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http://dx.doi.org/10.4014/jmb.1505.05028

Immunization of Mice with Recombinant Brucella abortus Organic Hydroperoxide Resistance (Ohr) Protein Protects Against a Virulent Brucella abortus 544 Infection  

Hop, Huynh Tan (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Reyes, Alisha Wehdnesday Bernardo (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Simborio, Hannah Leah Tadeja (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Arayan, Lauren Togonon (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Min, Won Gi (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Lee, Hu Jang (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Lee, Jin Ju (Animal and Plant Quarantine Agency)
Chang, Hong Hee (Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Suk (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.1, 2016 , pp. 190-196 More about this Journal
Abstract
In this study, the Brucella abortus ohr gene coding for an organic hydroperoxide resistance protein (Ohr) was cloned into a maltose fusion protein expression system (pMAL), inserted into Escherichia coli, and purified, and its immunogenicity was evaluated by western blot analysis using Brucella-positive mouse sera. The purified recombinant Ohr (rOhr) was treated with adjuvant and injected intraperitoneally into BALB/c mice. A protective immune response analysis revealed that rOhr induced a significant increase in both the IgG1 and IgG2a titers, and IgG2a reached a higher level than IgG1 after the second and third immunizations. Additionally, immunization with rOhr induced high production of IFN-γ as well as proinflammatory cytokines such as TNF, MCP-1, IL-12p70, and IL-6, but a lesser amount of IL-10, suggesting that rOhr predominantly elicited a cell-mediated immune response. In addition, immunization with rOhr caused a significantly higher degree of protection against a virulent B. abortus infection compared with a positive control group consisting of mice immunized with maltose-binding protein. These findings showed that B. abortus rOhr was able to induce both humoral and cell-mediated immunity in mice, which suggested that this recombinant protein could be a potential vaccine candidate for animal brucellosis.
Keywords
Brucella abortus; organic hydroperoxide resistance protein (Ohr); immunization; vaccine;
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