3-hydroxy-3-methylglutaryl-CoA reductase, and low density lipoprotein receptor-related protein mRNA expression in healthy humans. Lipids 33: 1177-1186.   DOI
3 Brown MS, Goldstein JL. 1986. A receptor-mediated pathway for cholesterol homeostasis. Science 232: 34-47.   DOI
4 Chen K, Li J, Luo X, Liao X, Gao J, Hu X, et al. 2012. Induction of nitric oxide synthesis of a Lactobacillus acidophilus strain in human HepG2 cells. Adv. Mater. Res. 365: 194-198.   DOI
5 Kim DW, Cho SB, Yun CH, Jeong HY, Chung WT, Choi CW, et al. 2007. Induction of cytokines and nitric oxide in murine macrophages stimulated with enzymatically digested Lactobacillus strains. J. Microbiol. 45: 373-378.
6 Lin SY, Ayres JW, Winkler W Jr, Sandine WE. 1989. Lactobacillus effects on cholesterol: in vitro and in vivo results. J. Dairy Sci. 72: 2885-2899.   DOI
7 Powell EE, Kroon PA. 1994. Low density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression in human mononuclear leukocytes is regulated coordinately and parallels gene expression in human liver. J. Clin. Invest. 93: 2168-2174.   DOI
8 Liu SF, Ye X, Malik AB. 1999. Inhibition of NF-kappaB activation by pyrrolidine dithiocarbamate prevents in vivo expression of proinflammatory genes. Circulation 100: 1330-1337.   DOI
9 Madrid LV, Wang CY, Guttridge DC, Schottelius AJ, Baldwin AS Jr, Mayo MW. 2000. Akt suppresses apoptosis by stimulating the transactivation potential of the RelA/p65 subunit of NF-κB. Mol. Cell. Biol. 20: 1626-1638.   DOI
10 Pereira DIA, Gibson GR. 2002. Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Appl. Environ. Microbiol. 68: 4689-4693.   DOI
11 Rasic JL, Vujicic IF, Skrinjar M, Vulic M. 1992. Assimilation of cholesterol by some cultures of lactic acid bacteria and bifidobacteria. Biotechnol. Lett. 14: 39-44.   DOI
12 Sambrook J. 2003. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York.
13 Smale ST. 2011. Hierarchies of NF-κB target-gene regulation. Nat. Immunol. 12: 689-694.   DOI
14 Spitzer JA, Zheng M, Kolls JK, Vande Stouwe C, Spitzer JJ. 2002. Ethanol and LPS modulate NF-kappaB activation, inducible NO synthase and COX-2 gene expression in rat liver cells in vivo. Front. Biosci. 7: a99-a108.   DOI
15 Vidon C, Boucher P, Cachefo A, Peroni O, Diraison F, Beylot M. 2001. Effects of isoenergetic high-carbohydrate compared with high-fat diets on human cholesterol synthesis and expression of key regulatory genes of cholesterol metabolism. Am. J. Clin. Nutr. 73: 878-884.   DOI
16 Wan F, Lenardo MJ. 2010. The nuclear signaling of NF-κB: current knowledge, new insights, and future perspectives. Cell Res. 20: 24-33.   DOI