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http://dx.doi.org/10.4014/jmb.1507.07090

Apoptotic Effects of Cordycepin Through the Extrinsic Pathway and p38 MAPK Activation in Human Glioblastoma U87MG Cells

Baik, Ji-Sue (Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University)
Mun, Seo-Won (Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University)
Kim, Kyoung-Sook (Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University)
Park, Shin-Ji (Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University)
Yoon, Hyun-Kyoung (Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University)
Kim, Dong-Hyun (Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University)
Park, Min-Kyu (Department of Clinical Pharmacology & Therapeutics, College of Medicine, Dong-A University)
Kim, Cheorl-Ho (Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University)
Lee, Young-Choon (Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University)

Publication Information

Journal of Microbiology and Biotechnology / v.26, no.2, 2016 , pp. 309-314 More about this Journal

Abstract

We first demonstrated that cordycepin inhibited cell growth and triggered apoptosis in U87MG cells with wild-type p53, but not in T98G cells with mutant-type p53. Western blot data revealed that the levels of procaspase-8, -3, and Bcl-2 were downregulated in cordycepin-treated U87MG cells, whereas the levels of Fas, FasL, Bak, cleaved caspase-3, -8, and cleaved PARP were upregulated, indicating that cordycepin induces apoptosis by activating the death receptor-mediated pathway in U87MG cells. Cordycepin-induced apoptosis could be suppressed by only SB203580, a p38 MAPK-specific inhibitor. These results suggest that cordycepin triggered apoptosis in U87MG cells through p38 MAPK activation and inhibition of the Akt survival pathway.

Keywords

Cordycepin; apoptosis; extrinsic pathway; U87MG cell; p38 MAPK;

Citations & Related Records

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